Ospedale Sacro Cuore Don Calabria

Negrar, Italy

Ospedale Sacro Cuore Don Calabria

Negrar, Italy

Time filter

Source Type

Torres A.,Medical University of Lublin | Torres K.,Medical University of Lublin | Torres K.,Lublin County Specialist Hospital | Pesci A.,Ospedale Sacro Cuore Don Calabria | And 5 more authors.
BMC Cancer | Year: 2012

Background: Alterations of mTOR gene expression have been implicated in the pathogenesis of endometrioid endometrial cancer however only few studies explored the cause of increased mTOR activation in this malignancy. miRNAs are small, noncoding RNAs, which were proven to regulated gene expression at the posttranscriptional level. The study aimed to explore deregulation of miRNAs targeting mTOR kinase (miR-99a, miR-100 and miR-199b) as a possible cause of its altered expression in EEC tissues. In addition expression of the three miRNAs was investigated in plasma of EEC patients and was assessed in terms of diagnostic and prognostic utility.Methods: We investigated expression of mTOR kinase transcripts in 46 fresh tissue samples. Expression of miR-99a, miR-100 and miR-199b was investigated in the same group of fresh samples, and in additional 58 FFPE sections as well as in 48 plasma samples using qPCR. Relative quantification was performed using experimentally validated endogenous controls.Results: mTOR kinase expression was increased in EEC tissues and was accompanied by decreased expression of all three miRNAs. Down-regulation of the investigated miRNAs was discovered in plasma of EEC patients and miRNA signatures classified EEC tissues (miR-99a/miR-100/miR-199b) and plasma (miR-99a/miR-199b) samples with higher accuracy in comparison to single miRNAs. We also revealed that miR-100 was an independent prognostic marker of overall survival.Conclusions: We conclude that increased expression of mTOR kinase coexists with down-regulation of its targeting miRNAs, which could suggest a new mechanism of mTOR pathway alterations in EEC. In addition, our findings implicate that miRNA signatures can be considered promising biomarkers for early detection and prognosis of endometrioid endometrial carcinoma. © 2012 Torres et al.; licensee BioMed Central Ltd.


Boschi F.,University of Verona | Pagliazzi M.,San Raffaele Scientific Institute | Rossi B.,University of Verona | Cecchini M.P.,University of Verona | And 3 more authors.
Journal of Biomedical Optics | Year: 2013

The in vitro and in vivo detection of visible photons from radioisotopes using optical techniques is a fastgrowing field in molecular imaging. 99mTc-pertechnetate is used as an alternative to 123I in imaging of the thyroid and is generally imaged with gamma cameras or single photon emission tomography instruments. The uptake in the thyroid tissue is mediated by the sodium-iodide symporter (NIS), a glycoprotein that actively mediates iodide transport into the thyroid follicular cells and several extrathyroidal tissues. The luminescence of the gamma emitter 99mTc-pertechnetate in order to visualize its biodistribution in healthy small living animals by using a commercial optical imaging system is investigated. Here we show that in Nu/Nu mice, the uptake of 99mTc-pertechnetate in the thyroid gland and in salivary glands is very detectable by using radionuclide luminescence imaging. We also found light emission from the stomach in accordance with the literature. The localization of the light signals in the anatomical regions where the radiopharmaceutical is expected, confirmed by resections, shows that it is possible to image NIS-expressing tissues. © Society of Photo-Optical Instrumentation Engineers.


PubMed | Ospedale Sacro Cuore Don Calabria, University of Padua, Volterra, University of Milan Bicocca and 5 more.
Type: | Journal: Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology | Year: 2017

Verbal reasoning is a complex, multicomponent function, which involves activation of functional processes and neural circuits distributed in both brain hemispheres. Thus, this ability is often impaired after brain injury. The aim of the present study is to describe the construction of a new verbal reasoning test (VRT) for patients with brain injury and to provide normative values in a sample of healthy Italian participants. Three hundred and eighty healthy Italian subjects (193 women and 187 men) of different ages (range 16-75years) and educational level (primary school to postgraduate degree) underwent the VRT. VRT is composed of seven subtests, investigating seven different domains. Multiple linear regression analysis revealed a significant effect of age and education on the participants performance in terms of both VRT total score and all seven subtest scores. No gender effect was found. A correction grid for raw scores was built from the linear equation derived from the scores. Inferential cut-off scores were estimated using a non-parametric technique, and equivalent scores were computed. We also provided a grid for the correction of results by z scores.


