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Rawat J.M.,Forest Research Institute FRI | Rawat J.M.,Parnkuti Anantpur University Road | Rawat J.M.,Central Institute of Medicinal and Aromatic Plants | Rawat B.,Gb Pant Institute Of Himalayan Environment And Development | And 2 more authors.
Biotechnology Letters | Year: 2013

Among five hairy root lines of Picrorhiza kurrooa that were established through Agrobacterium rhizogenes, one (H7) was selected for encapsulation due to high accumulation of picrotin and picrotoxinin (8.3 and 47.6 μg/g DW, respectively). Re-grown encapsulated roots induced adventitious shoots with 73 % frequency on MS medium supplemented with 0.1 μM 6-benzylaminopurine, following 6 months of storage at 25 °C. Regenerated plantlets had 85 % survival after 2 months. Regenerants were of similar morphotype having increased leaf number and branched root system as compared to non-transformed plants. The transformed nature of the plants was confirmed through PCR and Southern blot analysis. Genetic fidelity analysis of transformed plants using RAPD and ISSR showed 5.2 and 3.6 % polymorphism, respectively. Phytochemical analysis also showed that picrotin and picrotoxinin content were similar in hairy root line and its regenerants. © 2013 Springer Science+Business Media Dordrecht.

Rawat B.,Gb Pant Institute Of Himalayan Environment And Development | Rawat B.,Parnkuti Anantpur University Road | Gairola S.,University of Sharjah | Rawal R.S.,Gb Pant Institute Of Himalayan Environment And Development
Journal of Mountain Science | Year: 2015

Present study has been conducted in a biodiversity rich Nanda Devi Biosphere Reserve between 2000-3800 m in two different forest physiognomy holding sites to analyze the structure and composition of the forest communities including richness of native, non native and endemic species, and suggest conservation values at community and reserve level. A total of 60 sites were sampled and grouped in 11 and 8 communities for two representative sites Pindari-Sunderdhunga-Kafni (PSK) and Lata-Tolma-Phagti (LTP) respectively. From the sampled sites, 451 species (11.8%, 53 trees; 17.71%, 80 shrubs; and 70.51%, 318 herbs) have been recorded. In general, PSK site represented 73.6% and LTP site represented only 54.9% of the total plant species recorded in the study area. Out of total species in PSK site, native species contributed 59.6% (198 spp.) and 24.7% species were endemic and near endemic. In LTP site, 66.5% species were Himalayan natives and 33.5% were endemic and near endemic. The species richness ranged from 4–23 ind ha−1 (tree), 3–18 ind ha−1 (sapling), 5–19 ind ha−1 (seedling) in PSK site and 4–18 ind ha−1 (tree), 4–11 ind ha−1 (sapling), 4–16 ind ha−1 (seedling) in LTP site. The density ranged from 260–535 ind ha−1 (tree), 145–633 ind ha−1 (sapling), 1450–8170 ind ha−1 (seedling) in PSK site and 599–1211 ind ha−1 (tree), 70–951 ind ha−1 (sapling), 470–1665 ind ha−1 (seedling) in LTP site. Species diversity for trees ranged from 0.73–3.37, saplings, 0.64–2.67, seedlings, 0.70–2.51, shrubs, 1–2.34 and herbs, 2.02–3.21 in PSK site and 0.63–1.61, saplings, 0.76–1.36, seedlings, 0.35–1.79, shrubs, 0.98–2.73 and herbs, 2.48–3.61 in LTP site. These recorded values were almost comparable with the studies conducted in sub-tropical, temperate and sub-alpine regions of the west Himalaya. In some cases the values were slightly higher than the reported values. The important communities with high conservation values have been identified based on different ecological parameters and species distribution. Among all the prioritized communities, Mixed Silver fir-Rhododendron-Maple community in PSK site and Taxus wallichana- A. pindrow mixed community in LTP site supports maximum richness and density of native and endemic species. This study calls for development of adequate strategy and action plan for the conservation and management of habitats, species, and communities under changing climate and socioeconomic scenarios, so that sustainable utilization of the species could be ensured. © 2015, Science Press, Institute of Mountain Hazards and Environment, CAS and Springer-Verlag Berlin Heidelberg.

Rawat B.,Gb Pant Institute Of Himalayan Environment And Development | Rawat B.,Forest Research Institute | Rawat B.,Parnkuti Anantpur University Road | Gairola S.,Gb Pant Institute Of Himalayan Environment And Development | And 2 more authors.
Journal of Forestry Research | Year: 2015

We studied forest vegetation at Lata–Tolma–Phagti, a protected area and part of Nanda Devi Biosphere Reserve in the western Himalaya. We analyzed community composition, population structure, regeneration patterns, and projected development of future compositional patterns. We sampled ten 10 × 10 m quadrat for tree species in each of 30 forest stands. We sampled shrubs in ten 5 × 5 m quadrat, and herbs in twenty 1 × 1 m quadrat within each forest stand. We recorded 248 plant species from 8 forest communities. Broadly the demographic profiles at study sites exhibited progressive structures suggesting long term persistence of the communities/species. Density–diameter distribution revealed greater proportions of seedlings and a significant decline (P < 0.05) in the proportion of trees in older age/size classes. Of the 23 recorded tree species, 13.0 % showed good, 52.2 % fair, 26.1 % poor and 8.7 % no regeneration. Differences in regeneration by species are indicative of future forest structure and dynamics. Assessment of changes in structure of forest types provides baseline data for development of priorities for conservation of other representative landscapes in the reserve as well as in the Himalaya. © 2015 Northeast Forestry University and Springer-Verlag Berlin Heidelberg

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