Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex

Peshāwar, Pakistan

Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex

Peshāwar, Pakistan

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Ahmad N.,Quaid-i-Azam University | Fazal H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | Abbasi B.H.,Quaid-i-Azam University | Farooq S.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex
International Journal of Phytomedicine | Year: 2010

Free radical scavenging activity of three important plants Ginkgo biloba, Stevia rebaudiana and Parthenium hysterophorous was carried out to evaluate and explore new potential sources of natural antioxidants. For this purpose the leaves of the three plants were processed. In these experiments the order of the antioxidant activity was, maximum activity shown by methanolic extract of Ginkgo biloba followed by Parthenium hysterophorous and Stevia rebaudiana. Furthermore the ethanolic extract of Ginkgo biloba also showed maximum antioxidant activity seconded by Stevia rebaudiana and Parthenium hysterophorous. © arjournals.org, All rights reserved.


Ahmad N.,Quaid-i-Azam University | Ahmad N.,Nuclear Institute for Food and Agriculture NIFA Tarnab | Fazal H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | And 4 more authors.
Asian Pacific Journal of Tropical Biomedicine | Year: 2011

The main objective of the current study is to investigate the potential of Carica papaya leaves extracts against Dengue fever in 45 year old patient bitten by carrier mosquitoes. For the treatment of Dengue fever the extract was prepared in water. 25 mL of aqueous extract of C. papaya leaves was administered to patient infected with Dengue fever twice daily i.e. morning and evening for five consecutive days. Before the extract administration the blood samples from patient were analyzed. Platelets count (PLT), White Blood Cells (WBC) and Neutrophils (NEUT) decreased from 176 × 103/μL, 8.10 × 103/μL, 84.0% to 55 × 103/μL, 3.7 × 103/μL and 46.0%. Subsequently, the blood samples were rechecked after the administration of leaves extract. It was observed that the PLT count increased from 55 × 103/μL to 168 × 103/μL, WBC from 3.7 × 103/μL to 7.7 × 103/μL and NEUT from 46.0% to 78.3%. From the patient feelings and blood reports it showed that Carica papaya leaves aqueous extract exhibited potential activity against Dengue fever. Furthermore, the different parts of this valuable specie can be further used as a strong natural candidate against viral diseases. © 2011 Asian Pacific Tropical Biomedical Magazine.


Imran M.,University of Peshawar | Khan H.,University of Peshawar | Shah M.,University of Peshawar | Khan R.,University of Peshawar | Khan F.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex
Journal of Zhejiang University: Science B | Year: 2010

In the present work, the fruits of four Morus species, namely Morus alba (white mulberry), Morus nigra (black mulberry), Morus laevigata (large white fruit), and Morus laevigata (large black fruit), were analyzed for proximate composition, essential minerals, and antioxidant potentials. For this purpose, the ripe fruits were collected from the northern regions of Pakistan. The major nutritional components (moisture, ash, lipids, proteins, fibres, carbohydrates, and total sugar) were found to be in the suitable range along with good computed energy. Total dry weight, pH, and titratable acidity (percent citric acid) were (17.60±1.94)-(21.97±2.34) mg/100 g, (3.20±0.07)-(4. 78±0.15), and (0.84±0.40)%-(2.00±0.08)%, respectively. Low riboflavin (vitamin B2) and niacin (vitamin B3) contents were recorded in all the fruits, while ascorbic acid (vitamin C) was in the range from (15.20±1.25) to (17.03±1.71) mg/100 g fresh weight (FW). The mulberry fruits were rich with regard to the total phenol and alkaloid contents, having values of(880±7.20)-(1650±12.25) mg/100 g FW and (390±3.22)- (660±5.25) mg/100 g FW, respectively. Sufficient quantities of essential macro-(K, Ca, Mg, and Na) and micro-(Fe, Zn, and Ni) elements were found in all the fruits. K was the predominant element with concentration ranging from (1270±9.36) to (1731±11.50) mg/100 g, while Ca, Na, and Mg contents were (440±3.21)-(576±7.37), (260±3.86)- (280±3.50), and (240±3.51)-(360±4.20) mg/100 g, respectivly. The decreasing order of micro-minerals was Fe>Zn>Ni. The radical scavenging activity of methanolic extract of fruits was concentration-dependent and showed a correlation with total phenolic constituents of the respective fruits. Based on the results obtained, mulberry fruits were found to serve as a potential source of food diet and natural antioxidants. © Zhejiang University and Springer-Verlag Berlin Heidelberg 2010.


