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Palma Beez Research Institute Co. | Date: 2006-03-10

Diagnostic preparations for medical purposes; drugs for medical purposes, namely, in vitro diagnostic deals, namely, diagnotic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; in vitro diagnostic kits and reagents, namely, in vitro diagnostic test kits that can be used for the detection or analysis of DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplification comprised of reagents, assay buffer solutions, and negative control material for clinical and medical laboratory use. Chemistry apparatus and instruments, namely, sampler probes for scientific purposes, namely, probes used for collecting samples to detect or analyze DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplification. Diagnostic apparatus for medical purposes, namely, analytical apparatus used for the detection or analysis of DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplification.


Usui M.,PALMA BeeZ Research Institute Co. | Usui M.,Nihon University | Usui M.,EIDIA Co. | Fujikawa T.,PALMA BeeZ Research Institute Co. | And 13 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene β-actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs. PALSAR could detect mRNA directly without synthesizing cDNA. Moreover, multiple mRNAs could be detected simultaneously in a single reaction tube and there was a good correlation between the results obtained PALSAR and those by real-time PCR. © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license. Source


Trademark
Palma Beez Research; Institute Co. | Date: 2007-12-04

Diagnostic preparations for medical purposes; drugs for medical purposes, namely, invitro diagnostic deals, namely, diagnostic reagents for in vitro use in biochemistry, clinical chemistry and microbiology; in vitro diagnostic kits and reagents, namely, in vitro diagnostic test kit that can be used for the detection or analysis of DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplication comprised of reagents, assay buffer solutions, and negative control material for clinical and medical laboratory use. Chemistry apparatus and instruments, namely, sampler probes for scientific purposes, namely, probes used for collecting samples to detect or analyze DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplification. Diagnostic apparatus for medical purposes, namely, analytical apparatus used for the detection or analysis of DNA, RNA, proteins, viruses, microorganisms, infectious diseases, biochemical samples, and for signal amplification.

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