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Kumar P.M.,Padmashree Institute of Management and Sciences | Venkataranganna M.V.,Connexios Life science Pvt Ltd | Manjunath K.,Bangalore University | Ashok G.,Radiant Research Services Pvt Ltd.
Chinese Journal of Natural Medicines | Year: 2014

The present study was undertaken to evaluate the influence of the methanolic fruit extract of Momordica cymbalaria (MFMC) on PPARγ (Peroxisome Proliferator Activated Receptor gamma) and GLUT-4 (Glucose transporter-4) with respect to glucose transport. Various concentrations of MFMC ranging from 62.5 to 500 μg·mL-1 were evaluated for glucose uptake activity in vitro using L6 myotubes, rosiglitazone was used as a reference standard. The MFMC showed significant and dose-dependent increase in glucose uptake at the tested concentrations, further, the glucose uptake activity of MFMC (500 μg·mL-1) was comparable with rosigilitazone. Furthermore, MFMC has shown up-regulation of GLUT-4 and PPARγ gene expressions in L6 myotubes. In addition, the MFMC when incubated along with cycloheximide (CHX), which is a protein synthesis inhibitor, has shown complete blockade of glucose uptake. This indicates that new protein synthesis is required for increased GLUT-4 translocation. In conclusion, these findings suggest that MFMC is enhancing the glucose uptake significantly and dose dependently through the enhanced expression of PPARγ and GLUT-4 in vitro. © 2014 China Pharmaceutical University.


PubMed | Padmashree Institute of Management and Sciences, Bangalore University, Connexios Life science Pvt. Ltd. and Radiant Research Services Pvt. Ltd.
Type: Journal Article | Journal: Journal of intercultural ethnopharmacology | Year: 2016

The present study was undertaken to evaluate the effect of methanolic leaf extract of Gymnema sylvestre (MLGS) on glucose transport (GLUT) and insulin resistance in vitro.Peroxisome proliferator-activated receptor-gamma (PPAR-) and GLUT-4 expression were assessed in L6 myotubes for concluding the GLUT activity, and adiponectin and leptin expression was studied in 3T3 L1 murine adipocyte cell line to determine the effect of MLGS (250-750 g/ml) on insulin resistance.The findings of the experiments have demonstrated a significant and dose-dependent increase in glucose uptake in all the tested concentrations of MLGS, further the glucose uptake activity of MLGS (750 g/ml) was at par with rosiglitazone (50 g/ml). Concomitantly, MLGS has shown enhanced GLUT-4 and PPAR- gene expressions in L6 myotubes. Furthermore, cycloheximide (CHX) had completely abolished the glucose uptake activity of MLGS when co-incubated, which further confirmed that glucose uptake activity of MLGS was linked to enhanced expression of GLUT-4 and PPAR-. In addition, in another experimental set, MLGS showed enhanced expression of adiponectin and leptin, thus confirms the ameliorative effect of MLGS on insulin resistance.These findings suggest that MLGS has an enhanced glucose uptake activity in L6 myotubes, and ameliorate the insulin resistance in 3T3 L1 murine adipocyte cell line in vitro.


Kumaraswamy M.,Padmashree Institute of Management and Sciences | Anuradha M.,Padmashree Institute of Management and Sciences | Anuradha M.,Rishi Foundation and 234
Plant Tissue Culture and Biotechnology | Year: 2010

Multiple shoots from nodal explants of Pogostemon cablin Benth. MS supplemented with 0.5 mg/l BAP initiated a mean of 45.66 shoots/nodal explant. Within four weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm. Subsequent transfer of these in vitro derived nodal segments onto MS supplemented with BAP and Kn ( 0.5 mg/l), induced a mean of 62.45 shoots. Higher concentration of either BAP or Kn more than 0.5 mg/l resulted in callus proliferation and showed hyperhydric shoots with morphological abnormalities. Rooting was readily achieved upon transfer of shoots on half strength MS supplemented with 100 mg/l activated charcoal. Rooted shoots, following acclimatization in greenhouse, were successfully transferred to soil with 91% survival. Also shoots regenerated in vitro were directly transplanted to soil and acclimatized. Tissue cultured plants were analyzed for oil content by employing gas chromatography and found that the patterns were similar to mother plants. This confirms the true to type nature of micropropagated plants.


Kumara Swamy M.,Padmashree Institute of Management and Sciences | Balasubramanya S.,Padmashree Institute of Management and Sciences | Anuradha M.,Rishi Foundation and 234
African Journal of Biotechnology | Year: 2010

An efficient method was developed to initiate multiple shoots from nodal explants of Pogostemon cablin Benth. MS medium supplemented with 0.5 mg/L BA initiated a mean of 45.66 shoots/nodal explant. Within 4 weeks of initiation, regenerated multiple shoots attained a height of 3.6 cm. Subsequent transfer of these in vitro derived nodal segments onto MS medium supplemented with BA and KN (0.5 mg/L), induced mean of 62.45 shoots. Higher concentration of either BA or KN more than 0.5 mg/L resulted in callus proliferation and showed hyperhydric shoots with morphological abnormalities. Rooting was readily achieved upon transfer of shoots on half strength MS medium supplemented with 100mg/L activated charcoal. Rooted shoots, following acclimatization in green house, were successfully transferred to soil with 91% survival. Also shoots regenerated in vitro were directly transplanted to soil and acclimatized. Tissue cultured plants were analyzed for oil content by employing gas chromatography and found that the patterns were similar to mother plants. © 2010 Academic Journals.


