Marseille, France
Marseille, France

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Sapet C.,OZ Biosciences | Laurent N.,OZ Biosciences | de Chevigny A.,French National Center for Scientific Research | Le Gourrierec L.,OZ Biosciences | And 3 more authors.
BioTechniques | Year: 2017

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofectionbased method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation. © 2017, Eaton Publishing Company. All rights reserved.


Plank C.,TU Munich | Eglin D.,AO Research Institute Davos | Fahy N.,National University of Ireland | Sapet C.,OZ Biosciences | And 6 more authors.
European Journal of Nanomedicine | Year: 2012

The GAMBA Consortium is developing a novel gene-activated matrix platform for bone and cartilage repair with a focus on osteoarthritis-related tissue damage. The scientific and technological objectives of this project are complemented with an innovative program of public outreach, actively linking patients and society to the evolvement of this project. The GAMBA platform will implement a concept of spatiotemporal control of regenerative bioactivity on command and demand. A gene activated matrix is a biomaterial with embedded gene vectors that will genetically modify cells embedded in or colonising the matrix. The platform comprises modules that self-adapt to the biological environment and that can be independently addressed with endogenous biological and exogenous physical or pharmacological stimuli, resulting in a temporally and spatially coordinated growth factor gene expression pattern. This reproduces, within the matrix, key elements of natural tissue formation. The modules are a biomimetic hyaluronan gel, a ceramic matrix, growth factor-encoding gene vector nanoparticles, magnetic nanoparticles and mesenchymal stem cells. Anatomical adaptivity is achieved with engineered thermal properties of the polymer matrix, which embeds other modules, selected according to functional requirements. Mechanical support is provided by Micro Macroporous Biphasic Calcium Phosphate (MBCP ™ ), a resorbable material approved for clinical use. Spatiotemporal control of bioactivity and responsiveness to physiological conditions is represented, firstly, in the spatial distribution and release profiles of gene vectors within the composite matrix and, secondly, by letting local and external biological or physical stimuli activate the promoters driving the expression of vector-encoded growth factor transgenes. This concept is implemented by a multidisciplinary team from leading European institutions. Here, we report on the concepts, objectives and some preliminary results of the GAMBA project which is funded in 7th Framework Programme of the European Union THEME [NMP-2009-2.3-1], Biomimetic gels and polymers for tissue repair. © 2012 by Walter de Gruyter • Berlin • Boston.


Sapet C.,OZ Biosciences | Laurent N.,OZ Biosciences | de Chevigny A.,French National Center for Scientific Research | le Gourrierec L.,OZ Biosciences | And 3 more authors.
BioTechniques | Year: 2011

Primary neural stem cells (NSCs) can be cultivated and differentiated in vitro but are difficult to transfect using conventional methods. We describe a simple and rapid magnetofection-based method suitable for the lab bench as well as for high-throughput projects. Our method yields high transfection efficiency and can be used for deciphering the genetic control of neural cell differentiation.


Orlando C.,Institute for Experimental Treatment of Cystic Fibrosis | Orlando C.,University of Zürich | Castellani S.,University of Foggia | Mykhaylyk O.,TU Munich | And 6 more authors.
Journal of Gene Medicine | Year: 2010

Background: Lentiviral (LV) vectors are able to only slowly and inefficiently transduce nondividing cells such as those of the airway epithelium. To address this issue, we have exploited the magnetofection technique in in vitro models of airway epithelium. Methods: Magnetofectins were formed by noncovalent interaction between LV particles and polycation-coated iron oxide nanoparticles. Efficiency of LV-mediated transduction (as evaluated through green fluorescent protein (GFP) expression by cytofluorimetric analysis) was measured in bronchial epithelial cells in the presence or absence of a magnetic field. Cytotoxicity was evaluated by lactate dehydrogenase (LDH) release; cell monolayer integrity by measurement of transepithelial resistance (TER) and evaluation of correct zonula occludens-1 (ZO-1) localization at tight junctions (TJs) by immunofluorescence and confocal microscopy. Results: In nonpolarized cells, magnetofectins enhanced LV-mediated transduction at multiplicity of infection (MOI) of 50 up to 3.9-fold upon a 24-h incubation, to levels that approached those achieved at MOI of 200 for LV alone, in the presence or absence of the magnetic field. Magnetofection significantly increased the percentage of transduced cells up to 186-fold already after 15 min of incubation. In polarized cells, magnetofection increased GFP+ cells up to 24-fold compared to LV alone. Magnetofection did not enhance LDH release and slightly altered TER but not ZO-1 localization at the TJs. Conclusions: We conclude that magnetofection can facilitate in vitro LV-mediated transduction of airway epithelial cells, in the absence of overt cytotoxicity and maintaining epithelial integrity, by lowering the necessary vector dose and reducing the incubation time required to achieve efficient transduction. Copyright © 2010 John Wiley & Sons, Ltd.


