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Angwin, CA, United States

Pacific Union College is a private liberal arts college located in Napa Valley, California. The campus is located in the upper valley town of Angwin, eight miles north of St. Helena, California and within the Howell Mountain wine appellation. It is the only four-year college in Napa County. A coeducational residential college, it serves an almost exclusively undergraduate student body, the overwhelming majority of which live on campus.PUC maintains full regional accreditation from the Western Association of Schools and Colleges and various programmatic accreditations. It is the only liberal arts college affiliated with the Adventist Church. It was the 12th college or university founded in the state of California. In the fall of 2011, enrollment at Pacific Union College stood at over 1,500 students. Students study a variety of courses offered by the school's 20 academic departments. The school offers over 70 undergraduate majors and one master's program. The campus occupies 150 acres of the college's 1,900 acres in property. Wikipedia.

Wu S.-M.,Chang Gung University | Cheng W.-L.,Chang Gung University | Lin C.D.,Pacific Union College | Lin K.-H.,Chang Gung University
Cellular and Molecular Life Sciences

The thyroid hormone 3,3′,5-triiodo-l-thyronine (T3) mediates several physiological processes, including embryonic development, cellular differentiation, metabolism, and the regulation of cell proliferation. Thyroid hormone receptors (TRs) generally act as heterodimers with the retinoid X receptor (RXR) to regulate target genes. In addition to their developmental and metabolic functions, TRs have been shown to play a tumor suppressor role, suggesting that their aberrant expression can lead to tumor transformation. Conversely, recent reports have shown an association between overexpression of wild-type TRs and tumor metastasis. Signaling crosstalk between T3/TR and other pathways or specific TR coregulators appear to affect tumor development. Since TR actions are complex as well as cell context-, tissue- and time-specific, aberrant expression of the various TR isoforms has different effects during diverse tumorigenesis. Therefore, elucidation of the T 3/TR signaling mechanisms in cancers should facilitate the identification of novel therapeutic targets. This review provides a summary of recent studies focusing on the role of TRs in hepatocellular carcinomas (HCCs). © 2012 Springer Basel AG. Source

Wilson R.L.,Urbana University | Wilson R.L.,Pacific Union College | Frisz J.F.,Urbana University | Frisz J.F.,Thermo Fisher Scientific | And 4 more authors.
Biophysical Journal

The clusters of the influenza envelope protein, hemagglutinin, within the plasma membrane are hypothesized to be enriched with cholesterol and sphingolipids. Here, we directly tested this hypothesis by using high-resolution secondary ion mass spectrometry to image the distributions of antibody-labeled hemagglutinin and isotope-labeled cholesterol and sphingolipids in the plasma membranes of fibroblast cells that stably express hemagglutinin. We found that the hemagglutinin clusters were neither enriched with cholesterol nor colocalized with sphingolipid domains. Thus, hemagglutinin clustering and localization in the plasma membrane is not controlled by cohesive interactions between hemagglutinin and liquid-ordered domains enriched with cholesterol and sphingolipids, or from specific binding interactions between hemagglutinin, cholesterol, and/or the majority of sphingolipid species in the plasma membrane. © 2015 Biophysical Society. Source

Though spirituality is often considered a private matter, this study employs the construct of spiritual labor to examine how spirituality might be managed, manipulated, and/or manufactured in organizations. Modeled on the construct of emotional labor, spiritual labor is defined as the commodification, codification, and regulation of organizational members' spirituality. Thematic analysis of documents and interviews with parochial boarding school faculty/staff illustrate how the codification of spirituality occurred both overtly in official documents and more subtly in undocumented institutional norms. The regulation of spiritual expectations included normative pressure from both colleagues and administrators. Spiritual labor often led to dissonance and a sense of hypocrisy. Members who highly identified with the organization experienced less dissonance. Implications for issues such as job satisfaction, socialization, and organizational identity are discussed. © The Author(s) 2010. Source

Lin Y.-H.,Chang Gung University | Liao C.-J.,Chang Gung University | Huang Y.-H.,Liver Research Center | Wu M.-H.,Chang Gung University | And 10 more authors.

MicroRNAs (miRNAs) are thought to control tumor metastasis through direct interactions with target genes. Thyroid hormone (T 3) and its receptor (TR) are involved in cell growth and cancer progression. However, the issue of whether miRNAs participate in T 3 /TR-mediated tumor migration is yet to be established. In the current study, we demonstrated that T 3 /TR negatively regulates mature miR-17 transcript expression, both in vitro and in vivo. Luciferase reporter and chromatin immunoprecipitation (ChIP) assays localized the regions responding to TR-mediated repression to positions -2234/-2000 of the miR-17 promoter sequence. Overexpression of miR-17 markedly inhibited cell migration and invasion in vitro and in vivo, mediated via suppression of matrix metalloproteinases (MMP)-3. Moreover, p-AKT expression was increased in miR-17-knockdown cells that led to enhanced cell invasion, which was blocked by LY294002. Notably, low miR-17 expression was evident in highly metastatic cells. The cell migration ability was increased by T 3, but partially reduced upon miR-17 overexpression. Notably, TRα1 was frequently upregulated in hepatocellular carcinoma (HCC) samples and associated with low overall survival (P=0.023). miR-17 expression was significantly negatively associated with TRα1 (P=0.033) and MMP3 (P=0.043) in HCC specimens. Data from our study suggest that T 3 /TR, miR-17, p-AKT and MMP3 activities are interlinked in the regulation of cancer cell metastasis. © 2013 Macmillan Publishers Limited. Source

Huang Y.-H.,Liver Research Center | Lin Y.-H.,Chang Gung University | Chi H.-C.,Chang Gung University | Liao C.-H.,Chang Gung University | And 10 more authors.
Cancer Research

Thyroid hormone (T3) signaling through the thyroid hormone receptor (TRα1) regulates hepatoma cell growth and pathophysiology, but the underlying mechanisms are unclear at present. Here, we have shown that the oncomir microRNA-21 (miR-21) is activated by T3 through a native T3 response element in the primary miR-21 promoter. Overexpression of miR-21 promoted hepatoma cell migration and invasion, similar to that observed with T3 stimulation in hepatoma cells. In addition, anti-miR-21-induced suppression of cell migration was rescued by T3. The Rac-controlled regulator of invasion and metastasis, T-cell lymphoma invasion and metastasis 1 (TIAM1), was identified as a miR-21 target additionally downregulated by T3. Attenuation and overexpression of miR-21 induced upregulation and downregulation of TIAM1, respectively. TIAM1 attenuation, in turn, enhanced migration and invasion via the upregulation of β-catenin, vimentin, and matrix metalloproteinase-2 in hepatoma cells. Notably, correlations between TRa1, miR-21, and TIAM1 expression patterns in animal models paralleled those observed in vitro. In the clinic, we observed a positive correlation (P=0.005) between the tumor/nontumor ratios of TRa1 and miR-21 expression, whereas a negative correlation (P = 0.019) was seen between miR-21 and TIAM1 expression in patients with hepatoma. Our findings collectively indicate that miR-21 stimulation by T3 and subsequent TIAM1 suppression promotes hepatoma cell migration and invasion. Cancer Res; 73(8); 2505-17. © 2013 American Association for Cancer Research. Source

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