Lokesh P.,Padmashree Institute of Management and Sciences | Balasubramanya S.,Rishi foundation and 234 | Anuradha M.,Rishi foundation and 234
Oriental Pharmacy and Experimental Medicine | Year: 2014

High-performance liquid chromatography is the prevalent method used to estimate Camptothecin quantitatively. In the present study thin layer chromatography and UV spectrophotometric method was established as a sensitive, economical and accurate method of detection and estimation of Camptothecin. The method was developed on TLC aluminum plates (60F254 from E-Merck Ltd.) precoated with silica gel using solvent system ethyl acetate: methanol (7.5:2.5, v/v) which gives a dark blue spot of Camptothecin at 254 nm (Rf value 0.46 cm). Spectrometric analysis of Camptothecin was carried out at the absorbance of 254 nm. Leaves and stems of Nothapodytes foetida and Ophiorrhiza mungos were extracted, subjected to TLC and UV spectrophotometric analysis and maximum absorption of CPT in ethyl acetate was observed at 254 nm. The quantification by this method was comparable to data obtained by HPLC. The optimized method was simple, repeatable, precise and cost effective, useful when there is large number of crude samples. In the present study this method was used to screen high yielding lines of CPT producing plants. © 2013 Institute of Korean Medicine, Kyung Hee University.


PubMed | Padmashree Institute of Management and Sciences, Bodoland University, Maharani Lakshmi Ammanni College for Women and Kalimpong College
Type: Journal Article | Journal: Antioxidants (Basel, Switzerland) | Year: 2016

Despite several pharmacological applications of Baccaurea ramiflora Lour., studies on the influence of its polyphenol content on pharmacological activity such as anti-inflammatory properties have been scarce. Here we evaluated in vitro antioxidant activity, poyphenolics by HPLC and the anti-inflammatory potential of the methanolic leaf extract of Baccaurea ramiflora (BME) and its protective effects in carrageenan-induced paw edema model of inflammation in rats. The BME extract contained 79.06 0.03 mg gallic acid equivalent (GAE)/g total polyphenols, 28.80 0.01 mg quercetin equivalent (QE)/g flavonoid and 29.42 0.01 g cathechin equivalent/g proanthocyanidin respectively and rosmarinic acid (8 mg/kg) as a main component was identified by HPLC. Results demonstrate that administration of BME at the dose of 200 mg/kg can reduce paw edema by over 63%, and it exhibits a dose-response effect. Depending on concentration, the extract exerted scavenging activity on DPPH radical (IC50 36.4 g/mL), significantly inhibited IL-1 (4.4 pg/mg protein) and TNF- (0.21 ng/g protein). Therefore, we conclude BME causes a substantial reduction of inflammation in in vivo models. We propose that rosmarinic acid and similar phenolic compounds may be useful in the therapy of inflammation-related injuries.


PubMed | Padmashree Institute of Management and Sciences, Maharani Lakshmi Ammanni College for Women and Rishi Foundation
Type: Journal Article | Journal: Iranian journal of pharmaceutical research : IJPR | Year: 2015

Leptadenia reticulata was reported to be used for several medicinal purposes. The present study was undertaken to evaluate anti-inflammatory, analgesic and lipid peroxidation inhibition activities of L. reticulata. The anti-inflammatory assay was performed by -carrageenan and formalin induced paw edema test. Pro inflammatory mediators (IL2, IL6, TNF-) in serum of treated and control organism were analyzed by quantitative ELISA. Lipid peroxidation inhibition was measured by thiobarbituric acid reactive substances (TBARS) assay. Analysis of the most active fraction revealed the presence of one phenolic compound (p-coumaric acid), two flavonoids (rutin and quercetin) which also determined quantitatively. The ethyl acetate fraction at 600 mg/Kg significantly inhibited -carrageenan and formalin induced paw edema by 60.59% and 59.24% respectively. Notable reduction in percentage of writhing (76.25%), induced by acetic acid signifies the potent analgesic activity. Lower level of pro-inflammatory cytokines (IL-2, IL-6, TNF-) in serum at the 4(th) hour of -Carrageenan injection indicated the inhibition of cyclooxigenase-2 (Cox-2), Nitric oxide (NO) and release of prostaglandin to prevent inflammation. The study also demonstrated the decrease in malonaldehyde (MDA) concentration which revealed the lipid peroxidation inhibition potential of the plant. Our finding provides evidence for potent biological activities in tested model which is supported by its characterized bioactive compounds and ethnomedicinal relevance.


