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Chiraz B.S.,Tunis el Manar University | Myriam A.,Tunis el Manar University | Ines Z.,La Rabta Hospital | Catherine J.,University of Washington | And 10 more authors.
Molecular Biology Reports | Year: 2014

A deletion of two genes from the late cornified envelope (LCE), LCE3B and LCE3C within epidermal differentiation complex on chromosome 1 was shown to be associated with both psoriasis and psoriatic arthritis (PsA) in several populations. To assess whether this deletion may contribute to the genetic predisposition to PsA in Tunisia, a total of 73 patients with PsA and 120 healthy matched controls were screened for the deletion, LCE3C-LCE3B-del, and its tag SNP, rs4112788. We also evaluated a possible relationship between PSORS1 and LCE3C-LCE3B-del through genotyping two proxy markers to HLA-C (rs12191877 and rs2073048). Our results did not provide evidence for association between the LCE3C-LCE3B-del nor the rs4112788 and the PsA. Similarly, no significant epistatic effect was observed. Our data suggest that The LCE deletion, previously identified in patients with psoriasis, is not of a major importance in the development of PsA in Tunisian patients supporting the current perception that different genetic risk factors contribute to skin and joint disease. However, these results need to be confirmed by additional large-scale studies of Tunisian PsA patients and controls. © 2014 Springer Science+Business Media. Source

Sassi N.,Osteoarthritis Osteoporosis Research Laboratory | Laadhar L.,LaRabta Hospital | Driss M.,Salah Azaiez Health Institute | Kallel-Sellami M.,LaRabta Hospital | And 2 more authors.
Arthritis Research and Therapy | Year: 2010

Osteoarthritis is the most prevalent form of arthritis in the world. With the progressive ageing of the population, it is becoming a major public health problem. The involvement of certain signaling pathways, such as the Notch pathway, during cartilage pathology has been reported. In this review, we report on studies that investigated the expression pattern of the Notch family members in articular cartilage and the eventual involvement of this pathway in the modulation of the physiology and pathology of chondrocytes. Temporal and/or spatial modulation of this signaling pathway may help these cells to synthesize a new functional extracellular matrix and restore the functional properties of the articular cartilage. © 2011 BioMed Central Ltd. Source

Eya K.,Charles Nicolle Hospital | Bahlous A.,Charles Nicolle Hospital | Charni N.,Synarc | Bouzid K.,Charles Nicolle Hospital | And 6 more authors.
Clinical Laboratory | Year: 2012

Osteoarthritis is characterized by a progressive degeneration of articular cartilage and loss of joint function. Clinical assessment of osteoarthritis is hampered by the lack of accurate measures of disease and disease progresssion, especially during the early stage. Background: To investigate urinary C-telopeptide fragments of type II collagen (CTX-II) levels in knee osteoarthritis in the Tunisian population compared with controls and to assess the association between this biomarker and radiological signs. Methods: One hundred and twenty five female patients with knee osteoarthritis, aged 53.6 ±7.6 years with disease duration of 3.6 ±3.8 years and 57 female age-matched controls underwent Lyon Schuss X-ray exams. Two experienced readers independently measured the joint space width (JSW) and classified each knee for severity using the Kellgren/Lawrence scale. The urinary concentration of CTX-II was measured by a competitive ELISA. Results: The levels of urinary CTX-II were significantly higher in knee osteoarthritis patients compared with controls (323.98 vs 218.04 μg/mol creatinine). A weak and non significant association between the CTX-II level and JSW was found. The significant correlations were observed between age and CTX-II in both groups and between BMI and CTX-II only in controls. Conclusions: Analysis of CTX-II in urine samples of Tunisian patients with knee osteoarthritis provided a sensitive method to detect increased degradation of collagen type II in patients with osteoarthritis. Source

Kalai E.,Charles Nicolle Hospital | Bahlous A.,Charles Nicolle Hospital | Charni N.,Osteoarthritis Osteoporosis Research Laboratory | Bouzid K.,Charles Nicolle Hospital | And 9 more authors.
Joint Bone Spine | Year: 2012

Objectives: Proteolytic degradation of aggrecan is a hallmark of the pathology of osteoarthritis. The aim of this study was to develop enzyme-linked immunosorbent assay (ELISA) to quantify the serum levels of specific aggrecan fragments generated by aggrecanases-mediated cleavage. We investigated the relationships between these two aggrecan degradations fragments and urinary CTX-II levels. Methods: The competitive ELISAs employ a polyclonal antibody raised against the aggrecan fragments containing two neoepitopes NITEGE373and 374ARGSVI. We measured serum levels of ARGSV and NITEGE in 125 women with knee osteoarthritis (mean±SD age of 53.6±7.6 years, mean±SD disease duration of 3.6±3.8 years), and 57 women age-matched controls. Results: Aggrecan neoepitopes assays showed an intra- and inter-assay imprecision (CV) lower than 20% for both tests and good linearity. Median serum ARGSVI (by 18%; P= 0.002), and NITEGE (36.4%; P< 0.001) levels were significantly decreased in patients with knee osteoarthritis compared with controls. Minimal joint space width was negatively correlated with ARGSVI (r= -0.368, P= 0.04) and NITEGE (r= -0.274, P= 0.038) in knee osteoarthritis patients. Median urinary CTX-II levels were significantly increased by 39.5% (P= 0.001) in knee OA patients compared with controls. Conclusion: Markers of degradation aggrecan were analyzed for the first time in an African osteoarthritis population. These markers can be used to monitor aggrecanase activity in human joint disease. Their combination with CTX-II can improve clinical investigation of patients with osteoarthritis patients. © 2011 Société française de rhumatologie. Source

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