PALMA BeeZ Research Institute Co.

Japan

PALMA BeeZ Research Institute Co.

Japan

Time filter

Source Type

Usui M.,PALMA BeeZ Research Institute Co. | Usui M.,Nihon University | Usui M.,EIDIA Co. | Fujikawa T.,PALMA BeeZ Research Institute Co. | And 12 more authors.
Biochemical and Biophysical Research Communications | Year: 2015

We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene β-actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs. PALSAR could detect mRNA directly without synthesizing cDNA. Moreover, multiple mRNAs could be detected simultaneously in a single reaction tube and there was a good correlation between the results obtained PALSAR and those by real-time PCR. © 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.


PubMed | Japan National Institute of Infectious Diseases, PALMA BeeZ Research Institute Co. and Nihon University
Type: Journal Article | Journal: Biochemical and biophysical research communications | Year: 2015

We describe a novel technology for detecting nucleic acids: Probe Alteration Link Self-Assembly Reactions (PALSAR). PALSAR comprises DNA self-assembly of pairs of short DNA probes formed by alternate hybridization of three complementary regions in a pair of honeycomb probes (HCPs). Self-assembly occurs at designated salt concentrations and reaction temperatures and requires no enzymes. We prepared pairs of HCPs to detect mRNAs encoded by the GAPDH gene -actin (BA) gene, CD3D gene, CD4 gene, major vault protein (MV) gene and the signalling lymphocytic activation molecule-associated protein (SAP) gene, and succeeded in quantitatively detecting these mRNAs. PALSAR could detect mRNA directly without synthesizing cDNA. Moreover, multiple mRNAs could be detected simultaneously in a single reaction tube and there was a good correlation between the results obtained PALSAR and those by real-time PCR.

Loading PALMA BeeZ Research Institute Co. collaborators
Loading PALMA BeeZ Research Institute Co. collaborators