Osong Health Technology Administration Complex

Mungyeong, South Korea

Osong Health Technology Administration Complex

Mungyeong, South Korea
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Hwang C.J.,Chungbuk National University | Yun H.-M.,Chungbuk National University | Jung Y.Y.,Chungbuk National University | Lee D.H.,Chungbuk National University | And 8 more authors.
Molecular Neurobiology | Year: 2015

Neuroinflammation is important for the development of several neurodegenerative diseases such as Alzheimer’s disease, Parkinson’s disease, and stroke. Since changes of cytokine level are critical for neuroinflammation in the brain, we investigated whether IL-32α overexpression could change neuroinflammation and, thus, affect stroke development. Middle cerebral artery occlusion (MCAO) induced development of ischemia, and ischemic neuronal cell death were reduced in IL-32α-overexpressing transgenic mice (IL-32α mice) brain through the decreased release of neuroinflammatory cytokines (IL-6, IL-1β, TNF-α) and activation of astrocytes, but enhancement of anti-neuroinflammatory cytokines (IL-10). Reactive oxygen species generation and lipid peroxidation as well as expression of inducible nitric oxide and cyclooxygenase-2 were also reduced in the IL-32α mice brain. Nuclear factor-kappa B (NF-κB), a critical transcriptional factor regulating neuroinflammation, was much lower, but activation of signal transducer and activator of transcription 3 (STAT3), which plays a crucial role in cell survival and proliferation, was much higher in IL-32α-overexpressing mice brain compared to those of wild-type mice brain. These results suggest that IL-32α can prevent cerebral ischemia damage via upregulation of anti-neuroinflammatory cytokine expression and STAT3 activation, but downregulation of neuroinflammatory cytokines and NF-κB activation. © 2014, Springer Science+Business Media New York.


Lee B.-R.,Osong Health Technology Administration Complex | Lee J.-H.,Dongduk Women's University | An H.-J.,Sangji University
Molecules | Year: 2012

In Korean herbal medicine dandelion (Taraxacum officinale, TO) has been used to improve energy levels and health. However, the effects of TO in experimental models remain unclear. We examined the anti-fatigue and immune-enhancing effects of TO in mice by performing a forced swimming test (FST) and in vitro by using peritoneal macrophages, respectively. After daily oral administration of TO, blood biochemical parameters related to fatigue were measured after the FST. FST immobility time was significantly decreased in the TO-treated group (100 mg/kg) on the tenth day. TO (10 and 100 mg/kg) treatment significantly increased glucose levels, acting as an energy source. The level of lactic dehydrogenase, which is an accurate indicator of muscle damage, tended to decline after TO administration (10 and 100 mg/kg). When TO (100 mg/kg) was orally administered to mice, blood urea nitrogen levels decreased significantly. We also examined the effect of TO on the production of cytokines and nitric oxide (NO) in mouse peritoneal macrophages. When TO was used in combination with recombinant interferon-gamma (rIFN-γ), a noticeable cooperative induction of tumor necrosis factor-alpha (TNF-α), interleukin (IL)-12p70, and IL-10 production was observed. Furthermore, in peritoneal macrophages, rIFN-γ plus TO treatment significantly increased the production of NO through inducible nitric oxide synthase (iNOS) induction. Taken together, these results suggest that TO improves fatigue-related indicators and immunological parameters in mice. © 2012 by the authors.


Son M.-H.,Pohang University of Science and Technology | Kim J.-T.,Pohang University of Science and Technology | Park H.,Pohang University of Science and Technology | Kim M.,Osong Health Technology Administration Complex | And 2 more authors.
Chemosphere | Year: 2012

The dietary intake of polychlorinated biphenyls (PCBs) was estimated using the sum of 62 PCB congeners (∑62PCBs), including seven indicator PCBs and 12 dioxin-like PCBs, in the South Korea. In this study, 200 individual food samples belonging to 40 different foodstuffs were investigated to estimate the distribution of PCB congeners in five sampling cities. PCB exposure was estimated using Korean dietary habits as established by the National Health and Nutrition Examination Survey (NHANES). The PCB concentrations in rice, the most frequently consumed food in Korea, was relatively low in whole food samples. The mean PCB levels measured in fish were the highest in this study, but each fish is consumed in relatively small amounts by the general population. Therefore, the daily dietary intake should also be considered with regard to human exposure to PCBs, especially with the consumption of contaminated foods. Dioxin-like PCB levels were also calculated using TEF values that were established in 2005. The average levels (pg TEQ/g) were 0.0002 for rice and 0.0098 for fish. The dioxin-like PCBs accounted for a relatively small percentage of the total PCBs, compared to previous studies. According to our research, the health risks associated with exposure to PCBs could be estimated using the tolerable daily intake (TDI) of the general population. © 2012 Elsevier Ltd.


