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Song J.,Sungkyunkwan University | Kim M.-J.,Kangwon National University | Kim Y.-J.,OTTOGI Corporation | Lee J.,Sungkyunkwan University
Food Chemistry | Year: 2017

Oxidation products and tocol homologues were monitored in oils during chicken frying to determine the discarding point of highly used frying oils. Oils were heated without chicken for 170 h while chicken frying was performed 130 cycles at 180 °C. As heating time and frying cycles increased, all oxidation parameters including acid value, total polar materials (TPM), conjugated dienoic acid (CDA), and p-anisidine values (p-AV) increased significantly (p < 0.05). γ-Tocopherol and γ-tocotrienol had the lowest stability in oils during heating or frying processes compared to other tocol homologues. TPM values over 24% were obtained after about 109 h for heated oil and 100 cycles for oils used to fry chicken. A decrease of 2,2-diphenyl-1-picrylhydrazyl (DPPH) in isooctane and methanol was highly correlated with the formation of TPM in oils during the frying process. Both DPPH loss and TPM values could be applied to determine the discarding points of highly used frying oils. © 2016 Elsevier Ltd

PubMed | Amore Pacific, OTTOGI Corporation, Life Ingredient & Material Research Institute, University of Southern California and Seoul National University of Science and Technology
Type: | Journal: Enzyme and microbial technology | Year: 2016

Steviol is a diterpene isolated from the plant Stevia rebaudiana that has a potential role as an antihyperglycemic agent by stimulating insulin secretion from pancreatic beta cells and also has significant potential to diminish the renal clearance of anionic drugs and their metabolites. In this study, the lacS gene, which encodes a thermostable -glycosidase (SSbgly) enzyme from the extremely thermoacidophillic archaeon Sulfolobus solfataricus, was cloned and expressed in E. coli Rossetta BL21(DE3)pLyS using lactose as an inducer. Through fermentation, SSbgly was expressed as a 61kDa protein with activity of 24.3U/mg and the OD

Jung E.,Sookmyung Womens University | Kim Y.,OTTOGI Corporation | Joo N.,Sookmyung Womens University
Journal of the Science of Food and Agriculture | Year: 2013

BACKGROUND: The therapeutic action of a plant depends on its chemical constituents. In this study, experiments were carried out in order to evaluate the effect of extraction conditions on the antioxidative and antimicrobial activities of Roselle (Hibiscus sabdariffa L.). RESULTS: Roselle was found to be rich in malic acid, anthocyanins, ascorbic acid and minerals, especially Ca and Fe, but low in glucose. More than 18 volatile compounds were identified by gas chromatography and gas chromatography-mass spectrometry. This herb, which is rich in phenolic compounds and displays DPPH radical scavenging activity, could be a good source of natural antioxidants. The antimicrobial activity of the Roselle water and ethanol extracts was tested with Bacillus subtilis (ATCC6633), Staphylococcus aureus (ATCC6538) and Escherichia coli (ATCC 8739). The inhibition of the Roselle ethanol extract against B. subtilis and S. aureus was slightly higher than that of water extract but this difference was not significant. However, E. coli was strongly inhibited by the Roselle water extract at concentrations of 25 and 50 mg mL-1 as determined by a paper disc method. CONCLUSION: The obtained results indicated that antioxidant and antimicrobial activity was related to different methods of extraction and Roselle extracts could be a source of therapeutically useful products. © 2013 Society of Chemical Industry.

Song J.,Sungkyunkwan University | Jang E.Y.,Sungkyunkwan University | Kim M.-J.,Kangwon National University | Kim Y.-J.,Ottogi Corporation | Lee J.,Sungkyunkwan University
International Journal of Food Science and Technology | Year: 2016

A simple spectroscopic method using 2,2-diphenyl-1-picrylhydrazyl (DPPH) was developed to simultaneously monitor the contents of free radical scavenging antioxidants (FRSs) and oxidised lipid products during oxidation through selection of proper solvents. Validation of the DPPH method was conducted in a stripped oil matrix, and practical applicability of the method was tested with heated oils. The absorbance of DPPH in isooctane simultaneously reflected the changing amounts of both FRSs and oxidised lipid products, whereas the absorbance in methanol mainly reflected changes in FRSs. Total polar materials (TPMs) were found to be representative of oxidised lipid products, which reacted with DPPH in isooctane better than in methanol. Validation parameters including accuracy, precision, linearity, limit of detection and limit of quantification were determined using α-tocopherol as a model for FRSs and TPMs for oxidised lipid products. The DPPH protocol in isooctane and in methanol is useful for assessing the degree of oxidation in heated oils through comparing the contents of FRSs and oxidised lipids. © 2016 Institute of Food Science and Technology

