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Deaguero A.L.,Parker H Petit Institute Of Bioengineering And Biosciences | Bommarius A.S.,Parker H Petit Institute Of Bioengineering And Biosciences
Biotechnology and Bioengineering

Ethylene glycol has the ability to act as a reactant when employed as a co-solvent for the enzymatic synthesis of ampicillin from (R)-phenylglycine methyl ester and 6-aminopenicillanic acid. The side reaction positively affects yield because its product, (R)-phenylglycine hydroxyethyl ester, is an intermediate for ampicillin synthesis. Biotechnol. Biotechnol. Bioeng. 2014;111: 1054-1058. © 2013 Wiley Periodicals, Inc. Source

Jabbari N.,Parker H Petit Institute Of Bioengineering And Biosciences | Reavis A.N.,Parker H Petit Institute Of Bioengineering And Biosciences | McDonald J.F.,Parker H Petit Institute Of Bioengineering And Biosciences | McDonald J.F.,Georgia Institute of Technology
Journal of Ovarian Research

Background: Epithelial-Mesenchymal Transition (EMT) is a transient and reversible (Mesenchymal-Epithelial Transition or MET) process by which epithelial cells acquire mesenchymal cell characteristics including reduced intercellular adhesion and increased cell motility. While EMT/MET has long been recognized as an essential component of early embryonic development, there is a growing body of evidence indicating that EMT/MET is also a key component of ovarian cancer (OC) metastasis. Recent findings have implicated members of the miR-200 family of microRNAs (miRNAs) in this process. Methods. Individual members of the miR-200 family of miRNAs were transiently over expressed in metastatic (mesenchymal-like) OC cell lines. Changes in morphology, molecular profiles and drug sensitivity were monitored relative to cells transfected with a negative control. Results: Morphological hallmarks of MET were detected in cells transfected with all miR-200 family members. Gene expression profiling demonstrated up regulation of epithelial biomarkers and down regulation of mesenchymal biomarkers in transfected cells although significant variation in molecular response and drug sensitivity was associated with different members of the miR-200 family. Conclusions: Our results indicate that although ectopic overexpression of all members of the miR-200 family in mesenchymal-like OC cells results in morphological changes characteristic of MET, the underlying molecular changes and induced drug sensitivities are highly variable and correlated with sequence variation within the seed and non-seed regions of individual family members. © 2014 Jabbari et al.; licensee BioMed Central Ltd. Source

Lili L.N.,Parker H Petit Institute Of Bioengineering And Biosciences | Matyunina L.V.,Parker H Petit Institute Of Bioengineering And Biosciences | Walker L.D.,Parker H Petit Institute Of Bioengineering And Biosciences | Daneker G.W.,Cancer Treatment Centers of America Regional Facility | McDonald J.F.,Parker H Petit Institute Of Bioengineering And Biosciences

OBJECTIVES: There is a growing body of evidence that targeted gene therapy holds great promise for the future treatment of cancer. A crucial step in this therapy is the accurate identification of appropriate candidate genes/pathways for targeted treatment. One approach is to identify variant genes/pathways that are significantly enriched in groups of afflicted individuals relative to control subjects. However, if there are multiple molecular pathways to the same cancer, the molecular determinants of the disease may be heterogeneous among individuals and possibly go undetected by group analyses. METHODS: In an effort to explore this question in pancreatic cancer, we compared the most significantly differentially expressed genes/pathways between cancer and control patient samples as determined by group versus personalized analyses. RESULTS: We found little to no overlap between genes/pathways identified by gene expression profiling using group analyses relative to those identified by personalized analyses. CONCLUSIONS: Our results indicate that personalized and not group molecular profiling is the most appropriate approach for the identification of putative candidates for targeted gene therapy of pancreatic and perhaps other cancers with heterogeneous molecular etiology. © 2014 by Lippincott Williams and Wilkins. Source

Kundu K.,Parker H Petit Institute Of Bioengineering And Biosciences | Knight S.F.,Emory University | Lee S.,Parker H Petit Institute Of Bioengineering And Biosciences | Taylor W.R.,Parker H Petit Institute Of Bioengineering And Biosciences | And 3 more authors.
Angewandte Chemie - International Edition

A breath of fresh air: The rate of aerial oxidation of dihydroethidium and hydrocyanine radical oxidant probes can be selectively reduced by deuteration (see picture). The reaction rate between the deuterated compounds and the superoxide radical was reduced by a much smaller factor because of mechanistic differences between the two reactions. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim. Source

Zang X.,Integrated Cancer Research Center | Jones C.M.,Integrated Cancer Research Center | Long T.Q.,Integrated Cancer Research Center | Monge M.E.,Integrated Cancer Research Center | And 9 more authors.
Journal of Proteome Research

Prostate cancer (PCa) is the second leading cause of cancer-related mortality in men. The prevalent diagnosis method is based on the serum prostate-specific antigen (PSA) screening test, which suffers from low specificity, overdiagnosis, and overtreatment. In this work, untargeted metabolomic profiling of age-matched serum samples from prostate cancer patients and healthy individuals was performed using ultraperformance liquid chromatography coupled to high-resolution tandem mass spectrometry (UPLC-MS/MS) and machine learning methods. A metabolite-based in vitro diagnostic multivariate index assay (IVDMIA) was developed to predict the presence of PCa in serum samples with high classification sensitivity, specificity, and accuracy. A panel of 40 metabolic spectral features was found to be differential with 92.1% sensitivity, 94.3% specificity, and 93.0% accuracy. The performance of the IVDMIA was higher than the prevalent PSA test. Within the discriminant panel, 31 metabolites were identified by MS and MS/MS, with 10 further confirmed chromatographically by standards. Numerous discriminant metabolites were mapped in the steroid hormone biosynthesis pathway. The identification of fatty acids, amino acids, lysophospholipids, and bile acids provided further insights into the metabolic alterations associated with the disease. With additional work, the results presented here show great potential toward implementation in clinical settings. © 2014 American Chemical Society. Source

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