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Guangzhou, China

Pu Z.-C.,Panyu Branch | Xie W.-Y.,Panyu Branch | Wang Y.-B.,Panyu Branch | Xue J.,Panyu Branch | Zhang X.-S.,Panyu Branch
Chinese Journal of Tissue Engineering Research | Year: 2015

BACKGROUND: Gene transfer techniques have been actively used in tissue regeneration therapy. Bone morphogenetic protein 7 with osteoinductive properties can effectively promote osteoblast growth and new bone formation. OBJECTIVE: To explore the influence of bone morphogenetic protein 7 adenovirus gene transfection on the biological function of bone marrow stromal stem cells. METHODS: Goat bone marrow stromal stem cells were isolated, cultured and transfected by recombinant adenovirus containing bone morphogenetic protein 7 (Adeno-BMP7). Cell ultrastructure was observed by transmission electronic microscope, cell cycle was detected by flow cytometry, expression of bone morphogenetic protein 7 was measure by western blot assay, and Von Kossa staining was used to observe the formation of calcium nodules. Bone marrow stromal stem cells transfected for 3 days and untransfected were used to prepare coral-cell complexes that were injected subcutaneously into the back of nude mice for 4 weeks and 8 weeks, followed by gross observation and histological examination. RESULTS AND CONCLUSION: Adeno-BMP7-transfected bone marrow stromal stem cells appeared to have active substance synthesis and metabolism. Adeno-BMP7 transfection played no effect on the cell cycle of bone marrow stromal stem cells. Bone morphogenetic protein 7 was expressed in the transfected bone marrow stromal stem cells. Larger calcium nodules were visible after Adeno-BMP7 transfection. Adeno-BMP7-transfected bone marrow stromal stem cells showed stronger osteogenic capability and higher bone quality. These results demonstrate that Adeno-BMP7 transfection can effectively promote the osteogenic differentiation of bone marrow stromal stem cells. © 2015 Journal of Clinical Rehabilitative Tissue Engineering Research. All rights reserved. Source

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