Ahmadnagar, India
Ahmadnagar, India

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Kuchekar S.R.,Padmashri Vikhe Patil College | Kundlik M.L.,Padmashri Vikhe Patil College
Journal of Saudi Chemical Society | Year: 2011

A high-throughout bioanalytical method based on a solid-phase extraction (SPE), and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated for the quantification of mirtazapine (MRT) and desmethyl mirtazapine (DMRT) in heparinized human plasma. Plasma samples, without a drying and reconstitution step, were extracted by a simple SPE. The analytes and imipramine (internal standard, IS) chromatographed on a Betasil-C18 column. The total chromatographic run time was 1.8. min per sample. Response was a linear function of concentration in the range 0.1-100.0. ng/ml, with correlation coefficient ≥0.9994. The assay has excellent characteristics and has been successfully applied to bioequivalence study samples for estimation of MRT and DMRT in healthy human subjects. © 2010.


Kundlik M.L.,Padmashri Vikhe Patil College | Kuchekar S.R.,Padmashri Vikhe Patil College
Journal of Chromatographic Science | Year: 2012

A rapid liquid chromatographic method with electrospray ionization tandem mass spectrometric (LCMSMS) detection is developed and validated for quantification of glimepiride in heparinized human plasma. Plasma samples, without a drying and reconstitution step, are extracted by solid-phase extraction (SPE) and eluted with 0.9 mL of acetonitrilemethanol (1:1, v/v) containing 0.05 formic acid. The analyte and glimepiride d8 (internal standard, IS) are chromatographed on a C18 column; the mobile phase is acetonitrile2 mm ammonium formate (88:12, v/v), with the pH adjusted to 3.5 with formic acid, at a flow rate of 0.5 mL/min. The retention times of glimepiride and the IS are 0.93 min, and the runtime is 1.6 min per sample. Selected reaction monitoring of MH+ at m/z 491.20 and 499.26 result in stable fragment ions with m/z 351.80 and 359.96 for glimepiride and the IS, respectively. The response was a linear function of the concentration in the range of 2.0650.0 ng/mL, with r < 0.9994. The recovery of glimepiride and the IS ranged from 81.91 to 83.36. The assay has excellent characteristics and has been successfully used for the analysis of glimepiride in healthy human subjects in a bioequivalence study. It was well suited to clinical studies of the drug involving large numbers of samples. © 2011 The Author.


Gholap S.S.,Padmashri Vikhe Patil College
European Journal of Medicinal Chemistry | Year: 2016

Pyrrole derivatives comprise a class of biologically active heterocyclic compounds which can serve as promising scaffolds for antimicrobial, antiviral, antimalarial, antitubercular, anti-inflammatory and enzyme inhibiting drugs. Due to their inimitable anticancer and anti-tubercular properties, researchers were inspired to develop novel pyrrole derivatives for the treatment of MDR pathogens. In the present review the main target is to focus on the development of pyrrole mimics, with emphasis based on their structure activity relationship (SAR). The present review is being obliging for the future development of pyrrole therapeutics. © 2015 Elsevier Masson SAS.


Gopal Reddy P.,Padmashri Vikhe Patil College | Tathe P.,Padmashri Vikhe Patil College
Biochemical and Cellular Archives | Year: 2012

The objective of the study was to evaluate the effect of different concentrations of aqueous as well as ethanolic leaf extracts of W. fruticosa on the growth of five bacteria viz., Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa and Salmonella typhimurium. Both extracts inhibited the bacterial growth however leaves extracted in alcohol exhibited greater antibacterial activity. Comparatively K. pneumoniae is inhibited more while S. typhimurium and P. aeruginosa less.


Kundlik M.L.,Padmashri Vikhe Patil College | Zaware B.H.,Commerce and Science College | Kuchekar S.R.,Padmashri Vikhe Patil College
Bulgarian Chemical Communications | Year: 2010

A high-throughout bioanalytical method based on a solid phase extraction (SPE) and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis has been developed and validated for the quantification of tamosulosin in heparinzed human plasma. Plasma samples, without a drying and reconstitution step, were extracted by a simple SPE. The analytes and tamosulosin D4 isotope (internal standard, IS) were chromatographed on a Betabasic-8 column. The total chromatographic run time was 1.8 min per sample. The response was a linear function of concentration in the range of 0.075-50.0 ng/ml, with correlation coefficient ≥0.9992. The assay has excellent characteristics and was successfully applied to bioequivalence study samples for estimation of tamosulosin in healthy human subjects. © 2010 Bulgarian Academy of Sciences, Union of Chemists in Bulgaria.


