Manchester, United Kingdom
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Patent
Oxyrane UK, Ghent University and Vib Vzw | Date: 2012-09-14

Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.


Patent
Oxyrane UK, Ghent University and Vib Vzw | Date: 2012-10-10

1. A method of producing an altered N-glycosylation form of a target protein or polypeptide, comprises:a) providing a cell genetically engineered to comprise at least one modified N-glycosylation activity and introducing into the cell a nucleic acid encoding the target protein or polypeptide, wherein the cell produces the target protein or polypeptide in an altered N-glycosylation form, the cell being a Yarrowia lipolytica cell, an Arxula adeninivorans or a cell from a related species of dimorphic yeast; orb) contacting the target protein or polypeptide with a cell lysate prepared from a cell genetically engineered to comprise at least one modified N-glycosylation activity, the cell being a Yarrowia lipolytica cell, an Arxula adeninivorans or a related species of dimorphic yeast;wherein the at least one modified N-glycosylation activity comprises a deficiency in OCH 1 activity and the target protein or polypeptide is useful in the pharmaceutical, food, beverage, agriculture or chemical industries.


Patent
Oxyrane UK, Ghent University and Vib Vzw | Date: 2012-09-14

Described herein are methods and genetically engineered cells useful for producing an altered N-glycosylation form of a target molecule. Also described are methods and molecules with altered N-glycosylation useful for treating a variety of disorders such as metabolic disorders.


Patent
Oxyrane UK, Ghent University and Vib Vzw | Date: 2012-10-10

The present invention provides means and methods for enhancing the production of membrane or secreted proteins in genetically engineered cells. In particular, the invention provides nucleic acid molecules encoding HAC1 proteins and uses thereof.


Patent
Oxyrane UK, Ghent University and Vib Vzw | Date: 2012-10-10

The present invention provides means and methods for enhancing the production of membrane or secreted proteins in genetically engineered cells. In particular, the invention provides nucleic acid molecules encoding HAC1 proteins and uses thereof.


The invention provides mannosidases capable of uncapping mannose-1-phospho-6-mannose moieties and demannosylating phosphorylated N-glycans, methods of using such mannosidases, glycoproteins produced using the methods, as well as methods of facilitating mammalian cellular uptake of glycoproteins.


Described herein are methods and materials for reducing degradation of recombinant proteins in fungal cells such as Yarrowia.


Patent
Oxyrane UK | Date: 2015-12-28

This document relates to molecular complexes having acid alpha glucosidase activity and at least one modification that results in enhanced ability of the molecular complex to be transported to the interior of a mammalian cell.


Patent
Oxyrane UK | Date: 2015-03-06

Described herein are methods and genetically engineered fungal cells useful for producing target molecules containing mammalian-like complex N-glycans or containing intermediates in a mammalian glycosyiation pathway.


Patent
Oxyrane UK | Date: 2013-03-14

This document relates to molecular complexes having acid alpha glucosidase activity and at least one modification that results in enhanced ability of the molecular complex to be transported to the interior of a mammalian cell.

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