Ozone Research Center

Havana, Cuba

Ozone Research Center

Havana, Cuba
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Fernandez L.A.,Ozone Research Center | Hernandez C.,Ozone Research Center | Bataller M.,Ozone Research Center | Veliz E.,Ozone Research Center | And 2 more authors.
Water and Environment Journal | Year: 2010

Cyclophosphamide is a hazardous cytostatic drug, which should be removed from wastewaters before disposals. Ozonation slowly inactivates this substance, requiring long reaction times to obtain degradation. In this paper, different advanced oxidation processes (AOPs) are studied for cyclophosphamide oxidative degradation in aqueous medium: ozone at several basic pH values, ozone/hydrogen peroxide at neutral and basic pHs with the addition of the peroxide at the beginning of ozonation. The influence of ozone concentration in the gas phase and gas flow is also discussed. Results show that, under the experimental conditions studied, the radical mechanism acting in the AOPs rapidly degrades cyclophosphamide. Additionally, second-order rate constants k d and k OH were determined for direct (molecular) and indirect (radical) reactions. Finally, reactions were carried out with the aim of isolating and identifying some of the degradation products. From ozonation at pH 9, 4-ketocyclophosphamide was identified as the main reaction product. © 2009 The Authors. Journal compilation © 2009 CIWEM.

Hidalgo-Tallon J.,University of Granada | Menendez-Cepero S.,Ozone Research Center | Vilchez J.S.,University of Granada | Rodriguez-Lopez C.M.,University of Granada | And 2 more authors.
Journal of Alternative and Complementary Medicine | Year: 2013

Objectives: The objectives of this study were to evaluate the effectiveness and tolerability of ozone therapy by rectal insufflation as add-on therapy in fibromyalgia management. Design: Patients with fibromyalgia received 24 sessions of ozone therapy during a 12-week period. At each session, the administered dose of ozone was 8 mg (200 mL of gas, at a concentration of 40 μg/mL). Ozone sessions were given 5 days a week during the first 2 weeks, twice a week from weeks 3-6, and weekly from weeks 7-12. Fibromyalgia Impact Questionnaire (FIQ) was the main outcome measure, and was administered at baseline and at weeks 4, 8, and 12. Secondary outcome measures, administered at baseline and at endpoint, were the Pittsburgh Sleep Quality Index, the Beck Depression Inventory, the State and Trait Anxiety Inventory, and the SF-12, the abbreviated form of the Short Form Health Survey. Emergent adverse reactions to treatment were recorded. Results: FIQ total scores decreased significantly during the study period, with the decrease being observed in the first 4 weeks of the study. Significant improvement was also seen both in depression scores and in the Physical Summary Score of the SF-12. Transient meteorism after ozone therapy sessions was the most frequently reported side-effect. Conclusions: At the dose and number of sessions used in this study, ozone therapy by rectal insufflation seems to be beneficial for physical symptoms and depression of fibromyalgia. © Copyright 2013, Mary Ann Liebert, Inc. 2013.

Rodriguez Z.Z.,Ozone Research Center | Guanche D.,Ozone Research Center | Alvarez R.G.,Ozone Research Center | Martinez Y.,Ozone Research Center | And 2 more authors.
Toxicology Mechanisms and Methods | Year: 2011

In endotoxic shock, variations are known to occur in different biochemical parameters of oxidative stress. Ozone oxidative preconditioning (OOP) is a good candidate to restore the redox balance on different tissue. This investigation examined the effect of OOP on different biomarkers of oxidative stress in hepatic tissue of mice treated with lipopolysaccharide (LPS). LPS doses of 30amg/kg were administered intraperitoneally (i.p.) and pretreatment with ozone/oxygen mixture (OOM) was applied i.p. at 0.2, 0.4, and 1.2?mg/kg once daily during 5 days before LPS injection. The mice were euthanized under ether atmosphere at different times, 1 and 24?h after LPS injection. Hepatic tissue from all animals was taken for biochemical determinations of oxidative stress parameters such as thiobarbituric acid reactive substances (TBARS) content and activity of antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPx), and glutathione S-transferase (GST). The results demonstrated that OOP reduces levels of TBARS content and increases the activity of GPx in hepatic tissue. In conclusion, OOP was able to recover the redox balance and in this way to protect the animals against the oxidative damage induced by endotoxemia. © 2011 Informa Healthcare USA, Inc.

