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Jebali A.,University of Yazd | Jebali A.,Pajoohesh Laboratory | Hekmatimoghaddam S.,University of Yazd | Kazemi B.,Shahid Beheshti University of Medical Sciences | And 2 more authors.
IET Nanobiotechnology

The aim of this study was to evaluate four immunoassays, based on amine-modified bovine serum albumin nanoparticles (AMBSANPs). First, the capability of nitrate absorption by AMBSANPs under different conditions was evaluated. Then, serial concentrations of pure ßHCG were added to wells coated with ßHCG antibody for immunoassays 1 and 2, and wells coated with ßHCG aptamer for immunoassays 3 and 4. Next, AMBSANPs conjugated with ßHCG antibody was added for immunoassays 1 and 3, and AMBSANPs conjugated with ßHCG aptamer were added for immunoassays 2 and 4. Finally, the optical density (OD) of each well was read at 340 nm, and compared with controls. Moreover, the concentration of ßHCG in the clinical samples was quantified by immunoassays 1, 2, 3, 4 and ELISA, and then compared. The effect of some serum interferences on these immunoassay methods was evaluated. The authors observed that the amount of nitrate absorption by AMBSANPs increased with an increase in H + ion concentration and temperature, and decreased with an increase in ion strength. The correlation (R2) between ELISA and immunoassays 1, 2, 3 and 4 were 0.97, 0.97, 0.98, 0.99, respectively. It was found that the increase in the serum interferences led to a decrease in the measured ßHCG concentration. © The Institution of Engineering and Technology 2015. Source

Bafghi A.F.,University of Yazd | Daghighi M.,Pajoohesh Laboratory | Daliri K.,Pajoohesh Laboratory | Jebali A.,University of Yazd
Colloids and Surfaces B: Biointerfaces

The aim of this study was to investigate the effect of magnesium oxide nanoparticles (MgO NPs) and MgO NPs coated with glucose (MONPCG) on Leishmania (. L) major. First, the promastigotes of L. major were separately incubated with serial concentrations of MgO NPs and MONPCG for 24, 48, and 72. h at 37. °C. Then, the cell viability of promastigotes was evaluated by MTT assay. On the other hand, the relative expression of Cpb and GP63 genes was detected by quantitative-real time PCR. Based on results, the increase of concentration, both MgO NPs and MONPCG, and incubation time led to decrease of cell viability. Moreover, the expression of Cpb and GP63 genes was decreased with increase of concentration of MgO NPs and MONPCG. Also, the increase of incubation time led to decrease of their expression in MgO NPs treated promastogotes. But, in case of MONPCG treated promastogotes, the increase of incubation time did not change the expression of Cpb and GP63. Interestingly, MONPCG could silence Cpb and GP63 genes better than MgO NPs. Note, the capability was also seen at sub-toxic concentrations of MONPCG. © 2015 Elsevier B.V. Source

Jafary R.,University of Yazd | Khajeh Mehrizi M.,University of Yazd | Hekmatimoghaddam S.,University of Yazd | Jebali A.,Pajoohesh Laboratory
Journal of the Textile Institute

People’s inclination toward medical textiles for healthy life style has created a rapidly increasing market for antimicrobial textiles, which, in turn, has stimulated intensive research and development. The aim of our study was to prepare cellulose fabrics finished by allicin-conjugated nanocellulose, whose properties were investigated by scanning electron microscopy, X-ray diffraction, and Fourier transforms infrared spectroscopy. The antibacterial ability of the treated fabrics was determined by AATCC test method 100–1993. The durability of antimicrobial activity to washing process was also evaluated. The results showed significant antibacterial activity against Staphylococcus aurous, statistically different from negative control fabric (finished by nanocellulose without allicin) (p < 0.05). The antibacterial activity after two home laundering cycles of all finished fabrics was only slightly reduced. It can be concluded that allicin-conjugated nanocellulose can be attached to cellulose textiles by a simple conjugation method to create durable antibacterial properties. © 2014, © 2014 The Textile Institute. Source

Bafghi A.F.,University of Yazd | Jebali A.,University of Yazd | Daliri K.,Pajoohesh Laboratory
Colloids and Surfaces B: Biointerfaces

The main aim of this study was to evaluate the capability of silica nanowire conjugated with loop-shaped oligonucleotides (SNWCLSOs) to silence cysteine proteinase b (Cpb) gene in Leishmania (L) tropica. On the other hand, its toxicity on amastigotes and mouse peritoneal macrophages was evaluated by 5-diphenyl-tetrazolium bromide (MTT) assay. For control, two loop-shaped oligonucleotides (LSO) were considered. LSO1 and LSO2 were 5[U+02B9]-NH2-cccccaaaaaaaaaaaaaaaaaaaaaaaaaggggg-COOH-3[U+02B9] and LSO2: 5[U+02B9]-NH2-cccccttttttttttttttttttttttttttttttttttttttggggg-COOH-3[U+02B9], respectively. After 72h incubation at 37°C, AMSNW, LSO1, and LSO2 had no remarkable toxicity on L. tropica amastigote (2×105/mL) and mouse peritoneal macrophages (2×105/mL). In case of SNWCLSOs, they had high toxicity on L. tropica amastigote, but they had no effect on mouse peritoneal macrophages. At concentrations of 1, 10, and 25μg/mL, AMSNW, LSO1 and LSO2 had no effect on the gene expression. But, at concentration of 50 and 100μg/mL, decrease of gene expression was observed. In case of SNWCLSOs, they could dramatically decrease the gene expression. It could be concluded that since SNWCLSOs could silence Cpb gene with no remarkable toxicity, they are good choice for treat cutaneous leishmaniasis in future. As a new agent, it must be checked in vivo. © 2015 Elsevier B.V. Source

Jebali A.,Pajoohesh Laboratory | Hekmatimoghaddam S.,University of Yazd | Behzadi A.,University of Yazd | Rezapor I.,University of Yazd | And 11 more authors.

In this study, cellulose nanoparticles were prepared by acid hydrolysis, separately conjugated with allicin and lysozyme by a carbodiimide cross-linker, and characterized by scanning electron microscopy, dynamic light scattering, and Fourier transform infrared spectroscopy. Then, their antimicrobial properties were evaluated by the microdilution method and compared with allicin, lysozyme, and nanocellulose alone. The results showed that nanocellulose had few antimicrobial activities, but allicin-conjugated nanocellulose (ACNC) and lysozyme-conjugated nanocellulose (LCNC) had good antifungal and antibacterial effects against standard strains of Candida albicans, Aspergillus niger, Staphylococcus aureus, and Escherichia coli. Noticeably, although allicin and lysozyme had different minimum inhibitory concentrations (MICs) against all strains, the same quantity of MIC50 and MIC90 was observed for both ACNC and LCNC. The authors suggest that both ACNC and LCNC can be used in industries as an antimicrobial agent in food packaging, inside foodstuffs, and in textile materials. © 2013 Springer Science+Business Media Dordrecht. Source

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