Sapet C.,Oz Biosciences | Laurent N.,Oz Biosciences | Gourrierec L.L.,Oz Biosciences | Augier S.,Oz Biosciences | Zelphati O.,Oz Biosciences
Annales de Biologie Clinique | Year: 2010

Gene therapy offers exciting opportunities for the treatment of innate or acquired genetic diseases. However, there is still a need for a safe and efficient strategy to deliver nucleic acids into cells while overcoming the current limitations faced with standard viral vectors. Intensive researches have been carried out over the past decade, focusing both on viral and non-viral (i.e. physical or chemical) strategies. Of these numerous attempts, magnetofection, defined as the combination of nucleic acid vectors with magnetic nanoparticles, holds the promise to achieve high transfection efficiency with reduced toxicity by magnetically focusing the genetic material to be delivered on its cellular target. In vitro as well as in vivo results already demonstrated that this strategy may become a valuable tool towards practical gene therapy.


Sapet C.,OZ Biosciences | Pellegrino C.,French Institute of Health and Medical Research | Laurent N.,OZ Biosciences | Sicard F.,OZ Biosciences | Zelphati O.,OZ Biosciences
Pharmaceutical Research | Year: 2012

Purpose: Adenoviruses are among the most powerful gene delivery systems. Even if they present low potential for oncogenesis, there is still a need for minimizing widespread delivery to avoid deleterious reactions. In this study, we investigated Magnetofection efficiency to concentrate and guide vectors for an improved targeted delivery. Method: Magnetic nanoparticles formulations were complexed to a replication defective Adenovirus and were used to transduce cells both in vitro and in vivo. A new integrated magnetic procedure for cell sorting and genetic modification (i-MICST) was also investigated. Results: Magnetic nanoparticles enhanced viral transduction efficiency and protein expression in a dose-dependent manner. They accelerated the transduction kinetics and allowed non-permissive cells infection. Magnetofection greatly improved adenovirus-mediated DNA delivery in vivo and provided a magnetic targeting. The i-MICSTresults established the efficiency of magnetic nanoparticles assisted viral transduction within cell sorting columns. Conclusion: The results showed that the combination of Magnetofection and Adenoviruses represents a promising strategy for gene therapy. Recently, a new integrated method to combine clinically approved magnetic cell isolation devices and genetic modification was developed. In this study, we validated that magnetic cell separation and adenoviral transduction can be accomplished in one reliable integrated and safe system. © Springer Science+Business Media, LLC 2011.


PubMed | Oz Biosciences
Type: Journal Article | Journal: Annales de biologie clinique | Year: 2010

Gene therapy offers exciting opportunities for the treatment of innate or acquired genetic diseases. However, there is still a need for a safe and efficient strategy to deliver nucleic acids into cells while overcoming the current limitations faced with standard viral vectors. Intensive researches have been carried out over the past decade, focusing both on viral and non-viral (i.e. physical or chemical) strategies. Of these numerous attempts, magnetofection, defined as the combination of nucleic acid vectors with magnetic nanoparticles, holds the promise to achieve high transfection efficiency with reduced toxicity by magnetically focusing the genetic material to be delivered on its cellular target. In vitro as well as in vivo results already demonstrated that this strategy may become a valuable tool towards practical gene therapy.


A subject of the present invention is the development of a novel family of cationic lipids and their use as vectors for in vitro, ex vivo and in 5 vivo delivery of biologically active agents.


Trademark
Oz Biosciences | Date: 2015-10-14

Chemical products for use in industry, science, photography, as well as in agriculture, horticulture and forestry; chemical reagents other than for medical or veterinary use; biochemical, chemical and biological reagents for use in scientific research, in industry and science; chemical, biochemical and biological products for scientific, medical or pharmaceutical research. Pharmaceutical and veterinary products; sanitary products for medical purposes; dietetic food and substances for medical or veterinary use; food for babies; food supplements for humans and animals; materials for dressings; material for dental fillings and dental impressions; disinfectants; products for destroying vermin; fungicides, herbicides; bath preparations for medical use; sanitary panties or napkins; chemical preparations for medical or pharmaceutical use; medicinal herbs; herbal teas; parasiticides; alloys of precious metals for dental use; chemical, biochemical and biological products for medical or pharmaceutical use. Evaluations and assessments in the fields of science and technology provided by engineers; scientific and technical research; research and development of new products for others; technical project studies; scientific research for medical purposes; research and development of new products for applications in life sciences, in the pharmaceutical, parapharmaceutical and veterinary fields; research in biology, bacteriology, chemistry, cosmetology and physics.


PubMed | OZ Biosciences
Type: Journal Article | Journal: Therapeutic delivery | Year: 2012

In recent years, gene therapy has received considerable interest as a potential method for the treatment of numerous inherited and acquired diseases. However, successes have so far been hampered by several limitations, including safety issues of viral-based nucleic acid vectors and poor in vivo efficiency of nonviral vectors. Magnetofection has been introduced as a novel and powerful tool to deliver genetic material into cells. This technology is defined as the delivery of nucleic acids, either naked or packaged (as complexes with lipids or polymers, and viruses) using magnetic nanoparticles under the guidance of an external magnetic field. This article first discusses the principles of the Magnetofection technology and its benefits as compared with standard transfection methods. A number of relevant examples of its use, both in vitro and in vivo, will then be highlighted. Future trends in the development of new magnetic nanoparticle formulations will also be outlined.

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