Parasite Technologies A S

Hørsholm, Denmark

Parasite Technologies A S

Hørsholm, Denmark
SEARCH FILTERS
Time filter
Source Type

Vejzagic N.,Copenhagen University | Kringel H.,Parasite Technologies A S | Bruun J.M.,Parasite Technologies A S | Roepstorff A.,Parasite Technologies A S | And 4 more authors.
Veterinary Parasitology | Year: 2016

The therapeutic potential of infective pig whipworm eggs, Trichuris suis ova (TSO), is currently tested in several clinical trials on immune-mediated diseases. This paper studied the embryonic development of TSO in a medicinal raw product, where the parasite eggs were suspended in sulphuric acid (pH1). Unembryonated T. suis egg batches were stored at 5, 10, 15, 20, 25, 30, and 40 °C (±1 °C) and examined at 2, 4, 8, and 14 weeks. Subsequently, sub-batches from each temperature were allowed to embryonate for additional 14 weeks at 25 °C, and selected samples were tested for infectivity in Göttingen minipigs.Both male and female pigs were used to evaluate eventual gender specific infectivity. Storage at 30 °C up to 14 weeks and subsequent embryonation for 14 weeks at 25 °C did not significantly reduce the overall larval establishment in minipigs, as compared to storage at 5 °C and subsequent embryonation at 25 °C. As marked impairment of egg development was observed during storage at 40 °C, a second set of unembryonated egg batches were incubated at 30, 32, 34, 36, 38, and 40 °C (±1 °C) for 1-8 weeks. The development of the eggs was repeatedly examined by manual light microscopy, multispectral analysis (OvaSpec), and an egg hatching assay prior to the final testing in minipigs (Trial 1). These methods showed that the development started earlier at higher temperatures, but the long-term storage at higher temperature affected the egg development. The present study further documents tolerance of the TSO to storage at temperature 5-15 °C, at which temperature development of larvae is not initiated. © 2015 Elsevier B.V.


PubMed | Parasite Technologies A S, Ellegaard Gottingen Minipigs and Copenhagen University
Type: | Journal: Veterinary parasitology | Year: 2016

The therapeutic potential of infective pig whipworm eggs, Trichuris suis ova (TSO), is currently tested in several clinical trials on immune-mediated diseases. This paper studied the embryonic development of TSO in a medicinal raw product, where the parasite eggs were suspended in sulphuric acid (pH1). Unembryonated T. suis egg batches were stored at 5, 10, 15, 20, 25, 30, and 40C (1C) and examined at 2, 4, 8, and 14 weeks. Subsequently, sub-batches from each temperature were allowed to embryonate for additional 14 weeks at 25C, and selected samples were tested for infectivity in Gttingen minipigs. Both male and female pigs were used to evaluate eventual gender specific infectivity. Storage at 30C up to 14 weeks and subsequent embryonation for 14 weeks at 25C did not significantly reduce the overall larval establishment in minipigs, as compared to storage at 5C and subsequent embryonation at 25C. As marked impairment of egg development was observed during storage at 40C, a second set of unembryonated egg batches were incubated at 30, 32, 34, 36, 38, and 40C (1C) for 1-8 weeks. The development of the eggs was repeatedly examined by manual light microscopy, multispectral analysis (OvaSpec), and an egg hatching assay prior to the final testing in minipigs (Trial 1). These methods showed that the development started earlier at higher temperatures, but the long-term storage at higher temperature affected the egg development. The present study further documents tolerance of the TSO to storage at temperature 5-15C, at which temperature development of larvae is not initiated.


Vejzagic N.,Copenhagen University | Adelfio R.,Swiss Tropical and Public Health Institute | Adelfio R.,University of Basel | Keiser J.,Swiss Tropical and Public Health Institute | And 5 more authors.
Parasites and Vectors | Year: 2015

