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Tang D.-W.,Sun Yat Sen University | Lin G.-S.,Nanhai Branch of Foshan Public Security Bureau | Huang J.-L.,Sun Yat Sen University | Liu C.,China Institute of Technology | And 4 more authors.
Journal of Forensic Medicine | Year: 2012

Objective: To investigate the changes of collagen fibers and the expression of osteopontin in the left ventricle in cases of hypertrophic cardiomyopathy (HCM), along with the significance of their potential forensic application. Methods: Fifteen cases of HCM, 15 cases of coronary heart disease with cardiac hypertrophy and 20 cases of traffic accidents were selected as HCM group, coronary heart disease group and control group, respectively. Collagen volume fraction and osteopontin expression were observed and compared by HE staining, Masson trichrome staining and immunohistochemistry methods. Imaging and statistical methods were used for quantitative analysis. Results: Collagen volume fraction in left ventricle of HCM and coronary heart disease were significantly higher than that in the control group (P<0.05), which was not significantly different between the HCM group and the coronary heart disease group. The integral light density value of osteopontin in left ventricular cardiomyocytes of the HCM group and the coronary heart disease group were significantly higher than that of the control group (P<0.05), and the value of the HCM group was also significantly higher than that of coronary heart disease group (P<0.05). Conclusion: The increased contents of collagen fibers and the overexpression of osteopontin may play an important role in myocardial fibrosis, and they can be used as markers in aid of diagnosing sudden death due to HCM.


Hao B.,Sun Yat Sen University | Gao D.,Sun Yat Sen University | Tang D.-W.,Sun Yat Sen University | Wang X.-G.,Sun Yat Sen University | And 7 more authors.
Journal of Forensic Medicine | Year: 2012

To explore the mechanism that how human enterovirus 71(EV71) invades the brainstem and how intercellular adhesion molecules-1 (ICAM-1) participates by analyzing the expression and distribution of human EV71, and ICAM-1 in brainstem of infants with brain stem encephalitis. Methods: Twenty-two brainstem of infants with brain stem encephalitis were collected as the experimental group and 10 brainstems of fatal congenital heart disease were selected as the control group. The sections with perivascular cuffings were selected to observe EV71-VP1 expression by immunohistochemistry method and ICAM-1 expression was detected for the sections with EV71-VP1 positive expression. The staining image analysis and statistics analysis were performed. The experiment and control groups were compared. Results: (1) EV71-VP1 positive cells in the experimental group were mainly astrocytes in brainstem with nigger-brown particles, and the control group was negative. (2) ICAM-1 positive cells showed nigger-brown. The expression in inflammatory cells (around blood vessels of brain stem and in glial nodules) and gliocytes increased. The results showed statistical difference comparing with control group (P<0.05). Conclusion: The brainstem encephalitis can be used to diagnose fatal EV71 infection in infants. EV71 can invade the brainstem via hematogenous route. ICAM-1 may play an important role in the pathogenic process. © 2012 by the Editorial Department of Journal of Forensic Medicine.

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