Partelli S.,MD. | Partelli S.,Marche Polytechnic University | Tamburrino D.,MD. | Crippa S.,Ph.D. | And 5 more authors.
American Journal of Surgery | Year: 2014

Background Amylase value in drains (AVD) is a predictor of pancreatic fistula (PF). We evaluated the accuracy of an AVD-based modelMethods: Two hundred thirty-one patients underwent pancreatoduodenectomy with pancreaticojejunostomy (PDPJ) or pancreatoduodenectomy with duct-to-mucosa (PDDTM) and distal pancreatectomy (DP). Patients with AVD greater than 5,000 U/L on postoperative day (POD) 1 underwent AVD measurement on POD5Results: Sensitivity and specificity of POD1 AVD greater than 5,000 in predicting PF were 71% and 90%, respectively. The sensitivity and specificity of POD5 AVD greater than 200 were 90% and 83%, respectively. AVD greater than 1,000 (for PDPJ) and 2,000 U/L (PDDTM and DP) represented the most accurate cutoffs on POD1. AVD greater than 200 (PDPJ), 300 (PDDTM), and 50 U/L (DP) represented the cutoffs with the highest sensitivity in predicting PF on POD5. Conclusion AVD-based model for predicting PF after pancreatic resection is an accurate tool, although AVD cutoffs should be evaluated for each type of operation. © 2014 Elsevier Inc.


Zamboni G.,University of Verona | Hirabayashi K.,Ospedale Sacro Cuore Don Calabria | Hirabayashi K.,Tokai University | Castelli P.,Ospedale Sacro Cuore Don Calabria | Lennon A.M.,Johns Hopkins Hospital
Best Practice and Research: Clinical Gastroenterology | Year: 2013

Pancreatic cancer has a very poor prognosis, with a five year survival of only 5%. New studies have shown that it takes over 11 years for cells to develop invasive capability. This provides an opportunity to intervene if precursor lesions can be detected. This paper reviews the molecular, pathological, clinical findings and management of pancreatic intraepithelial neoplasia (PanIN), intraductal pancreatic mucinous neoplasm (IPMN) and mucinous cystic neoplasm (MCN), three precursor lesions which can give rise to invasive carcinoma of the pancreas. © 2013 Elsevier Ltd. All rights reserved.


Hirabayashi K.,Ospedale Sacro Cuore Don Calabria | Hirabayashi K.,Tokai University | Zamboni G.,Ospedale Sacro Cuore Don Calabria | Zamboni G.,University of Verona | And 4 more authors.
Frontiers in Oncology | Year: 2013

Gastrointestinal neuroendocrine neoplasms (GI-NENs) arise from neuroendocrine cells distributed mainly in the mucosa and submucosa of the gastrointestinal tract. In 2010, the World Health Organization (WHO) classification of NENs of the digestive system was changed, categorizing these tumors as grade 1 neuroendocrine tumor (NET), grade-2NET, neuroendocrine carcinoma (large- or small-cell type), or mixed adenoneuroendocrine carcinoma (MANEC). Such a classification is based on the Ki-67 index and mitotic count in histological material. For the accurate pathological diagnosis and grading of NENs, it is important to clearly recognize the characteristic histological features of GI-NENs and to understand the correct method of counting Ki-67 and mitoses. In this review, we focus on the histopathological features of GI-NENs, particularly regarding biopsy and cytological diagnoses, neuroendocrine markers, genetic and molecular features, and the evaluation of the Ki-67 index and mitotic count. In addition, we will address the histological features of GI-NEN in specific organs.© 2013 Hirabayashi, Zamboni, Nishi, Tanaka, Kajiwara and Nakamura.


Hirabayashi K.,Ospedale Sacro Cuore Don Calabria | Hirabayashi K.,Tokai University | Zamboni G.,Ospedale Sacro Cuore Don Calabria | Zamboni G.,University of Verona
Pathologica | Year: 2012

IgG4-related disease (IgG4-RD) is considered a fibro-inflammatory condition with a marked propensity to form mass forming lesions, characterized by a dense lymphoplasmacytic infiltrate, the presence of abundant IgG4+ plasma cells, frequent elevation of serum IgG4 and a dramatic initial response to glucocorticoid. Nowadays, IgG4-RD has been described in almost every organ system: the pancreatobiliary tract, liver, salivary glands, nasopharynx, bone marrow, lacrimal gland, extra-ocular muscles and retrobulbar space, kidneys, lungs, lymph nodes, meninges, aorta and arteries, skin, breast, prostate, thyroid gland and pericardium. Although the common diagnostic features of all these regional involvements cannot be defined with certainty, and slight differences have been noted in different organs, many histopathological features are shared. Consensus has not yet been reached regarding criteria that have to be fulfilled for a new IgG4-RD. The proposed criteria include appropriate clinical and histopathological findings, presence of abundant tissueinfiltrating IgG4+ plasma cells, high serum IgG4 concentrations, response to steroid therapy, other autoimmune diseases or other organ involvement. The two hallmark features for diagnosis are histopathological characteristics and the presence of infiltrating IgG4+ plasma cells. In this review, we will focus on the histopathological features of IgG4-RD in specific organs and discuss the relationship with inflammatory pseudotumour and malignancy, IgG4 counting methods, and diagnosis using biopsy specimens. • IgG4-related disease (IgG4-RD) is a multi-organ system disease that has been recognized in the last 10 years. • IgG4-RD has a marked propensity to present as mass-forming lesions. • The two hallmark features for diagnosis are histopathological characteristics and the presence of infiltrating IgG4+ plasma cells. • Correct identification is crucial to avoid unnecessary major surgical procedures and initiate corticosteroid therapy.