Background: Eggs of the porcine whipworm Trichuris suis are currently explored in human clinical trials as a treatment of immune-mediated diseases. In this context, only the infective, embryonated eggs, constitute the Active Pharmaceutical Ingredient (API). The rodent whipworm, Trichuris muris is commonly used as a laboratory model to study Trichuris biology. The embryonated eggs (containing a fully developed larva) are biologically active and will invade the large intestinal mucosa of the host. This study aims to assess the in vitro hatching of T. muris and T. suis eggs in various bacterial cultures as a measure for their biological activity. Methods: Eggs of T. muris and T. suis were incubated with Escherichia coli strain (BL-21) at three concentrations in a slightly modified in vitro egg hatching assay previously developed for T. muris. Additionally, E. coli strains (M15, SG13009, PMC103, JM109, TUNER, DH5alpha, TOP10) and five Gram-positive bacteria (Enterococcus caccae, Streptococcus hyointestinalis, Lactobacillus amylovorus, L. murinus, and L. reuteri) were tested as a hatching stimulus for T. muris and T. suis eggs. Results: Whereas T. muris eggs hatched, T. suis did not, even when exposed to different concentrations and strains of E. coli after 4 and 24-hour incubation. When incubated with Gram-positive bacteria, only T. muris eggs showed noticeable hatching after 20 h, although with high variability. Conclusions: The observed difference in hatching of T. muris and T. suis eggs incubated with selected bacteria, indicate significant biological differences which may reflect specific adaptation to different host-specific gut microbiota. © 2015 Vejzagić et al.


Bruun J.M.,Copenhagen University | Carstensen J.M.,Copenhagen University | Vejzagic N.,Copenhagen University | Christensen S.,Copenhagen University | And 2 more authors.
Computers in Biology and Medicine | Year: 2014

Background: OvaSpec is a new, fully automated, vision-based instrument for assessing the quantity (concentration) and quality (embryonation percentage) of Trichuris suis parasite eggs in liquid suspension. The eggs constitute the active pharmaceutical ingredient in a medicinal drug for the treatment of immune-mediated diseases such as Crohn[U+05F3]s disease, ulcerative colitis, and multiple sclerosis. Methods: This paper describes the development of an automated microscopy technology, including methodological challenges and design decisions of relevance for the future development of comparable vision-based instruments. Morphological properties are used to distinguish eggs from impurities and two features of the egg contents under brightfield and darkfield illumination are used in a statistical classification to distinguish eggs with undifferentiated contents (non-embryonated eggs) from eggs with fully developed larvae inside (embryonated eggs). Results: For assessment of the instrument[U+05F3]s performance, six egg suspensions of varying quality were used to generate a dataset of unseen images. Subsequently, annotation of the detected eggs and impurities revealed a high agreement with the manual, image-based assessments for both concentration and embryonation percentage (both error rates <1.0%). Similarly, a strong correlation was demonstrated in a final, blinded comparison with traditional microscopic assessments performed by an experienced laboratory technician. Conclusions: The present study demonstrates the applicability of computer vision in the production, analysis, and quality control of T. suis eggs used as an active pharmaceutical ingredient for the treatment of autoimmune diseases. © 2014 Elsevier Ltd.


Vejzagic N.,Copenhagen University | Thamsborg S.M.,Parasite Technologies A S | Thamsborg S.M.,Copenhagen University | Kringel H.,Parasite Technologies A S | And 3 more authors.
Parasitology Research | Year: 2015

Eggs of the pig whipworm, Trichuris suis ova (TSO), are currently tested in human clinical trials for their potential immunomodulatory capacity. The biological potency of TSO (egg viability and infectivity) is traditionally assessed in Göttingen minipigs as the establishment of intestinal larvae after inoculation with a known number of eggs. To minimize testing in animal models, development of an in vitro egg hatching assay is proposed as a reliable, cost-effective, and a faster alternative to test the egg viability. The present study aimed to investigate the influence of different chemical, physical, and biological factors on egg hatching. Thus, in a series of experiments and in different combinations, the eggs were stimulated with glass beads, artificial gastric juice, bile salt and trypsin solution, fermentation gut medium, or stimulated with mucosal scrapings from the ileum and the large intestine of the infected and uninfected Göttingen minipig. Mechanical stimulation with glass beads presented a simple and reproducible method for egg hatching. However, incubation of eggs with mucosal scrapings from the ileum, caecum, and colon for 24 h at 38 °C significantly increased hatching. © 2015, Springer-Verlag Berlin Heidelberg.


