Tesfay L.,University of Connecticut Health Center |
Clausen K.A.,JK |
Kim J.W.,Panagene Inc. |
Hegde P.,University of Connecticut Health Center |
And 10 more authors.
Cancer Research | Year: 2015
Hepcidin is a circulating peptide hormone made by the liver that is a central regulator of systemic iron uptake and recycling. Here, we report that prostate epithelial cells also synthesize hepcidin, and that synthesis and secretion of hepcidin are markedly increased in prostate cancer cells and tissue. Prostatic hepcidin functions as an autocrine hormone, decreasing cell surface ferroportin, an iron exporter, increasing intracellular iron retention, and promoting prostate cancer cell survival. Synthesis of hepcidin in prostate cancer is controlled by a unique intersection of pathways that involves BMP4/7, IL6, Wnt, and the dual BMP and Wnt antagonist, SOSTDC1. Epigenetic silencing of SOSTDC1 through methylation is increased in prostate cancer and is associated with accelerated disease progression in patients with prostate cancer. These results establish a new connection between iron metabolism and prostate cancer, and suggest that prostatic dysregulation of hepcidin contributes to prostate cancer growth and progression. © 2015 AACR.
Panagene Inc. | Date: 2012-11-27
DNA chips; Optical glass for the manufacture of laboratory equipment; Waling glass in the nature of laboratory glassware; lens glass in the nature of eyeglass lenses; ultraviolet-ray transmitting glass in the nature of laboratory glassware; infrared-ray absorbing glass in the nature of laboratory glassware; glass covered with an electrical conductor for use in the manufacture of laboratory glassware; apparatus and instruments for physics, namely, polymerase chain reaction machine, image scanner, spectrophotometer, electrophoresis apparatus for use in laboratories, hybridization chamber, and microarray of biological probes; chemistry apparatus and instruments, namely, pH meter and chromatography apparatus for use in polymer synthesis and purification; and diffraction apparatus, namely, optical lenses and prisms for microscopes. Vaccines and pharmaceutical research and development; Research of geriatric diseases; Bacteriological research; Cancer research; Pharmaceutical development; pharmaceutical research; pharmaceutical testing in the field of pharmacology; chemical-pharmaceutical testing; Medical research, namely, research of medicine, research and development of DNA chips, research of biotechnology; scientific and technical consultation in the field of biotechnology; biological research; Chemical, biochemical, biological and bacteriological research and analysis, namely, analysis of biochemistry, analysis of DNA, and research of genetics; Laboratory apparatus rental; Research and development for others in the fields of microarray; rental of research installations in the nature of laboratory apparatus and instruments; chemistry services, namely, chemical analysis.
Kim J.H.,Korea Research Institute of Bioscience and Biotechnology |
Kim J.-W.,Panagene Inc. |
Chung B.H.,Korea Research Institute of Bioscience and Biotechnology
Journal of Colloid and Interface Science | Year: 2011
We report an enzymatic method to control the plasmon resonance absorbance of gold nanoparticle (AuNP) arrays assembled on hyaluronic acids. While multiple electrostatic interactions between cysteamine on the AuNPs and the carboxylic acid residues in the whole intact hyaluronic acid induced the formation of large aggregates, precise control of the plasmon absorbance was possible by tailoring the size of the bio-polymeric templates with hyaluronidase, almost over the entire range of the resonant coupling wavelengths. It was possible to precisely tune the position of the second plasmon absorbance by manipulating the amount of the template and the enzymatic hydrolysis time. Finally, we were able to produce a chain-like array of AuNPs, which was nearly one dimensional, with a maximum shift of up to 189. nm in the plasmon absorbance at the optimal hydrolysis time of the templates. This enzymatic method can be used as a useful tool to tailor the plasmonic properties of the nanostructures required for specific applications. © 2011 Elsevier Inc.
Yin H.,University of Oxford |
Yin H.,Tianjin Medical University |
Betts C.,University of Oxford |
Saleh A.F.,Medical Research Council Laboratory of Molecular Biology |
And 6 more authors.
Molecular Therapy | Year: 2010
Antisense oligonucleotides (AOs) have the capacity to alter the processing of pre-mRNA transcripts in order to correct the function of aberrant disease-related genes. Duchenne muscular dystrophy (DMD) is a fatal X-linked muscle degenerative disease that arises from mutations in the DMD gene leading to an absence of dystrophin protein. AOs have been shown to restore the expression of functional dystrophin via splice correction by intramuscular and systemic delivery in animal models of DMD and in DMD patients via intramuscular administration. Major challenges in developing this splice correction therapy are to optimize AO chemistry and to develop more effective systemic AO delivery. Peptide nucleic acid (PNA) AOs are an alternative AO chemistry with favorable in vivo biochemical properties and splice correcting abilities. Here, we show long-term splice correction of the DMD gene in mdx mice following intramuscular PNA delivery and effective splice correction in aged mdx mice. Further, we report detailed optimization of systemic PNA delivery dose regimens and PNA AO lengths to yield splice correction, with 25-mer PNA AOs providing the greatest splice correcting efficacy, restoring dystrophin protein in multiple peripheral muscle groups. PNA AOs therefore provide an attractive candidate AO chemistry for DMD exon skipping therapy. © The American Society of Gene and Cell Therapy.
Kim Y.-T.,Panagene Inc. |
Kim J.W.,Panagene Inc. |
Kim S.K.,Panagene Inc. |
Joe G.H.,Panagene Inc. |
Hong I.S.,Kongju National University
ChemBioChem | Year: 2015
It has been very difficult to detect and quantify multiple somatic mutations simultaneously in single-tube qPCR. Here, a novel method for simultaneous detection of multiple mutations and a melting curve analysis was developed by using clamping PNA and detection PNA probes. Each PNA probe was designed to have a specific melting temperature by the introduction of γ-PNA monomer. This technique was successfully applied to the detection of six genotypes in two different mutations of K-RAS at the same time. Such simultaneous analysis of an amplified curve and a melting curve in qPCR can be widely used for the early diagnosis of cancer and determining the prognosis of drug treatments. © 2015 Wiley-VCH Verlag GmbH & Co. KGaA.