Kim J.,Pohang University of Science and Technology | Son M.-H.,Pohang University of Science and Technology | Suh J.,Osong Health Technology Administration Complex | Kang Y.,Osong Health Technology Administration Complex | Chang Y.-S.,Pohang University of Science and Technology
Journal of Hazardous Materials | Year: 2014

A total of 175 samples, composed of 35 different foodstuffs, were obtained from retail markets in Korea and analyzed to investigate the contamination status and dietary intake of Dechlorane compounds. The concentrations of Dechlorane Plus (DP), mirex, Dechlorane (Dec) 602 and 603 ranged from ND to 169.85pg/g wet weight (ww), 107.30pg/gww, 20.81pg/gww, 0.41pg/gww, respectively, while Dechlorane (Dec) 604 was not detected in any samples. Strong correlations between anti- and syn-DP and between mirex and Dec 602 were observed. The average anti-isomer fractional abundance (fanti) was 0.78±0.11 which was larger than the technical DP value (fanti=0.75). The estimated dietary daily intake of DP, 11.2×103pg/day, was one to three orders of magnitude higher than other Dechloranes. Grain was the most contributed food group to dietary daily intake of DP for Korean population. © 2014 Elsevier B.V.


Lim H.-S.,Osong Health Technology Administration Complex | Choi J.-C.,Osong Health Technology Administration Complex | Song S.-B.,Osong Health Technology Administration Complex | Kim M.,Osong Health Technology Administration Complex
Food Chemistry | Year: 2014

A simple and rapid method has been developed and validated for the determination of carmine in foods. Samples were homogenised and extracted with 0.05 M NaOH, followed by centrifugation. The resulting solution was filtered and injected to HPLC. Carmine was separated by HPLC using an NovaPak C18 column coupled to a photodiode array detector. The contents of carmine were finally quantified using corresponding calibration curves over ranges of 1.0-100 μg ml-1, with good correlation coefficients (r2 = 0.9999). The recoveries of carmine from foods spiked at levels of 10, 50, and 100 μg g-1 which ranged from 90.4% to 96.2% with relative standard deviations between 2.8% and 6.8%. Limit of detection and limit of quantification of carmine were 0.4 and 1.0 μg ml-1, respectively. This method was found to be useful to distinguish carmine from carminic acid, a major component of cochineal extract. The method has been successfully applied to various foods. © 2014 Elsevier Ltd. All rights reserved.


Scarr E.,University of Melbourne | Um J.Y.,University of Melbourne | Um J.Y.,Osong Health Technology Administration Complex | Cowie T.F.,University of Melbourne | Dean B.,University of Melbourne
Schizophrenia Research | Year: 2013

Although schizophrenia is a widespread disorder of unknown aetiology, we have previously shown that muscarinic M4 receptor (CHRM4) expression is decreased in the hippocampus and caudate-putamen from subjects with the disorder, implicating the receptor in its pathophysiology. These findings led us to determine whether variation in the CHRM4 gene sequence was associated with an altered risk of schizophrenia by sequencing the CHRM4 gene from the brains of 76 people with the disorder and 74 people with no history of psychiatric disorders. In addition, because the CHRM4 is a potential target for antipsychotic drug development, we investigated whether variations in CHRM4 sequence were associated with final recorded doses of, and life-time exposure to, antipsychotic drugs. Gene sequencing identified two single nucleotide polymorphisms (SNPs; rs2067482 and rs72910092) in the CHRM4 gene. For rs2067482, our data suggested that both genotype (1341C/C; p = 0.05) and allele (C; p = 0.03) were associated with an increased risk of schizophrenia. In addition, there was a strong trend (p = 0.08) towards an association between CHRM4 sequence and increased lifetime exposure to antipsychotic drugs. Furthermore, there was a trend for people with the C allele to be prescribed benzodiazepines more frequently (p = 0.06) than those with the T allele. These data, albeit on small cohorts, are consistent with genetic variance at rs2067482 contributing to an altered risk of developing schizophrenia which requires more forceful pharmacotherapy to achieve a clinical response. © 2013 Elsevier B.V.


