Gimenez G.,Parasitologia e Inmunologia |
Magalhaes K.G.,Laboratorio Of Imunofarmacologia |
Belaunzaran M.L.,Parasitologia e Inmunologia |
Poncini C.V.,Parasitologia e Inmunologia |
And 4 more authors.
Molecular Immunology | Year: 2010
Babesia bovis is an intraerythrocytic apicomplexan protozoa of cattle that causes an acute infection with parasite persistence. Babesiosis limitation depends on macrophages, essential effector cells of the host innate defense, which generate inflammatory cytokines and nitric oxide. Herein, we report quantitative differences in the lipid composition of merozoites from two B. bovis strains with polar behaviour: attenuated R1A and virulent S2P. Accordingly, we observed a distinct inflammatory response induced by the total lipids of R1A (LA) and S2P (LV) in murine peritoneal macrophages. LA and particularly its fractions phosphatidic acid and phosphatidylserine + phosphatidylinositol (PS + PI), produced a strong activation of these cells with lipid body formation, cyclooxygenase-2 expression and pro-inflammatory TNFα, IL-6 and KC secretion. Although LV did not activate these cells, the corresponding PS + PI fraction induced TNFα, IL-6 and KC release. Therefore, these facts might be suggesting the presence of an inhibitor in LV. Furthermore, the employment of wild type and toll like receptor 2 knockout (TLR2KO) mice allowed us to demonstrate that macrophage activation by the stimulating lipid fractions was mediated through TLR2. Interestingly, only LA activated the extracellular signal-regulated kinases 1 and 2 (ERK1/2). Inhibitory studies employing UO126, indicated that the ERK pathway was required for TNFα, IL-6 and KC release. In conclusion, the absence of inflammatory response observed with the lipids of S2P virulent strain could constitute an evasion mechanism of the innate immune response enabling parasite establishment in the host. © 2010 Elsevier Ltd. All rights reserved.