PubMed | Ottogi Corporation, Chonbuk National University and Seoul National University
Type: Journal Article | Journal: BMC complementary and alternative medicine | Year: 2016

Curcumin, a major active component of turmeric, has previously been reported to alleviate liver damage. Here, we investigated the mechanism by which turmeric and curcumin protect the liver against carbon tetrachloride (CCl4)-induced injury in rats. We hypothesized that turmeric extract and curcumin protect the liver from CCl4-induced liver injury by reducing oxidative stress, inhibiting lipid peroxidation, and increasing glutathione peroxidase activation.Chronic hepatic stress was induced by a single intraperitoneal injection of CCl4 (0.1ml/kg body weight) into rats. Turmeric extracts and curcumin were administered once a day for 4weeks at three dose levels (100, 200, and 300mg/kg/day). We performed ALT and AST also measured of total lipid, triglyceride, cholesterol levels, and lipid peroxidation.We found that turmeric extract and curcumin significantly protect against liver injury by decreasing the activities of serum aspartate aminotransferase and alanine aminotransferase and by improving the hepatic glutathione content, leading to a reduced level of lipid peroxidase.Our data suggest that turmeric extract and curcumin protect the liver from chronic CCl4-induced injury in rats by suppressing hepatic oxidative stress. Therefore, turmeric extract and curcumin are potential therapeutic antioxidant agents for the treatment of hepatic disease.

Kang C.-H.,Jeju National University | Choi Y.H.,Korea University | Park S.-Y.,OTTOGI Ltd. | Kim G.-Y.,Jeju National University
Journal of Medicinal Food | Year: 2012

The methanol extract of Codium fragile (MECF) has been reported to possess bioactive properties such as antidegranulation in eosinophils, as well as anti-edema, antibacterial, and antiviral activities. However, little is known about the molecular effects of MECF on lipopolysaccharide (LPS)-induced inflammation. Therefore, we investigated whether MECF affects the expression of inflammatory mediators in LPS-stimulated RAW 264.7 cells. To evaluate the anti-inflammatory effects of MECF, the cells were pretreated with MECF for 1 hour and then cultured with LPS for 24 hours. Our results indicate that MECF significantly attenuated secretion of LPS-induced inflammatory mediators nitric oxide (NO), prostaglandin E 2 (PGE 2), and tumor necrosis factor (TNF)-α in RAW 264.7 cells. Additionally, LPS-induced mRNA and protein expression of inducible NO synthase (iNOS), cyclooxygenase (COX)-2, and TNF-α was decreased by pretreatment with MECF. These data indicate that MECF attenuates the expression of these inflammatory mediators at the transcriptional level. Therefore, we also investigated the effects of MECF on nuclear factor-κB (NF-κB) activity, which may be an important transcriptional factor for regulating the expression of iNOS, COX-2, and TNF-α mRNA. Our results showed that MECF reduced LPS-induced NF-κB activity via the suppression of nuclear translocation of the p50 and p65 NF-κB subunits and degradation of inhibitor of κB. In conclusion, we propose that MECF treatment down-regulates the expression and secretion of LPS-induced inflammatory mediators by inhibiting NF-κB activity. © Copyright 2012, Mary Ann Liebert, Inc.

Moon D.-O.,Jeju National University | Kim M.-O.,Jeju National University | Kang S.-H.,Jeju National University | Choi Y.H.,Korea University | And 2 more authors.
Cancer Letters | Year: 2010