Vejzagic N.,Copenhagen University | Roepstorff A.,Parasite Technologies A S | Kringel H.,Parasite Technologies A S | Thamsborg S.M.,Parasite Technologies A S | And 3 more authors.
Veterinary Parasitology | Year: 2015

Embryonated eggs of the pig whipworm Trichuris suis (TSOee) constitute the active pharmaceutical ingredient (API) in a medicinal product explored in human clinical trials against several immune-mediated diseases. The measurement of TSO biological potency (hatchability and infectivity) is a requirement for the assessment of TSO's pharmacological potency in human clinical trials. The present study aims to validate the dose-dependent establishment of T. suis larvae in Göttingen minipigs and eventual clinical implication of a dose range (1000-10,000 TSO). Four groups of 5 minipigs were inoculated with doses of 1000, 2500, 7500, and 10,000 TSOee, respectively, to evaluate a range of concentrations of TSOee in a minipig infectivity model. Unembryonated eggs (TSOue) were added to keep the total egg number in the inoculum constant at 10,000 eggs. Two groups received 2500 and 7500 TSOee per pig without the addition of TSOue as controls. The intestinal larval establishment at 21 days post inoculation (dpi) demonstrated a clear positive linear dose-response relationship between numbers of inoculated TSOee and recovered larvae. There was a low level of variation in larval counts in all study groups. Thus, the infectivity model in minipigs within the tested dose range offers a reliable, sensitive and accurate assay for testing biological potency of TSO. © 2015 Elsevier B.V.


There is provided a computer vision based method for extracting features relating to the developmental stages of Trichuris spp. eggs, wherein for the final developmental stages a larva is present inside the egg, said Trichuris spp. eggs having a substantially oblong or elliptical shape with a protruding polar plug at each end, the shape of the Trichuris spp. eggs thereby defining a longitudinal direction and a transverse direction of the eggs.


PubMed | Parasite Technologies A S and Copenhagen University
Type: | Journal: Computers in biology and medicine | Year: 2014

OvaSpec is a new, fully automated, vision-based instrument for assessing the quantity (concentration) and quality (embryonation percentage) of Trichuris suis parasite eggs in liquid suspension. The eggs constitute the active pharmaceutical ingredient in a medicinal drug for the treatment of immune-mediated diseases such as Crohns disease, ulcerative colitis, and multiple sclerosis.This paper describes the development of an automated microscopy technology, including methodological challenges and design decisions of relevance for the future development of comparable vision-based instruments. Morphological properties are used to distinguish eggs from impurities and two features of the egg contents under brightfield and darkfield illumination are used in a statistical classification to distinguish eggs with undifferentiated contents (non-embryonated eggs) from eggs with fully developed larvae inside (embryonated eggs).For assessment of the instruments performance, six egg suspensions of varying quality were used to generate a dataset of unseen images. Subsequently, annotation of the detected eggs and impurities revealed a high agreement with the manual, image-based assessments for both concentration and embryonation percentage (both error rates <1.0%). Similarly, a strong correlation was demonstrated in a final, blinded comparison with traditional microscopic assessments performed by an experienced laboratory technician.The present study demonstrates the applicability of computer vision in the production, analysis, and quality control of T. suis eggs used as an active pharmaceutical ingredient for the treatment of autoimmune diseases.


PubMed | Parasite Technologies A S and Copenhagen University
Type: Journal Article | Journal: Veterinary parasitology | Year: 2015

Embryonated eggs of the pig whipworm Trichuris suis (TSOee) constitute the active pharmaceutical ingredient (API) in a medicinal product explored in human clinical trials against several immune-mediated diseases. The measurement of TSO biological potency (hatchability and infectivity) is a requirement for the assessment of TSOs pharmacological potency in human clinical trials. The present study aims to validate the dose-dependent establishment of T. suis larvae in Gttingen minipigs and eventual clinical implication of a dose range (1000-10,000 TSO). Four groups of 5 minipigs were inoculated with doses of 1000, 2500, 7500, and 10,000 TSOee, respectively, to evaluate a range of concentrations of TSOee in a minipig infectivity model. Unembryonated eggs (TSOue) were added to keep the total egg number in the inoculum constant at 10,000 eggs. Two groups received 2500 and 7500 TSOee per pig without the addition of TSOue as controls. The intestinal larval establishment at 21 days post inoculation (dpi) demonstrated a clear positive linear dose-response relationship between numbers of inoculated TSOee and recovered larvae. There was a low level of variation in larval counts in all study groups. Thus, the infectivity model in minipigs within the tested dose range offers a reliable, sensitive and accurate assay for testing biological potency of TSO.

Loading Parasite Technologies A S collaborators
Loading Parasite Technologies A S collaborators