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Acharya A.,Dr Dy Patil Biotechnology And Bioinformatics Institute | Vaniawala S.,SN Genelab | Parekh H.,SASTRA University | Nagee A.,Ashok And Rita Patel Institute Of Integrated Study And Res In Biotech And Allied Science | And 3 more authors.
Current HIV Research | Year: 2013

A portion of the gag gene cDNA for p24 protein from 30 Indian HIV-1 proviral DNA was amplified by PCR and sequenced. Phylogenetic analysis with reference samples of A1, A2, B, C, D, F1, F2, G, H, J, K, N and O subtypes revealed that 29 test samples aligned with subtype C reference strain while 1 matched with HIV-1 subtype A. Multiple alignment of predicted amino acid sequence of the Indian test samples and reference C subtype of HIV-1 samples from other countries indicated a molecular signature by way of rigid conservation of the amino acid 'S' at position 41 of the gag p24 protein in all Indian HIV-1 samples analyzed in this study as opposed to 'T'in the same position in C subtype sequences from other parts of the world. A phylogenetic analysis and visualization of the resulting tree in radial position showed distinct clubbing of all Indian C subtypes and formation of a cluster when compared to C subtype sequences from other countries with a single Chinese sample as an exception which was found in the Indian cluster. The use of a portion of p24 gene sequence as tool for subtyping as well as phylogenetic grouping with special reference to its geographical location is discussed. © 2013 Bentham Science Publishers. Source

Dharmadhikari V.,Padmashree Dr Dy Patil Medical College And Research Center | Joseph T.,Padmashree Dr Dy Patil Medical College And Research Center | Kulkarni A.,Padmashree Dr Dy Patil Medical College And Research Center
International Journal of Pharma and Bio Sciences | Year: 2013

The aim of our study was to determine the bacteriological and clinical profile of community acquired pneumonia patients requiring hospital admission. CAP was defined as per BTS guidelines. 65/104 cases of study group turned out to be culture positive for definitive bacterial etiology. The Commonest cause for CAP was Streptococcus pneumoniae (19/65) followed by, Klebsiella pneumoniae (17/65), Staphylococcus aureus (13/65), Pseudomonas aeruginosa (8/65), Escherichia coli (4/65), Acinetobactor spp. (3/65). Smoking (52%) and chronic alcoholism (28%) were major risk factors and COPD (23%) and Diabetes mellitus (19%) were major co-morbidities associated with CAP in the study group. The mortality was 8% cases after therapy and Pseudomonas aeruginosa was commonest cause of it. Death occurred exclusively in elderly people, all of whom were suffering from co- morbidities and had an initial CURB-65 a score of three. Limitation of our study was the inability to isolate atypical micro organisms. This emphasizes the need for further studies. Source

Gupta N.,Padmashree Dr Dy Patil Medical College And Research Center | Gandham N.,Padmashree Dr Dy Patil Medical College And Research Center | Jadhav S.,Padmashree Dr Dy Patil Medical College And Research Center | Mishra R.N.,Padmashree Dr Dy Patil Medical College And Research Center
Journal of Natural Science, Biology and Medicine | Year: 2015

Background: Acinetobacter is clinically important pathogen with widespread resistance to various antibiotics. We assessed the incidence of Acinetobacter infection at a tertiary care hospital, analyze their resistance pattern and identify the production of extended spectrum β-lactamases (ESBLs) and metallo β-lactamases (MBLs). Materials and Methods: The study was conducted in tertiary care hospital, India over a period of 2 years. Acinetobacter species were isolated from various clinical samples received in Department of Microbiology. After identification, Acinetobacter isolates were speciated and antibiotic susceptibility was determined by the standard disc diffusion method. ESBL and MBL production was detected by the double disc synergy test and combined disc diffusion test respectively. Results: Of 3298 infected samples, 111 (3.36%) were found to be Acinetobacter. The most predominant species was Acinetobacter calcoaceticus-A. baumannii (Acb) complex (72%). High incidence of resistance was recorded for piperacillin (55%), followed by ceftriaxone (46%) and ceftazidime (46%). Isolation rate and antibiotic resistance was higher in the Intensive Care Units (ICUs) of the hospital. ESBL and MBL production was detected in 31.5% and 14.4% of the isolates respectively. Discussion and Conclusion: A high level of antibiotic resistance was observed in our study and maximum isolation rate of Acinetobacter was in the ICUs. Acb complex was the most predominant and most resistant species. The analysis of susceptibility pattern will be useful in understanding the epidemiology of this organism in our hospital setup, which will help in treating individual cases and controlling the spread of resistant isolates to other individuals. Source

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