Vemula S.K.,Jangaon Institute of Pharmaceutical science |
Veerareddy P.R.,Palamuru University
Drug Delivery and Translational Research | Year: 2014
Present research efforts are focused in developing compression-coated ketorolac tromethamine tablets to improve the drug levels in colon by retarding the drug release in the stomach and small intestine. To achieve this objective, core tablets containing ketorolac tromethamine were prepared by direct compression and compression coated with sodium alginate. The developed tablets were evaluated for physical properties, in vitro drug release, X-ray imaging, and pharmacokinetic studies in human volunteers. Based on the in vitro drug release study, the optimized formulation showed very little drug release (6.75 ± 0.49 %) in the initial lag period of 5 h, followed by progressive release up to 97.47 ± 0.93 % within 24 h. The X-ray imaging of tablets in human volunteers showed that the tablets reached the colon without disintegrating in the upper gastrointestinal tract. From the pharmacokinetic study, the C max of colon-targeted tablets was 3,486.70 ng/ml at T max 10 h, whereas in the case of immediate-release tablets, the C max of 4,506.31 ng/ml at T max 2 h signifies the ability of compression-coated tablets to target the colon. In conclusion, compression-coated tablets are suitable to deliver ketorolac tromethamine to the colon. © 2014 Controlled Release Society.
Satyanarayana S.D.V.,Koneru Lakshmaiah College of Engineering |
Krishna M.S.R.,Koneru Lakshmaiah College of Engineering |
Kumar P.P.,Palamuru University
3 Biotech | Year: 2017
Soil is major reservoir for microbes and harbors a vast microbial diversity. Soil microbiota plays a pivotal role in biogeochemical cycles, bioremediation, and in health and disease states of humans, animals, and plants. It is imperative to understand the microbial signatures which are specific in such an ecosystem to unravel their potential role and impact on environment. During the recent years, exploration of soil microbial communities has been geared up with the advent of advanced sequencing technologies. Introduction of custom-made protocols and optimized procedures have enhanced the accuracy levels along with cost-effectiveness of DNA extraction. Standardization of DNA extraction method from soil microbiota has its own limitations due to different nature of soils and the complexity of ecosystems. Though a few standardized protocols are in usage, huge variations and complexities among the microbial communities frequently suggest the optimization, based on various known and unknown factors. Therefore, a set of four standardized DNA isolation protocols was comparatively analyzed with respect to our custom-made protocol owing to the scientific fact that the same protocol does not hold good for all soil samples. Furthermore, the developed protocol has been successfully applied for the identification of efficient plant-specific Rhizobial stains for five legume plants from the soils of various locations under same geographical region. Out of 40 Badrachalam forest soils, five samples, KPFS36, CHFS17, TPFS33, GVFS06, and GPFS40, one for each of Arachis hypogaea, Vigna radiata, Vigna mungo, Glycine max, and Cicer arietinum plants, were selected, respectively, for the soil DNA extraction. A considerable improvement in the DNA yield was identified using the modified protocol with a yield of 21.08 μg/g providing abundant DNA fragments for further investigation on Rhizobial species. © 2017, Springer-Verlag Berlin Heidelberg.
Pragada R.R.,Andhra University |
Veeraredy P.R.,Palamuru University
Latin American Journal of Pharmacy | Year: 2015
The aim of the present investigation was to screen out the key variables that influence on selected dependent variables using a 5-factor, 12-run Plackett-Burman screening design (PBSD). Microspheres were fabricated by quasi-emulsion solvent diffusion method. The selected independent and dependent variables were amount of ketoprofen (X1), % of polyvinyl alcohol (X2), amount of eudragit S-100 (X3), temperature (X4) and paddle speed (X5); and Particle size (YPS), drug entrapment efficacy (YDEE), % cumulative drug release at 5th h (YQ5) and 10th h (YQ10), respectively. These microspheres were studied by SEM and FTIR. Quantitative evaluation of results showed that the X1, X3 and X5 were the key factors on YDEE. The main effects decreased in the order: X3 (+37.41) > X5 (-16.41) > X1 (-9.66) >X2 (-5.10) > X4 (-4.04). The PBSD is helpful in screen out the key variables for the development of pH sensitive ketoprofen colon specific microspheres. © 2015 Colegio de Farmaceuticos de la Provincia de Buenos Aires. All rights reserved.
