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Zholobak N.M.,Ukrainian Academy of Sciences | Popov A.L.,Institute of Theoretical and Experimental Physics | Shcherbakov A.B.,Ukrainian Academy of Sciences | Popova N.R.,Institute of Theoretical and Experimental Physics | And 7 more authors.
Beilstein Journal of Nanotechnology | Year: 2016

Luminescent organic dots (O-dots) were synthesized via a one-pot, solvent-free thermolysis of citric acid in urea melt. The influence of the ratio of the precursors and the duration of the process on the properties of the O-dots was established and a mechanism of their formation was hypothesized. The multicolour luminescence tunability and toxicity of synthesized O-dots were extensively studied. The possible applications of O-dots for alive/fixed cell staining and labelling of layer-by-layer polyelectrolyte microcapsules were evaluated. © 2016 Zholobak et al.


Chernyshenko V.,Palladin Institute of Biochemistry NAS of Ukraine | Platonova T.,Palladin Institute of Biochemistry NAS of Ukraine | Makogonenko Y.,Palladin Institute of Biochemistry NAS of Ukraine | Rebriev A.,Palladin Institute of Biochemistry NAS of Ukraine | And 3 more authors.
Biochimie | Year: 2014

The variety of enzymes including serine proteases that possess fibrin(ogen)olytic and platelet modulating activity have been discovered in different snake venoms. In our work the fibrin(ogen)olytic and platelet modulating activity of a new protease from Echis multisquamatis snake venom was studied. It was shown that purified enzyme cleaved the BβR42-A43 bond of fibrinogen during first contact with the substrate following much slower hydrolysis of C-terminus of fibrinogen Aα-chain. Protease hydrolysed fibrin clot too, but at much slower rate and cleaved both C-terminus of Aα-chain and BβR42-A43 bond of Bβ-chain simultaneously. Preincubation of fibrinogen with protease dramatically elongated thrombin clotting time and the clot formed from a mixture of native fibrinogen and fibrinogen desBβ(1-42)2 digested by plasmin much faster than a native fibrin clot. The protease did not activate platelets nor cause changes in their shape and granularity, but it reduced platelets aggregation induced by ADP. © 2014 Elsevier Masson SAS. All rights reserved.

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