Abbasi B.H.,Quaid-i-Azam University | Ahmad N.,Quaid-i-Azam University | Fazal H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | Mahmood T.,Quaid-i-Azam University
Journal of Medicinal Plants Research | Year: 2010

Piper nigrum, commonly known as "Black-pepper", has gained a global consideration because of its volume in the spice industry. This plant has shown great potential for the discovery of novel biologically active compounds and need for techniques to enhance the production of high quality consistent plant material for feasible accumulation of metabolites. Tissue culture of P. nigrum can play a vital role in germplasm conservation, enhanced multiplication and genetic engineering for feasible production of biologically active compounds. Liquid culture is yet to be established and reserves corner for industrial production of these active components. This review provides the developments in the propagation practices and challenges that remain in P. nigrum biotechnology. © 2010 Academic Journals.


Iqbal M.,University of Agriculture at Faisalabad | Iqbal M.,University of Peshawar | Bhatti I.A.,University of Agriculture at Faisalabad | Zia-Ur-Rehman M.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | And 2 more authors.
Asian Journal of Chemistry | Year: 2014

The possibility of applying photo-catalytic treatment (UV/H 2O2/TiO2) to reduce the cytotoxicity and mutagenicity as well as the microbial load of soap and detergent wastewater has been investigated. Wastewater from three soap and detergent industries were collected from Faisalabad, Gujranwala and Sargodha regions of Pakistan. Bioassays such as allium cepa, haemolytic and brine shrimp were utilized to assess the cytotoxicity, while mutagenicity was evaluated through Ames test. The microbial load; bacterial count and total coliform tests were performed under standardized conditions. Along with toxicity appraisal, the water quality parameter such as BOD, COD, DO, pH, TDS and TSS were also measured before and after application of UV/H2O2/TiO2 treatment. A considerable reduction in cytotoxicity and mutagenicity was achieved with the maximum degradation of pollutants. With the application of advanced oxidation treatment complete removal of cytotoxicity and mutagenicity was obtained and results indicate that the advanced oxidation process can be successfully used for toxicity removal, for the improvement of water quality parameters and hence the treated wastewater could possibly be used for irrigation purposes.


Ahmad N.,Quaid-i-Azam University | Fazal H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | Abbasi B.H.,Quaid-i-Azam University | And 3 more authors.
Plant Cell, Tissue and Organ Culture | Year: 2010

The organogenic potential and antioxidant potential (1, 1-diphenyl-2-picrylhydrazyl-scavenging activity) of the medicinal plant Piper nigrum L. (black pepper) were investigated. Callus induction and shoot regeneration were induced from leaf explants of potted plants cultured on MS medium supplemented with different plant growth regulators. The best callogenic response was observed on explants cultured for 30 days on MS medium supplemented with either 0.5 or 1.5 mg l -1 6-benzyladenine (BA) + 1.0 mg l -1 α-naphthaleneacetic acid. Subsequent transfer of the callogenic explants onto MS medium supplemented with 1.5 mg l -1 BA + 1.0 mg l -1 gibberellic acid (GA 3) achieved 85% shoot organogenesis after 30 days of culture. The maximum number (7.2) of shoots/explant was recorded for explants cultured in MS medium supplemented with 1.0 mg l -1 BA. Following the transfer of shoots to an elongation medium, the longest shoots (5.4 cm) were observed on MS medium supplemented with 1.0 mg l -1 BA + 1.0 mg l -1 GA 3. The elongated shoots were rooted on MS medium supplemented with different concentrations of indole butyric acid. An assay of the antioxidant potential of the in vitro-grown tissues revealed that the antioxidant activity of the regenerated shoots was significantly higher than that of callus and the regenerated plantlets. © 2010 Springer Science+Business Media B.V.


Ahmad N.,Quaid-i-Azam University | Abbasi B.H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex | Khan M.A.,Quaid-i-Azam University | Afridi M.S.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex
Comptes Rendus - Biologies | Year: 2014

In this study, a novel approach for in vitro regeneration of Piper nigrum L. has been applied in order to increase healthy biomass, phytochemicals and piperine production via reverse photoperiod (16hD/8hL). Leaf portions of the seed-derived plants were placed on an MS-medium fortified with different PGRs. Under 16hD/8hL, thidiazuron (TDZ; 4.0 mg L-1) and BA (1.5 mg L -1) was found to be the most effective (< 90%) in callus induction. Two concentrations (1.5, 2.0 mg L-1) of the IBA produced > 80% shoots from callus cultures. Healthy shoots were transferred to rooting medium and higher percentage of rooting (< 90%) was observed on IBA (1.5 mg L-1). These in vitro tissues were subjected to amino acid analysis, spectrophotometry, and HPLC. ARG, SER, THR, and TYR were the most abundant components out of 17 amino acids. Higher amino acid production was observed under normal photoperiod (16hL/8hD) than under reverse photoperiod (16hD/8hL). The highest total phenolic content (TPC; 9.91 mg/g-DW) and flavonoid content (7.38 mg/g-DW) were observed in callus cultures incubated under 16hL/8hD than other tissues incubated under 16hD/8hL photoperiod. Higher DPPH and PoMo activities were observed in tissues incubated under 16hL/8hD photoperiod, while ABTS and Fe2+ chelating activities were found higher in tissues incubated under reverse photoperiod. Significant quantities of piperine content were observed in all tissues except callus cultures. These results suggest that reverse photoperiod is a promising approach for callus induction, phytochemicals and piperine production for commercial applications. © 2013 Académie des sciences.