Tharun G.,Palamuru University |
Pindi P.K.,Palamuru University
Journal of Pharmacy Research | Year: 2013
Background: An increasing demand for natural additives has shifted the attention from synthetic to natural antioxidants. As leafy vegetables are found to be good source of antioxidants and the present study is to examine the antioxidant potential and antimicrobial activity of leaf extracts of Pimpinella tirupatiensis. Methods: Antioxidant potential of leaves of P. tirupatiensis was studied using different methods like DPPH, nitric oxide, hydrogen peroxide scavenging activity. Reducing power and antimicrobial activity was estimated by using both gram positive and gram negative microorganisms by using DMF as solvent. Results: The aqueous extracts showed maximum scavenging activity of DPPH followed by nitric oxide, hydrogen peroxide and reducing power respectively. Benzene and alcoholic extract showed maximum antimicrobial activity. Conclusion: Substantial amounts of antioxidants including vitamins C and E, carotenoids, flavonoids, phenols and tannins etc. can be utilized to scavenge the excess free radicals from the body. © 2013 JPR Solutions.
Pindi P.K.,Palamuru University |
R. R.S.,Palamuru University |
Pavankumar T.L.,Palamuru University
Journal of Microbiology | Year: 2014
Metagenomics is a magnificent tool to isolate genes from unknown/uncharacterized species and also from organisms that cannot be cultured. In this study, we constructed a metagenomic library from isolated DNA of soil samples collected from Palamuru University campus premises, in Mahabubnagar district of Andhra Pradesh, India. We isolated a novel lipase gene LipHim1, which has an open reading frame of 591 base pairs and encodes ∼23 kDa protein consisting of 196 amino acids. The Lipase LipHim1 showed maximum 32% homology at the protein level with the extracellular Aeromonas hydrophila lipase (Class II, GDSL family) and was significantly different from all other known lipases. The isolated lipase catalyzed the hydrolysis of fatty acid esters of polyoxyethylene sorbitan such as Tween 60. Our results indicate that the isolated lipase gene is novel. © 2014 The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg.
Harish Kumar Reddy Y.,Osmania University |
Srijana M.,Osmania University |
Madhusudhan Reddy D.,Palamuru University |
Gopal R.,Osmania University
African Journal of Biotechnology | Year: 2010
Banana is a major cash crop of many regions generating good amount of waste after harvest. This agro waste which is left for natural degradation is used as substrate for single step ethanol fermentation by thermophilic, cellulolytic, ethanologenic Clostridium thermocellum CT2, a new culture isolated from elephant droppings. Scanning electron microscopic pictures clearly indicate cellulolysis and close interaction of selected isolate CT2 with cellulose. The optimum conditions for cellulose fermentation were 60°C, pH 7.5, inoculums size 5% and incubation time 5 days. Ethanol produced and reducing sugars were estimated by gas chromatography. Clostridium thermosaccharolyticum HG8 and Thermoanaerobacter ethanolicus ATCC 31937 were used in coculture fermentation with CT2. Coculture fermentation of CT2 with HG8 was more efficient in terms of ethanol production, cellulose degradation and reducing sugars utilization. A maximum ethanol yield of 0.41g/g substrate used was obtained on coculturing CT2 with HG8 on alkali treated banana waste. Coculture was active even at substrate concentrations up to 100 g/l, a maximum ethanol of 22 g/l was obtained at 100 g/l substrate concentration on coculturing CT2 with HG8. This is the first report on anaerobic single step conversion of banana waste to ethanol by C. thermocellum. © 2010 Academic Journals.