Mohanty S.K.,Acharya Nagarjuna University | Malappa K.,Padmashree Institute of Management and Sciences | Godavarthi A.,Radiant Research Pvt. Ltd No 2 36 | Subbanarasiman B.,Rishi Foundation and 2365 19th Cross | And 3 more authors.
Asian Pacific Journal of Tropical Medicine | Year: 2014

Objective: To evaluate the antioxidant and anti proliferative potential of different solvent extract of micropropagated and naturally grown plants of Leptadenia reticulata against various cancer cell lines. Methods: In this study different extract were tested for cytotoxicity against human breast adenocarcinoma cell line MCF-7, human colon adenocarcinoma grade II cell line HT-29 and non cancer skeletal muscle cell line L6 through 3-(4, 5-dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide assay. The total antioxidant potential was estimated by three different antioxidant model diphenylpicrylhydrazyl free radical scavenging activity, H2O2 scavenging activity and FeCl3 reducing activity. Results: The ethyl acetate extract of both naturally grown plant and tissue cultured plant exhibited significant cytotoxicity with IC50 values of 21 μg/mL, 26 μg/mL and 22 μg/mL; 20 μg/mL, 30 μg/mL and 18 μg/mL respectively against three cell lines. The diphenylpicrylhydrazyl free radical scavenging activity was found to be highest with IC50 value of 267.13 μg/mL in ethyl acetate extract. The methanolic extract exhibited moderate antioxidant activity with IC50 value of 510.15 μg/mL. A highly positive correlation was observed between the antioxidant potential and cytotoxic activity of the plant. Conclusions: The strong cytotoxicity of ethyl acetate extract revealed anti carcinogenic potential of the plant which supports its traditional use as medicine. The present investigation is new to literature till date and will provide better scientific basis for future pharmacological, in vivo studies and novel source of pure bioactive compounds having anti cancer properties in this plant. © 2014 Hainan Medical College.


PubMed | Padmashree Institute of Management and Sciences, Rishi Foundation and 2365, Acharya Nagarjuna University and Radiant Research Pvt. Ltd No 2 36
Type: | Journal: Asian Pacific journal of tropical medicine | Year: 2014

To evaluate the antioxidant and anti proliferative potential of different solvent extract of micropropagated and naturally grown plants of Leptadenia reticulata against various cancer cell lines.In this study different extract were tested for cytotoxicity against human breast adenocarcinoma cell line MCF-7, human colon adenocarcinoma grade II cell line HT-29 and non cancer skeletal muscle cell line L6 through 3-(4, 5-dimethyl thiazol-2-yl)-5-diphenyl tetrazolium bromide assay. The total antioxidant potential was estimated by three different antioxidant model diphenylpicrylhydrazyl free radical scavenging activity, H2O2 scavenging activity and FeCl3 reducing activity.The ethyl acetate extract of both naturally grown plant and tissue cultured plant exhibited significant cytotoxicity with IC50 values of 21 g/mL, 26 g/mL and 22 g/mL; 20 g/mL, 30 g/mL and 18 g/mL respectively against three cell lines. The diphenylpicrylhydrazyl free radical scavenging activity was found to be highest with IC50 value of 267.13 g/mL in ethyl acetate extract. The methanolic extract exhibited moderate antioxidant activity with IC50 value of 510.15 g/mL. A highly positive correlation was observed between the antioxidant potential and cytotoxic activity of the plant.The strong cytotoxicity of ethyl acetate extract revealed anti carcinogenic potential of the plant which supports its traditional use as medicine. The present investigation is new to literature till date and will provide better scientific basis for future pharmacological, in vivo studies and novel source of pure bioactive compounds having anti cancer properties in this plant.


Sibi G.,Padmashree Institute of Management and Sciences | Devi A.P.,Padmashree Institute of Management and Sciences | Fouzia K.,Padmashree Institute of Management and Sciences | Patil B.R.,Padmashree Institute of Management and Sciences
Research Journal of Microbiology | Year: 2011

Urinary Tract Infection (UTI) defines a condition in which the urinary tract is infected with a pathogen causing inflammation which is a common, distressing and occasionally life threatening condition. UTI in patients with diabetes mellitus are reported with asymptomatic bacteriuria. To ensure appropriate therapy, current knowledge of the organisms that cause UTI and their antibiotic susceptibility is mandatory. This study focused on the frequency of uropathogens and their antibiotic susceptibility in different gender of diabetic patients. Biochemical characterization of uropathogens revealed the prevalence of gram negative organisms and E. coli as the predominant isolate. Among the antibiotics tested, trimethoprim was found to be effective for empirical treatment of UTI and has covered the majority of urinary pathogens followed by nalidixic acid, chloramphenicol and kanamycin. Most of the isolates were resistant to oxytetracycline which is more frequently prescribed and indicates that increased consumption of a particular antibiotic can be a pathway to its resistance by the uropathogens. Conclusively, resistance rates among common uropathogens continue to evolve and appear to be increasing too many commonly used antimicrobial agents and a continued surveillance of resistance rates among uropathogens is needed to ensure appropriate recommendations for the treatment of the urinary tract infections. © 2011 Academic Journals Inc.

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