Fernandez O.S.L.,University of Habana | Pantoja M.,University of Habana | Soto M.T.D.,University of Habana | Dranguet J.,University of Habana | And 4 more authors.
Neurological Research | Year: 2012

Introduction/objectives: Although inflammation in disc hernia (DH) has been recognized and it is a wellknown process mediated by loss of the cellular redox balance, only a few studies about the impact of chronic oxidative stress on this neurological disorder have been made. Ozone therapy has been widely used with clinical efficacy in DH. This work aimed at characterizing the systemic redox status of patients with low back pain and neck pain as well as studying if ozone oxidative post-conditioning modified the pathological oxidative stress and protected against oxidative protein damage and if there is any relationship between oxidative changes and pain in both DH. Methods: Redox status of 33 patients with diagnosis of DH by computerized axial tomography, nuclear magnetic resonance, and clinical evaluations was studied. Ozone was administered by paravertebral way. After ozone treatment, plasmatic levels of antioxidant/pro-oxidant markers, pain, and life quality disability parameters were evaluated. Results: One hundred percent of patients showed a severe oxidative stress. Major changes in superoxide dismutase activity, total hydroperoxides, advanced oxidation protein products, fructolysine content, and malondialdehyde were observed. After ozone oxidative post-conditioning, there was a re-establishment of patients' cellular redox balance as well as a decrease in pain in both DH. A relationship between indicators of oxidative protein damage and pain was demonstrated. Conclusions: Ozone therapy protected against oxidation of proteins and reduced the pain. Relationship between markers of oxidative protein damage, disability parameters, and pain suggests the role of oxidative stress in the pathological processes involved in DH. © W. S. Maney & Son Ltd 2012.

Menendez S.,Ozone Research Center | del Cerro A.,Ozone Research Center | Alvarez T.,Ozone Research Center | Hernandez F.,Pharmacy and Food Institute
Reviews on Recent Clinical Trials | Year: 2012

The aim of this study was to evaluate the efficacy of ozone therapy in the treatment of 50 patients with peripheral vestibulocochlear syndrome. Ozone was injected in the cervical region C2-C3, for 20 sessions. Evaluation criteria was based in the evolution of nystagmus, tinnitus, hearing loss and vertigo. Also, oxidative stress parameters were measured. Results demonstrated that patient improvements, according to vertigo, hearing loss, tinnitus and nystagmus, were of 90, 80, 65 and 100 %, respectively. These patients were initially under condition of systemic oxidative stress, however, at the end of the study a redox balance was achieved. No side effects were observed. © 2012 Bentham Science Publishers.

Menendez S.,Ozone Research Center | Falcon L.,Carlos J Finlay Hospital | Maqueira Y.,Carlos J Finlay Hospital
Mycoses | Year: 2011

The results of the use of ozonised sunflower oil (OLEOZON ®) in the treatment of onychomycosis, based on its known antimycotic action and good skin tolerance, by means of a controlled randomised phase III assay are presented. A total of 400 outpatients were randomly divided into two groups: experimental, treated with topical OLEOZON ®, two times per day and control, treated also two times per day, with ketoconazole cream 2%, for 3months. A patient was considered cured when the sick nails regained the normal colour, growth and thickness, with a negative mycological study. In the experimental group, a regression of signs was achieved from the first month of treatment, while in the control group, it was obtained after the third month of treatment. All patients treated with OLEOZON ® had improvement in their condition (9.5%) or were cured (90.5%). However, in the control group, only 13.5% of patients were cured, 27.5% improved and 59% remained the same, with significant differences between both the groups. After 1year of follow-up, experimental and control groups presented 2.8% and 44.4% of relapses, respectively. Topical OLEOZON ® demonstrated effectiveness in the treatment of onychomycosis, superior to that of ketoconazole. No side effects were observed. © 2010 Blackwell Verlag GmbH.