Kumar P.P.,Palamuru University
International Journal of Pharma and Bio Sciences | Year: 2011
Soil represents a diverse group of microorganism, which has frequently been used to isolate and explore and exploit microbes for industrial, environmental and agriculture applications. Presently, several methods are being used to isolate the DNA from environmental samples. This paper deals with an improved method developed for the isolation of heterologous DNA from environmental soils or sediments. This approach consists of the direct extraction of large fragmented nucleic acids from soil followed by purification. Cell lysis is a critical step in soil metagenomic DNA extraction. Extraction procedure was optimized with series of steps, which involved gentle mechanical lysis and number of freeze-thawing cycles in liquid nitrogen and with variations in incubation period and temperature. A comparison of the optimized protocol with other existing protocols and with commercially available kit suggests that protocol described in this report would be more efficient and yields high quantity and quality DNA from environmental samples.
Pindi P.K.,Palamuru University
African Journal of Microbiology Research | Year: 2011
An attempt was made to investigate the effect of different polluted soils on the mycorrhizal association, nodulation and subsequent growth of Acacia melanoxylon and Leucaena leucocephala tree species. Five industrial pollutant affected soils viz., Organic chemicals, pesticide, detergent, paper and neem seed oil cake were employed in this study and Palamuru University Campus soil used as a control. Among the five types of effluents, Adarsha Paper Industries and Vidyadhari neem extract effluents exerted less negative effect. Corey organics and Rhyme organics factory effluents have shown maximum inhibitory effect, K. K. Detergent factory effluents exerted moderate inhibitory effect. Both agro forestry tree species were shown similar response towards AM colonization, nodulation and growth against five industrial effluents. © 2011 Academic Journals.
Chinna Devi G.,Palamuru University |
Bhaskara Rao R.,Jawaharlal Nehru Technological University Anantapur
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2012
The objective of the study is to formulate and evaluate floating tablets of pioglitazone employing cross-linked starch-urea, a modified starch in comparison to HPMC K15 M, a synthetic cellulose derivative. Floating tablets of pioglitazone were prepared employing cross linked starch urea and HPMC K15 M as matrix formers, sodium bicarbonate as gas generating agent and bees wax and ethyl cellulose as floating enhancers and the tablets were evaluated for in vitro buoyancy and drug release characteristics. The floating characteristics of the formulations which contained sodium bicarbonate (15%) alone were not satisfactory with both the two polymers and need to be improved. Increasing the strength of sodium bicarbonate from 15% to 20% has not much improved the floating characteristics. Addition of beeswax (15%) and ethyl cellulose (5%) has significantly enhanced the buoyancy of the tablets formulated with both the two polymers. Pioglitazone release from the floating tablets prepared was slow and spread over 24 h and depended on the polymer used and composition of the tablets. Drug release was diffusion controlled and followed zero order kinetics. Non-Fickian diffusion was the drug release mechanism from all the tablets formulated. Pioglitazone release from the tablets containing beeswax and ethyl cellulose along with the matrix forming polymers was slow and spread over more than 24 h. The T90 values were in the range 19 - 24 h with these tablets. These tablets exhibited a floating time of 44 h after a floating lag time in the range 2-6 min. Cross-linked starch-urea is found suitable as matrix former for floating tablets and is comparable to HPMC K15M, a widely used polymer for floating tablets and for controlled release.
Pindi P.K.,Palamuru University
Asian Journal of Microbiology, Biotechnology and Environmental Sciences | Year: 2011
Gliricidia is a small genus of trees and shrubs and native of tropical America. G. maculata is widely cultivated in the tropics as a shade and ornamental tree. Studies on influence of bioinoculants on growth of Gliricidia maculata tree species responded positively towards the different inoculations. However, the response varied with the type of bioinoculant. Dual inoculations of mycorrhizal fungi and Rhizobium have shown better results than the other types. Dual inoculation involving mycorrhizae and Azotobacter, Rhizobium and PSB performed better than single inoculations of mycorrhizae. A mixture of mycorrhizae appears to be better inoculum than the individual AM fungi. AM fungal species varied in their efficiency in supporting the seedling growth. They further suggested that mixed inoculum might be used more efficiently than single inoculum for field inoculations. © Global Science Publications.