Cirillo M.,Ospedale Sacro Cuore Don Calabria | Musola M.,Obstetrics and Gynecology Unit | Cassandrini P.A.,Ospedale Sacro Cuore Don Calabria | Lunardi G.,Ospedale Sacro Cuore Don Calabria | Venturini M.,Ospedale Sacro Cuore Don Calabria
Tumori | Year: 2012

Background. Colon cancer during pregnancy is a relatively rare occurrence. To date there has been sparse clinical evidence about the safety of chemotherapy in this setting because the available data derive only from single-institution case reports. Methods. Irinotecan and fluorouracil, as part of the FOLFIRI regimen, were administered to a 33-year-old pregnant woman at an estimated gestational age of 23+ weeks. She had been diagnosed with adenocarcinoma of the transverse colon with liver and lymph node metastases. Results. Chemotherapy was administered from the 23+th to the 28+th week of gestational age. Chemotherapy was stopped because of disease progression. At 30 weeks' gestational age, the patient underwent an emergency cesarean section and colon resection. She gave birth to a healthy male infant with no particular problems in neurological, respiratory, cardiovascular, digestive and nutritional function. At follow-up, the 13-monthold child had achieved all appropriate growth and developmental milestones. Conclusions. Our report demonstrates the safety of exposure to FOLFIRI for both mother and fetus. The absence of any abnormalities in the infant makes irinotecan and fluorouracil a valid therapeutic option for colon cancer during pregnancy. © II Pensiero Scientifico Editore downloaded by EXCERPTA MEDICA.


Mete M.,Ospedale Sacro Cuore Don Calabria | Parolini B.,Ospedale Sacro Cuore Don Calabria | Maggio E.,Ospedale Sacro Cuore Don Calabria | Pertile G.,Ospedale Sacro Cuore Don Calabria
Graefe's Archive for Clinical and Experimental Ophthalmology | Year: 2011

Background: Several surgical techniques have been described for the treatment of retinal detachment (RD) associated to myopic macular hole (MMH). In this retrospective study, the anatomical and functional outcomes of pars plana vitrectomy (PPV) with long-term tamponade, using either 1000 cSt silicone oil (SO) or heavy silicone oil (HSO), are compared. Methods: Forty-two eyes affected by RD associated with MMH were included. The surgical technique involved standard 3-port 20-gauge PPV with long-term tamponade. The patients were divided into two groups, according to the intraocular tamponade: SO in group 1 (n=17), and HSO in group 2 (n=25). Internal limiting membrane (ILM) removal was performed in 15 cases of group 1 and 20 cases of group 2. Tamponade removal was performed 2 to 5 months after primary surgery. The patients were assessed 1 week and 1 month after primary surgery, and then 1 week and 1 month after tamponade removal or after further surgery if macular redetachment had occurred. The patients were also visited every 2 months for at least 1 year after final tamponade removal. Follow-up was considered closed at 1 year after final tamponade removal. Results: Preoperative best-corrected visual acuity (BCVA), expressed as LogMar, was 2.8±0.77 for group 1 and 2.1±0.94 for group 2. At the last visit, the BCVA was 1.41±0.96 and 1.48±0.77 for groups 1 and 2 respectively. Retinal reattachment was achieved with one operation in 13 eyes of group 1 (76.5%) and 18 of group 2 (81.8%) (P=0.69). The average number of surgery needed to achieve retinal attachment by patients of group 1 and 2 was respectively 1.36±0.63 and 1.46±0.59 (P=0.77). Five patients of group 1 and four of group 2 developed a chronic glaucoma (P=0.238). Conclusions: PPV with ILM peeling and long-term tamponade was demonstrated to be a good surgical option to treat RD due to MMH; SO and HSO seemed to be equally effective, although the success rates remained far from an ideal 100%. © 2010 Springer-Verlag.

Loading Ospedale Sacro Cuore Don Calabria collaborators
Loading Ospedale Sacro Cuore Don Calabria collaborators