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Jan S.A.,PARC Institute of Advanced Studies in Agriculture PIASA | Shah S.H.,Allama Iqbal Open University | Ali S.,Pakistan National Agricultural Research Center | Ali G.M.,Pakistan National Agricultural Research Center
Pakistan Journal of Botany | Year: 2015

Efficient tissue culture system is important for transformation of important genes in hybrid tomato cultivars. The present study was undertaken to develop an efficient tissue culture system for hybrid tomato cultivar Peto-86. The young primary leaves and stems were inoculated into five different MS media having different concentrations of plant growth regulators in different combinations for callus induction, somatic embryogenesis and for both direct and indirect regeneration. Maximum callus induction frequency 90% was achieved with MS media containing 2,4-D 4 mg L-1 and BAP 0.5 mg L-1. The direct somatic embryogenesis was found highest on MS media supplemented with 2,4-D 4 mg L-1 and BAP 0.5 mg L-1. Maximum indirect regeneration frequency 87% was achieved from primary leaves explants with MS media containing IAA 0.5 mg L-1 and BAP 3 mg L-1 and highest direct regeneration frequency 77% was obtained from primary leaves explants with MS media containing NAA 1 mg L-1 and BAP 3 mg L-1. The high concentration of 2,4-D increased callus induction and somatic embryogenesis frequencies while the high concentration of BAP increased regeneration frequency. An improved tissue culture system of hybrid tomato cultivar Peto-86 was established and it may be recommended for further transformation experiments. © 2015, Pakistan Botanical Society. All rights reserved. Source


Shah S.H.,Allama Iqbal Open University | Shah S.H.,Gomal University | Khan E.A.,Gomal University | Shah H.,Crop Diseases Research Institute CDRI | And 3 more authors.
Pakistan Journal of Botany | Year: 2016

Allelopathy provides eco-friendly environment in managing weeds by reducing the use of synthetic herbicides that cause environmental pollution and herbicide resistance problems. Therefore, weeds have been controlling by plant derived organic compounds as an alternative of inorganic herbicides since the last two decades. In this study, sorghum aqueous extracts were applied individually as well as accumulatively with reduced levels of Dual Gold® (S-Metolachlor) as foliar sprays in sunflower at 50, 70 and 90 DAS. For comparison, standard level of S-Metolachlor was also applied as foliar sprays along with weedy check. The highest reduction of total weed density (93.7%) was recorded by three sprays of sorghum aqueous extracts at rate of 15 L/ha mixed with 1/3rd S-Metolachlor at 1.6 L/ha as foliar applications. This reduction rate was statistically similar to one that was obtained by standard level of S-Metolachlor (1.6 L/ha). The highest achene yield was achieved by applying three foliar sprays of aqueous sorghum extracts along with reduced doses of S-Metolachlor, which was almost similar to full recommended dose of S-Metolachlor. These findings demonstrate that allelopathy offers environment friendly and economical opportunity for weed control in sunflower reducing the dependence and cost of herbicides. © 2016, Pakistan Botanical Society. All Rights reserved. Source


Bahadur A.,Hazara University | Mushtaq T.,Hazara University | Danish M.,Hazara University | Ullah I.,Hazara University | And 5 more authors.
International Journal of Phytomedicine | Year: 2013

The traditional utilization of medicinal plants in healthcare performs and providing indication to new areas of research and hence its importance is now well recognized. However, information on the uses of indigenous plants for medicine is not well documented from many rural areas of Pakistan including district Mardan. The present studies were aimed to explore the ethnomedicinal profile and conservation status of threatened flora of Jahangir abad. The study area is located in the district Mardan, Khyber Pakhtunkhwa, Pakistan, contains more than 90 species directly used by local communities. Of these plants, 51 are wild plants, 23 cultivated vegetable crops and 16 are important medicinal and economically important trees. Menta longifolia, Calotropis procera, Solanum surretense, Allium sativum and Melia azedarach had multipurpose medicinal uses. Different pathological effect and pitiless collection of medicinal plants in the area has threatened the survival of some essential and valuable medicinal plants like Daibergia sissooand Tamarixindica in the area of Jahangir abad. Source


Muhammad I.,PARC Institute of Advanced Studies in Agriculture PIASA | Zia M.A.,PARC Institute of Advanced Studies in Agriculture PIASA | Zia M.A.,Pakistan National Agricultural Research Center | Ali S.,PARC Institute of Advanced Studies in Agriculture PIASA | And 7 more authors.
International Journal of Agriculture and Biology | Year: 2014

Establishment of an efficient and reproducible regeneration system in three different rice cultivars, IRRI-6, IRRI-9 and KSK-282 remained obscured. In the present study regeneration system was developed for all three recalcitrant cultivars and resultant putative transgenic plants were analyzed for expression by reverse transcriptase polymerase chain reaction (RT-PCR). Cultivars responded to a particular concentration of 2, 4-D ranging from 2 to 4mg L-1 for calli initiation and induction. Two types of calli with different frequencies were observed on both N6 and N6 supplemented with proline, glycine and MES. N6 media produced more number of type II calli while N6 supplemented favored type I calli in all three cultivars. Type I calli were found as yellowish, smaller in size and globular shape and better explants for transformation. GUS histochemical assay showed that 11, 13 and 14 days older type I calli of KSK-282, IRRI-6 and IRRI-9were suitable for transformation respectively. Fifteen minutes infection time and 2 days co-cultivation period showed the highest GUS positive expression in calli of all three cultivars. KSK-282 and IRRI-9 showed best regeneration response on 0.5 mg L-1 kinetin, 1 mg L-1 NAA and 3 mg L-1 BAP, while the IRRI-6 showed the highest regeneration at 0.5 mg L-1 kinetin, 1mg L-1 NAA and 2 mg L-1 BAP. Using optimized protocol for each cultivar GUS reporter gene has been successfully incorporated in the genome of all three cultivars. Transgene expression in resultant lines was confirmed at transcriptional level through reverse transcriptase polymerase chain reaction (RT-PCR). © 2014 Friends Science Publishers. Source

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