Ahmad N.,Quaid-i-Azam University | Abbasi B.H.,Quaid-i-Azam University | Fazal H.,Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex
Industrial Crops and Products | Year: 2013

Silybum (S.) marianum L. is medicinally important plant species from Asteraceae family. S. marianum have multiple industrial applications and its annual demand varies from 18 to 20. tons/annum. It is renowned for production of important antioxidants which are used for the regeneration of damaged hepatic cells. In this study we have evaluated antioxidant activity and its relation with plant development. Seeds were germinated under controlled conditions and DPPH-scavenging activity (DSA) was evaluated from 10 to 100 days in these seed derived plants. However, maximum DSA in leaves (60%) and intact plants (65.43%) was observed in 80 days old plants. The effect of different radiation doses (gamma; 5-20. kGy) on DSA (%) was also investigated. The treated (20. kGy) seeds germinated in soil and on MS-medium showed 45.43% and 59.26% DSA, respectively. However, untreated seed-derived plants showed 44.53% DSA. Silymarin was extracted from seeds and maximum activity (80.75%) was observed in higher concentrations (250. μg/ml ethanol). This study suggested that not only the seeds but the whole plants of S. marianum can be used for the protection of liver from toxins and infections. © 2013 Elsevier B.V..


PubMed | University of Swat, Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex and Quaid-i-Azam University
Type: | Journal: Journal of photochemistry and photobiology. B, Biology | Year: 2016

Light is one of the key elicitors that directly fluctuates plant developmental processes and biosynthesis of secondary metabolites. In this study, the effects of various spectral lights on biomass accumulation and production of antioxidant secondary metabolites in callus cultures of Prunella vulgaris were investigated. Among different spectral lights, green light induced the maximum callogenic response (95%). Enhanced fresh biomass accumulation was observed in log phases on day-35, when callus cultures were exposed to yellow and violet lights. Yellow light induced maximum biomass accumulation (3.67g/100ml) from leaf explants as compared to control (1.27g/100ml). In contrast, violet lights enhanced biomass accumulation (3.49g/100ml) from petiole explant. Maximum total phenolics content (TPC; 23.9mg/g-DW) and total flavonoids content (TFC; 1.65mg/g-DW) were observed when cultures were grown under blue lights. In contrast, green and yellow lights enhanced total phenolics production (TPP; 112.52g/100ml) and total flavonoids production (TFP; 9.64g/100ml) as compared to control. The calli grown under green, red and blue lights enhanced DPPH-free radical scavenging activity (DFRSA; 91.3%, 93.1% and 93%) than control (56.44%) respectively. The DFRSA was correlated either with TPC and TFC or TPP and TFP. Furthermore, yellow lights enhanced superoxide dismutase (SOD), peroxidase (POD) and protease activities, however, the content of total protein (CTP) was higher in control cultures (186g BSAE/mg FW) as compared to spectral lights. These results suggest that the exposure of callus cultures to various spectral lights have shown a key role in biomass accumulation and production of antioxidant secondary metabolites.


PubMed | University of Agriculture, Peshawar and Pakistan Council of Scientific and Industrial Research PCSIR Laboratories Complex
Type: Journal Article | Journal: Molecules (Basel, Switzerland) | Year: 2015

The influence of carboxy methyl cellulose (CMC) and guargum-based coatings containing silver nanoparticles was studied on the postharvest storage stability of the kinnow mandarin (Citrus reticulata cv. Blanco) for a period of 120 days (85%-90% relative humidity) at 4 C and 10 C. Physicochemical and microbiological qualities were monitored after every 15 days of storage. Overall results revealed an increase in total soluble solid (TSS), total sugars, reducing sugars and weight loss but this increase was comparatively less significant in coated fruits stored at 4 C. Ascorbic acid, total phenolics, and antioxidant activity was significantly enhanced in coated fruits stored at 4 C. Titratable acidity significantly decreased during storage except for coated kinnow stored at 4 C. In control samples stored at 10 C, high intensity of fruit rotting and no chilling injury was observed. Total aerobic psychrotrophic bacteria and yeast and molds were noticed in all treatments during storage but the growth was not significant in coated fruits at 4 C. Kinnow fruit can be kept in good quality after coating for four months at 4 C and for 2 months at 10 C.

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