Nuclear factor-kappa B (NF-κB) activation by tumor necrosis factor-alpha (TNF-α) attenuates the TNF-α-induced apoptosis pathway. Thus, blockage of NF-κB activity may improve the anti-cancer activity of TNF-α HA14-1 induces apoptosis in various human cancer cells, and the molecular mechanisms of this action remain to be fully characterized. The present study evaluated the involvement of NF-κB, reactive oxygen species (ROS), and c-Jun N-terminal kinase (JNK) in the effects of HA14-1 by examining the sensitization effect on TNF-α-induced apoptosis in human leukemia cells. Such sensitization is closely associated with the inhibitory effect of HA14-1 on TNF-α-mediated NF-κB activation. HA14-1 suppressed NF-κB activation through inhibition of phosphorylation and degradation of IκBα. This inhibition was correlated with suppression of NF-κB-dependent gene products (c-myc, cyclin D1, cox-2, and IAP-1). Additionally, the present findings provide evidence of a critical role of ROS accumulation induced by HA14-1 in TNF-α-induced apoptosis. Moreover, HA14-1 also markedly sustained TNF-α-mediated JNK activation. A specific JNK inhibitor abolished the sensitization effect of HA14-1 on TNF-α-induced apoptosis. Taken together, these results indicate that ROS and JNK represent important signals in HA14-1 sensitization in TNF-α-induced apoptosis. © 2009 Elsevier Ireland Ltd.

Moon D.-O.,Daegu University | Park S.-Y.,OTTOGI Ltd. | Choi Y.H.,Korea University | Ahn J.S.,Korea Research Institute of Bioscience and Biotechnology | Kim G.-Y.,Jeju National University
Biochemical Pharmacology | Year: 2011

Guggulsterone (GGS) has anti-tumor and anti-angiogenesis potential by suppressing nuclear factor-κB and STAT3 activity. Although GGS has been suggested as a potential therapeutic agent for treating various cancers, the underlying molecular mechanisms are unknown. Therefore, we investigated whether GGS sensitizes hepatocellular carcinoma cells (HCC) to apoptosis mediated by tumor necrosis factor-related apoptosis inducing ligand (TRAIL). The apoptotic mechanism induced by treatment with a GGS/TRAIL combination involved the loss of mitochondrial transmembrane potential and consequent activation of caspases. GGS also induced upregulation of the death receptor DR5 for TRAIL. The effects seemed to be associated with eIF2α and CHOP activation, which are related to the endoplasmic reticulum (ER) stress response and apoptosis. This relationship was suggested by the observation that CHOP downregulation by specific siRNA attenuated both GGS-mediated DR5 upregulation and the cytotoxicity induced by GGS/TRAIL co-treatment. Moreover, salubrinal, a specific eIF-2α phosphorylation-inducing agent, enhanced the expression of CHOP and DR5 induced by GGS and sensitized cells to GGS/TRAIL-induced apoptosis. Thus, GGS-induced eIF2α phosphorylation seems to be important for CHOP and DR5 upregulation. Furthermore, these events were accompanied by an increase in the generation of reactive oxygen species. Pretreatment with N-acetyl-l-cysteine and glutathione inhibited GGS-induced ER-stress, and CHOP and DR5 upregulation and almost completely blocked GGS/TRAIL-induced apoptosis. These results collectively indicate that DR5 induction via eIF-2α and CHOP is crucial for the marked synergistic effects induced by TRAIL and GGS. Taken together, these results indicate that a GGS/TRAIL combination could represent a novel important tool for cancer therapy. © 2011 Elsevier Inc.

PubMed | OTTOGI Corporation and Seoul National University of Science and Technology
Type: | Journal: Food chemistry | Year: 2016

Curcuminoids from rhizomes of Curcuma longa possess various biological activities. However, low aqueous solubility and consequent poor bioavailability of curcuminoids are major limitations to their use. In this study, curcuminoids extracted from turmeric powder using stevioside (Ste), rebaudioside A (RebA), or steviol glucosides (SG) were solubilized in water. The optimum extraction condition by Ste, RebA, or SG resulted in 11.3, 9.7, or 6.7mg/ml water soluble curcuminoids. Curcuminoids solubilized in water showed 80% stability at pH from 6.0 to 10.0 after 1week of storage at 25C. The particle sizes of curcuminoids prepared with Ste, RebA, and SG were 110.8, 95.7, and 32.7nm, respectively. The water soluble turmeric extracts prepared with Ste, RebA, and SG showed the 2,2-diphenyl-1-picrylhydrazyl radical scavenging (SC50) activities of 127.6, 105.4, and 109.8g/ml, and the inhibition activities (IC50) against NS2B-NS3(pro) from dengue virus type IV of 14.1, 24.0 and 15.3g/ml, respectively.

Ottogi Ltd. | Date: 2014-02-12

Asian noodles; Cookies; Maple syrup; Noodles.

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