Venta M.B.,Ozone Research Center | Broche S.S.C.,University of Habana | Torres I.F.,Ozone Research Center | Perez M.G.,University of Habana | And 3 more authors.
Ozone: Science and Engineering | Year: 2010

Ozone is an effective alternative for the postharvest treatment of fruits and vegetables. The purpose of this study was to evaluate the ozone application in gaseous or aqueous phases for postharvest disinfection of tomatoes (Lycopersicon esculentumMill) cultivar FA-180. Fruits harvested at the breaker stage were exposed to ozone concentrations of 25 and 45 mg m-3 for 2 h per day during 16 days, at non-controlled temperature and relative humidity. Exposure to ozone during storage extends the shelf-life of tomatoes, besides preserving its sensory attributes. Mature tomatoes, inoculated with Escherichia coli ATCC 25922, were washed with ozonated water containing 0.5 to 1.0 mg L-1 during 15 to 30 min. To achieve an adequate disinfection, 1 mg L-1 and 15 min are recommended. The disinfection with ozonated water was effective. © 2010 International Ozone Association.

Copello M.,Retinitis Pigmentosa National Reference Center Dr Salvador Allende Hospital | Menendez S.,Ozone Research Center | Hernandez F.,University of Habana
Ozone: Science and Engineering | Year: 2012

Retinitis pigmentosa is characterized by bad night vision, decrease of visual field and/or acuity. The aim of this article is to assess ozone therapy's efficacy in these patients, treated twice a year over 20 years. Forty patients received ozone via the rectal way, during 20 sessions as the only treatment, with repetition of this cycle every 6 months, and 16 received other medical treatments (control group). A significant decrease of the visual field in the control group in respect to the ozone group was observed after 20 years of treatment. In the ozone group, 50% of patients improved their visual field, accompanied with homeostasis in redox state. It is recommended to apply ozone therapy at 6-month intervals in order to maintain visual capabilities.The opinions and conclusions expressed in this article are those of the authors and contributors, and do not necessarily reflect those of the International Ozone Association, the editors, Editorial Board, or Taylor & Francis. Readers are to make their own decisions with regard to the work presented. These medical articles are enclosed, as in the past, as a service to the members of the IOA interested in medical applications. © 2012 Copyright 2012 International Ozone Association.

Guanche D.,Ozone Research Center | Hernandez F.,Ozone Research Center | Zamora Z.,Ozone Research Center | Alonso Y.,Ozone Research Center
Toxicology Mechanisms and Methods | Year: 2010

The confirmed advantageous effects of oxygen/ozone therapy in several clinical conditions stimulated experimental studies on effects of the therapy in induced septic shock. This study researches the influence of Ozone Oxidative Pre-conditioning (OOP) in unbalance between pro-oxidant and antioxidant activity generated in liver and lung during a process of sepsis. The study was conducted on male rats. Sepsis was induced by intraperitoneal injection of fecal material and pre-treatment with ozone/oxygen mixture was administered before fecal material injection. Activities of catalase, glutathione peroxide, and superoxide dismutase were measured, as well as conjugated dienes (CD) and thiobarbituric acid reactive substances (TBARS) were estimated. The results demonstrated that OOP not cause oxidative damage. It reduced levels of pro-oxidant biomarkers in lung and liver, with decreased total pro-oxidant activity and elevated total antioxidant activity from a system for diagnosis of oxidative stress in both tissues. These results suggest that OOP protected liver and lung for oxidative stress in septic shock. © 2010 Informa Healthcare USA, Inc.

PubMed | Ozone Research Center
Type: Journal Article | Journal: Inflammation research : official journal of the European Histamine Research Society ... [et al.] | Year: 2012

Reactive oxygen and nitrogen species are involved in the pathogenesis of sepsis syndrome with peritonitis and the septic shock. The aim of this study was to determine whether ozone oxidative preconditioning (OOP) may exert beneficial effects in the prevention and treatment of sepsis syndrome in rats inoculated by the intraperitoneal route (i.p.) with fecal material and also to determine if antioxidant enzymes such as superoxide dismutase (SOD) and glutathione peroxidase (GPx) may exert protective effects against this systemic inflammatory disorder.Male Wistar rats were used. SOD and GPx activities were determined in erythrocytes. Thiobarbituric acid reactive substances (TBARS) content as biomarkers of oxidative stress, alanine amino transferase (ALT), aspartate amino transferase (AST) and creatinine (CRE) were measured in blood serum and myeloperoxidase (MPO) in lung tissue as markers of organs damage.In rats submitted to OOP, SOD and GPx activities were significantly increased and it was accompanied by significant decrease of TBARS content in blood serum. OOP also significantly reduced levels of ALT, AST and CRE in blood serum as well as MPO in rat lung.The results support the important role of SOD and GPx in the protective effects of OOP against organ damage induced by fecal peritonitis in rats.

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