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Lyon J.P.,Federal University of Sao Joao del Rei | de Pedroso e Silva Azevedo C.M.,Federal University of Maranhao | Moreira L.M.,Federal University of Sao Joao del Rei | de Lima C.J.,Parque Tecnologico Sao Jose dos Campos | de Resende M.A.,Federal University of Minas Gerais
Mycopathologia | Year: 2011

Photodynamic therapy (PDT) is a minimally invasive approach, in which a photosensitizer compound is activated by exposure to light. The activation of the sensitizer drug results in several chemical reactions, such as the production of reactive oxygen species and other reactive molecules, which presence in the biological site leads to the damage of target cells. Although PDT has been primarily developed to combat cancerous lesions, this therapy can be employed for the treatment of several conditions, including infectious diseases. A wide range of microorganisms, including Gram-positive and Gram-negative bacteria, viruses, protozoa, and fungi, have demonstrated susceptibility to antimicrobial PDT. This treatment might consist in an alternative for the management of fungal infections. Antifungal photodynamic therapy has been successfully employed against Candida species, dermatophytes, and Aspergillus niger. Chromoblastomycosis is an infection that involves skin and subcutaneous tissues caused by the traumatic inoculation of dematiaceous fungi species, being that the most prevalent are Fonsecaea pedrosoi and Claphialophora carrionii. In the present work, the clinical applications of PDT for the treatment of chromoblastomycosis are evaluated. We have employed methylene blue as photosensitizer and a LED (Light Emitting Diode) device as light source. The results of this treatment are positive, denoting the efficacy of PDT against chromoblastomycosis. Considering that great part of the published works are focused on in vitro trials, these clinical tests can be considered a relevant source of information about antifungal PDT, since its results have demonstrated to be promising. The perspectives of this kind of treatment are analyzed in agreement with the recent literature involving antifungal PDT. © 2011 Springer Science+Business Media B.V.


De Siqueira E Oliveira F.S.,Paulista University | Giana H.E.,Laboratorio Oswaldo Cruz | Silveira Jr. L.,Parque Tecnologico Sao Jose dos Campos
Journal of Biomedical Optics | Year: 2012

A method, based on Raman spectroscopy, for identification of different microorganisms involved in bacterial urinary tract infections has been proposed. Spectra were collected from different bacterial colonies (Gramnegative: Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Enterobacter cloacae, and Gram-positive: Staphylococcus aureus and Enterococcus spp.), grown on culture medium (agar), using a Raman spectrometer with a fiber Raman probe (830 nm). Colonies were scraped from the agar surface and placed on an aluminum foil for Raman measurements. After preprocessing, spectra were submitted to a principal component analysis and Mahalanobis distance (PCA/MD) discrimination algorithm. We found that the mean Raman spectra of different bacterial species show similar bands, and S. aureus was well characterized by strong bands related to carotenoids. PCA/MD could discriminate Gram-positive bacteria with sensitivity and specificity of 100% and Gram-negative bacteria with sensitivity ranging from 58 to 88% and specificity ranging from 87% to 99%. © 2012 Society of Photo-Optical Instrumentation Engineers (SPIE).


Conrado L.A.L.,Parque Tecnologico Sao Jose dos Campos | Munin E.,Parque Tecnologico Sao Jose dos Campos
Journal of Cosmetic Dermatology | Year: 2013

Background: Published literature reports significant improvements in pathological conditions, such as pain, blood dyscrasias, and cellulite, after using topical occlusive accessories containing particulate ceramic materials. Recent work reported a reduction in body measurements after 30 days of daily use of high-waist undershorts made of synthetic fabrics with embedded ceramic particles. Objectives: In this study, we investigated whether the use of a garment made with synthetic fibers embedded with powdered ceramic could lead to reductions in body measurements at a longer time span (120 day). Methods: Thirty-two female volunteers wore clothing containing ceramic powder for at least 8 h a day for 120 days. The efficacy of the treatment was evaluated through anthropometric measurements taken before treatment and after 30, 60, and 120 days of treatment. Results: The experimental data showed a more pronounced reduction in body measurements at the beginning of treatment with a trend of stabilization in measured body circumferences during the final 60 days of the experimental period. Conclusions: The experimental data showed that the occlusive treatment with a garment composed of ceramic particles promoted reductions in body measurements during a 120-day experimental period. © 2013 Wiley Periodicals, Inc.


Campos L.A.,Parque Tecnologico Sao Jose dos Campos | Campos L.A.,Victor Babes University of Medicine and Pharmacy Timisoara | Bader M.,Max Delbruck Center for Molecular Medicine | Baltatu O.C.,Parque Tecnologico Sao Jose dos Campos | Baltatu O.C.,Victor Babes University of Medicine and Pharmacy Timisoara
Frontiers in Physiology | Year: 2012

Brain renin-angiotensin system (RAS) is significantly involved in the roles of the endocrine RAS in cardiovascular regulation. Our studies indicate that the brain RAS participates in the development of cardiac hypertrophy and fibrosis through sympathetic activation. Inhibition of sympathetic hyperactivity after myocardial infarction through suppression of the brain RAS appears beneficial. Furthermore, the brain RAS modulates the cardiovascular and fluid-electrolyte homeostasis not only by interacting with the autonomic nervous system but also by modulating hypothalamic-pituitary axis and vasopressin release. The brain RAS is also involved in the modulation of circadian rhythms of arterial pressure, contributing to non-dipping hypertension. We conclude that the brain RAS in pathophysiological states interacts synergistically with the chronically overactive RAS through a positive biofeedback in order to maintain a state of alert in diseased conditions, such as cardiac hypertrophy and failure. Therefore, targeting brain RAS with drugs such as renin or angiotensin converting enzyme inhibitors or receptor blockers having increased brain penetrability could be of advantage. © 2012 Campos, Bader and Baltatu.


Conrado L.A.L.,Parque Tecnologico Sao Jose dos Campos | Munin E.,Parque Tecnologico Sao Jose dos Campos
Journal of Cosmetic Dermatology | Year: 2011

Background: Therapeutic effects have been attributed to far-infrared (FIR) radiation emitted from different types of sources. One class of such infrared emitters consists of ceramics in a powdered form, which allows for its incorporation into creams and fabrics. Such ceramic materials emit in the FIR when subjected to body temperature. Published literature reports significant improvement in both pathological conditions, such as pain and blood dyscrasias, and cellulite upon use of accessories containing ceramic emitters. Objectives: In this study, we investigated whether the use of a garment made with synthetic fibers embedded with powdered ceramic led to a reduction in body measurements. Methods: The study population comprised 42 women divided into two groups: active and placebo. The volunteers used clothing either impregnated or not impregnated with ceramic powder for at least 8h/day for 30days. Results: The experimental data showed a reduction in body measurements, which may be a consequence of an increment in microcirculation and peripheral blood flow, and these changes might promote improved general health. Conclusions: Objective indicators were identified which showed that the reported ceramic accessories actually were capable of biological modulation. © 2011 Wiley Periodicals, Inc.

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