Lee J.B.,Osong Health Technology Administration Complex | Kim M.,Osong Health Technology Administration Complex
Food Science and Biotechnology | Year: 2012

Ultraviolet (UV) light induced photo-degradation of malachite green (MG) was investigated in the absence of photocatalysts. Photo-degraded intermediates were identified using LC with visible detection and LCelectrospray ionization-MS. Using this analysis, mechanisms for the MG decomposition in aqueous solution and in mudfish tissue were suggested. In aqueous condition, N-de-methylation and the decomposition of the conjugated structures were mainly observed. On the other hand, MG levels in mudfish continuously decreased until they reached a plateau owing to the decomposition of the conjugated structures. These results show that some chemicals in fish tissues could be photo-degraded by UV light without photocatalysts. © 2012 The Korean Society of Food Science and Technology and Springer Netherlands.


Kim N.S.,Osong Health Technology Administration Complex | Lee J.H.,Osong Health Technology Administration Complex | Han K.M.,Osong Health Technology Administration Complex | Kim J.W.,Osong Health Technology Administration Complex | And 2 more authors.
Food Chemistry | Year: 2014

In this study, a method for discriminating natural mozzarella cheese from cheese substitutes, using fatty acid profiles, phytosterol contents, and statistical comparison, was developed. A total of 27 cheeses were evaluated: eight natural mozzarella cheeses (NMCs), four imitation mozzarella cheeses (IMCs), 12 processed cheeses (PCs) and three mixed cheeses (MCs) composed of NMCs and IMCs. The fatty acid composition of the NMC class was distinct from those of the IMC and MC classes, but statistically similar (p < 0.05) to that of the PC class. The phytosterol content of the NMC class, determined via gas chromatography- mass spectrometry, was distinct from the IMCs, but similar (p < 0.05) to a portion of the PCs. Principal component analysis (eigenvalue P1) indicated that the NMCs can be differentiated from the IMCs, but discrimination between the NMCs and the PCs could not be achieved. © 2013 Elsevier Ltd.


Seo H.-O.,Osong Health Technology Administration Complex | Sohn Y.-T.,Duksung Womens University
Journal of Thermal Analysis and Calorimetry | Year: 2014

DA-6034 is a synthetic flavonoid known to possess anti-inflammatory activity. DA-6034 has been found to exist in six crystal forms, one hydrate form and five solvates. Six crystal forms, one hydrate form and five solvates, of DA-6034 were prepared by recrystallization and they were characterized by DSC, TG and PXRD (Shin and Sohn, J Therm Anal Calorim 115:2457-2461, 2014). This research aimed at investigating the transformation of crystal forms of DA-6034 depending on temperature and relative humidity. Six crystal forms of DA-6034 were kept in different conditions: in 0, 52, and 95 % RH at room temperature to investigate the effect of relative humidity and at RT, 40, and 60 °C to investigate that of temperature. PXRD and TG were used to examine the state of modifications. At 0 % RH Form 2, Form 3, and Form 5 were transformed to amorphous solid and Form 6 was not transformed and Form 4 was transformed to anhydrate. At 52, 95 % RH Form 2, Form 3, Form 4, and Form 6 were transformed to Form 1 (monohydrate) and Form 5 (1.5 H2O, 1/2 acetic acid solvate) was transformed to Form 8 (2 H2O, 0.15 acetic acid solvate). © 2014 Akadémiai Kiadó, Budapest, Hungary.


Son K.H.,Osong Health Technology Administration Complex | Heo M.Y.,Kangwon National University
International Journal of Cosmetic Science | Year: 2013

Synopsis In this review, the evaluation methods for the screening of depigmenting substrates were investigated. For this purpose, the evaluation method of tyrosinase, a key enzyme of melanin biosynthesis, is most frequently used, but evaluating methods based on the regulation of cellular signal transfer factors or the inhibition of melanosome transfer have also been developed. Evaluation of the depigmenting effect using melanocytes is complex. It has the advantage of being capable of analysing overall effects on melanin biosynthesis at cellular levels. Before the final clinical testing of depigmenting agents, in vitro testing should be conducted to confirm the depigmenting efficacy and safety. Clinical studies for depigmenting agents can be used to investigate the prevention of melanin biosynthesis and to determine whether melanin disappears from skin. Therefore, the most appropriate protocol has to be employed, depending on the mechanism of action of the depigmenting agent. © 2012 Society.

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