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Dasgupta S.,Indian Statistical Institute | Sirisha P.V.S.,Indian Statistical Institute | Neelaveni K.,Osmania General Hospital | Anuradha K.,Anu Test Tube Baby Center | Reddy B.M.,Indian Statistical Institute
PLoS ONE | Year: 2012

Polycystic Ovary Syndrome (PCOS) is known to be characterized by metabolic disorder in which hyperinsulinemia and peripheral insulin resistance are central features. Given the physiological overlap between PCOS and type-2 diabetes (T2DM), and calpain 10 gene (CAPN10) being a strong candidate for T2DM, a number of studies have analyzed CAPN10 SNPs among PCOS women yielding contradictory results. Our study is first of its kind to investigate the association pattern of CAPN10 polymorphisms (UCSNP-44, 43, 56, 19 and 63) with PCOS among Indian women. 250 PCOS cases and 299 controls from Southern India were recruited for this study. Allele and genotype frequencies of the SNPs were determined and compared between the cases and controls. Results show significant association of UCSNP-44 genotype CC with PCOS (p = 0.007) with highly significant odds ratio when compared to TC (OR = 2.51, p = 0.003, 95% CI = 1.37-4.61) as well as TT (OR = 1.94, p = 0.016, 95% CI = 1.13-3.34). While the haplotype carrying the SNP-44 and SNP-19 variants (21121) exhibited a 2 fold increase in the risk for PCOS (OR = 2.37, p = 0.03), the haplotype containing SNP-56 and SNP-19 variants (11221) seems to have a protective role against PCOS (OR = 0.20, p = 0.004). Our results support the earlier evidence for a possible role of UCSNP-44 of the CAPN10 gene in the manifestation of PCOS. © 2012 Dasgupta et al. Source


Dasgupta S.,Indian Statistical Institute | Sirisha P.V.S.,Indian Statistical Institute | Neelaveni K.,Osmania General Hospital | Anuradha K.,Anu Test Tube Baby Center | And 3 more authors.
PLoS ONE | Year: 2010

The present study was carried out to assess the role of androgen receptor CAG repeat polymorphism and X chromosome inactivation (XCI) pattern among Indian PCOS women and controls which has not been hitherto explored and also to test the hypothesis that shorter CAG alleles would be preferentially activated in PCOS. CAG repeat polymorphism and X chromosome methylation patterns were compared between PCOS and non-PCOS women. 250 PCOS women and 299 controls were included for this study. Androgen receptor CAG repeat sizes, XCI percentages, and clinical and biochemical parameters were measured. The mean CAG repeat number is similar between the cases (18.746±0.13) and controls (18.736±0.12). The obese PCOS women were significantly more frequent in the, <18 and > 20 CAG repeat category than the lean PCOS women, yielding a highly significant odds (p = 0.001). Among the women with non-random X-inactivation, alleles with <19 repeats were more frequently activated among cases than controls (p = 0.33). CAG repeat polymorphism by itself cannot be considered as a useful marker for discriminating PCOS. We observed a trend of preferential activation of the shorter allele among the PCOS cases with non random XCI pattern. In the obese PCOS women, this microsatellite variation may account for the hyperandrogenicity to a larger extent than the lean PCOS women.© 2010 Dasgupta et al. Source


Ramakrishnan D.,Osmania General Hospital
Journal of Clinical and Diagnostic Research | Year: 2016

Introduction: The principle behind the generation of the Korotkoff sounds is the turbulence of blood flowing through a partially occluded area in the artery. With increasing age, the vascular wall compliance is expected to decrease, which is due to the thickening of the vessel wall, due to which the amplitude of the transmitted Korotkoff sounds is decreased. There is also an accompanying rise in the systolic B.P. and pulse pressure. Aim: To record and compare the amplitudes of the intermediate Korotkoff sounds and the blood pressures in individuals of the two age groups, and calculate the pulse pressure and determine whether they vary in relation to the amplitude of the intermediate Korotkoff sounds recorded. Materials and Methods: The cross-sectional study was conducted on 50 young subjects (15-25 years) and 50 older subjects (50-70 years). The mid arm circumference was measured using a tape. A phonoarteriogram was placed over the left brachial artery and the sphygmomanometer cuff was tied 2cm above the cubital fossa of the left arm. The blood pressure was recorded using the Lab Tutor software. The Korotkoff sounds picked up and transmitted by the phonoarteriogram are represented as distinct lines on the graphical recording. Statistical Analysis: Independent samples t-test to look for significant mean amplitude differences and for correlating mean amplitude and pulse pressure. Null hypothesis rejected at p<0.05. Data analysed using the SPSS software version 20.0 (SPSS Inc.). Results: There was a significant difference in the mean amplitudes of Korotkoff sounds among the different age groups (p=0.001) and subject categories (p=0.043 among males, p=0.037 among females). A significant difference in pulse pressures was also seen among different age groups and subject categories. The decrease in the amplitudes of Korotkoff sounds in the older age group accompanies the increase in pulse pressures seen in this group and the same was seen among the different age groups within each sex (r=-0.574, p=0.001 among males) and (r=-0.449, p=0.002 among females). Conclusion: Measuring the amplitude of Korotkoff sounds can give us an idea of the nature of the arterial wall as shown by a decrease in amplitude in older individuals when compared to the younger individuals and of the volume of blood flowing through the artery as shown by the higher amplitudes observed in males when compared to females. © 2016, Journal of Clinical and Diagnostic Research. All rights reserved. Source


Pantula N.R.,Kamineni Hospitals | Vedula K.,Osmania General Hospital
Asian Cardiovascular and Thoracic Annals | Year: 2014

Heparin-induced thrombocytopenia is a rare but serious and life-threatening complication of heparin use. We describe the case of a 7-year-old girl with tetralogy of Fallot with pulmonary atresia, who developed multiple thrombotic complications following intracardiac repair, requiring below elbow amputation of the left forearm and right forefoot. Our case illustrates the need for greater awareness of heparin-induced thrombocytopenia, to prevent the significant morbidity associated with it. © The Author(s) 2013 Reprints and permissions: sagepub.co.uk/journalsPermissions.nav. Source


Sahay M.,Osmania General Hospital
Saudi journal of kidney diseases and transplantation : an official publication of the Saudi Center for Organ Transplantation, Saudi Arabia | Year: 2013

The aim of this study is to review cases of renal cortical necrosis (RCN) that were seen on renal biopsy at our center over a period of seven years. All renal biopsy records over seven years at the Osmania General Hospital were reviewed to identify patients with histologically proven RCN. The demographic, clinical, laboratory investigations and follow-up data were noted. There were a total of 105 patients with RCN. The mean age was 28.13 ± 12.40 years. Forty-one cases (39.04%) resulted from obstetric complications. The most common histology type of RCN was patchy cortical necrosis in 65 patients (62%). All patients required dialysis, and the mean duration of dialysis was 3 ± 1 weeks. Thirty-three (31.42%) patients progressed to end-stage kidney disease while three patients underwent renal transplantation. Ten (9.5%) patients succumbed to acute kidney injury. The remaining patients recovered sufficient renal function and were dialysis-independent till the last follow-up. RCN is an important cause of chronic kidney disease. Obstetric complications are the leading cause of RCN in our setting. An early renal biopsy, especially in cases of anuric renal failure, helps in establishing the diagnosis. Source

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