Dhakane V.D.,University of Solapur | Gholap S.S.,Padmashri Vikhe Patil College | Deshmukh U.P.,Padmashri Vikhe Patil College | Chavan H.V.,University of Mumbai | Bandgar B.P.,University of Solapur
Comptes Rendus Chimie | Year: 2014

An efficient and convenient synthesis of 1,3-oxazine derivatives has been achieved by the one-pot, multicomponent condensation of α- or β-naphthol, an aniline and formaldehyde using thiamine hydrochloride (VB1) as a versatile biodegradable and reusable catalyst in water as a universal solvent.


Gholap S.,Padmashri Vikhe Patil College | Gunjal N.,Padmashri Vikhe Patil College
Arabian Journal of Chemistry | Year: 2013

An efficient 2,4,6-trichloro-1,3,5-triazine (TCT) mediated synthesis of N-benzoylthiourea derivatives from carboxylic acid has been described. The reaction of TCT (1), triethyl amine in dichloromethane gives tris-quaternary ammonium salt (A), reacted with carboxylic acid to form activated ester as an intermediate (B). Aroylthiocyanate was formed by the reaction of activated ester 'B' and ammonium isothiocyanate followed by aliphatic or aromatic amines affording structurally diverse N-benzoylthiourea derivatives 3. The synthesized compounds were characterized by IR, 1H NMR and mass spectral data. © 2013 King Saud University.


PubMed | Padmashri Vikhe Patil College
Type: | Journal: European journal of medicinal chemistry | Year: 2016

Pyrrole derivatives comprise a class of biologically active heterocyclic compounds which can serve as promising scaffolds for antimicrobial, antiviral, antimalarial, antitubercular, anti-inflammatory and enzyme inhibiting drugs. Due to their inimitable anticancer and anti-tubercular properties, researchers were inspired to develop novel pyrrole derivatives for the treatment of MDR pathogens. In the present review the main target is to focus on the development of pyrrole mimics, with emphasis based on their structure activity relationship (SAR). The present review is being obliging for the future development of pyrrole therapeutics.


PubMed | Padmashri Vikhe Patil College
Type: Journal Article | Journal: Journal of chromatographic science | Year: 2012

A rapid liquid chromatographic method with electrospray ionization tandem mass spectrometric (LC-MS-MS) detection is developed and validated for quantification of glimepiride in heparinized human plasma. Plasma samples, without a drying and reconstitution step, are extracted by solid-phase extraction (SPE) and eluted with 0.9 mL of acetonitrile-methanol (1:1, v/v) containing 0.05% formic acid. The analyte and glimepiride d8 (internal standard, IS) are chromatographed on a C(18) column; the mobile phase is acetonitrile-2 mm ammonium formate (88:12, v/v), with the pH adjusted to 3.5 with formic acid, at a flow rate of 0.5 mL/min. The retention times of glimepiride and the IS are 0.93 min, and the runtime is 1.6 min per sample. Selected reaction monitoring of MH(+) at m/z 491.20 and 499.26 result in stable fragment ions with m/z 351.80 and 359.96 for glimepiride and the IS, respectively. The response was a linear function of the concentration in the range of 2.0-650.0 ng/mL, with r 0.9994. The recovery of glimepiride and the IS ranged from 81.91 to 83.36%. The assay has excellent characteristics and has been successfully used for the analysis of glimepiride in healthy human subjects in a bioequivalence study. It was well suited to clinical studies of the drug involving large numbers of samples.


A rapid liquid chromatography coupled with ESI tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of topiramate in heparinized human plasma. The plasma samples were prepared by solid-phase extraction (SPE) method without drying and then reconstitution. Topiramate and the topiramate d-12 internal standard (IS) were chromatographed on a Betasil C18 column at a flow rate of 0.5mL/min. The total run time was 1.80min. An ESI interface with negative ion mode was selected for ionization of analyte and IS. The mass transition [M-H] ions used for detection were m/z 338.1078.20 for topiramate, m/z 350.4090.10 for IS. The method was linear in the concentration range of 10-4200ng/mL with r0.9992. Recovery of topiramate and IS ranged from 78.20 to 87.74%. The validated method has been successfully used to analyze human plasma samples for application in 100mg fasted and fed pharmacokinetic studies.

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