Osijek Agricultural Institute

Osijek, Croatia

Osijek Agricultural Institute

Osijek, Croatia
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Spanic V.,Osijek Agricultural Institute | Viljevac Vuletic M.,Osijek Agricultural Institute | Abicic I.,Osijek Agricultural Institute
Plant Physiology and Biochemistry | Year: 2017

Fusarium head blight (FHB) is a destructive fungal disease of wheat (Triticum aestivum L.) that causes significant grain yield losses and end-use quality reduction associated with contamination by the mycotoxin deoxynivalenol (DON). Three winter wheat varieties (‘Vulkan’, ‘Kraljica’ and ‘Golubica’) were screened for FHB resistance using artificial inoculation technique under field conditions. The aim of this study was to examine a relationship between FHB resistance and the effectiveness of enzyme antioxidant system of wheat varieties under different sampling times (3, 15, 24, 48, 96, 120 and 336 hai). In the time-course experiments FHB-resistant variety ‘Vulkan’ showed rapid induction of ascorbate peroxidase (APX) and polyphenol oxidase (PPO) activity in the early stages after infection (3 hai) and it seems that in ‘Vulkan’ FHB-resistance is associated with antioxidative enzymes activity. Moderately FHB resistant variety ‘Kraljica’ showed the higher guaiacol peroxidase (POD) activity and higher H2O2 content after 24 hai, increased malondialdehyde (MDA) content at the beginning of infection (3, 15 hai) while induction of catalase (CAT), APX and PPO was delayed. FHB-susceptible variety ‘Golubica’ involved antioxidant enzymes in defense response much later. Based on our results the activity of antioxidant enzymes (APX and PPO) was more pronounced in ‘Vulkan’ than in FHB-medium resistant variety ‘Kraljica’ and FHB-susceptible ‘Golubica’. The differences in antioxidant response of wheat varieties under Fusarium infestation could be the result of genetic properties. © 2017 Elsevier Masson SAS


Vojta L.,Ruder Boskovic Institute | Caric D.,Ruder Boskovic Institute | Cesar V.,Josip Juraj Strossmayer University of Osijek | Antunovic J.,Josip Juraj Strossmayer University of Osijek | And 3 more authors.
Scientific Reports | Year: 2015

In photosynthesis, final electron transfer from ferredoxin to NADP + is accomplished by the flavo enzyme ferredoxin:NADP + oxidoreductase (FNR). FNR is recruited to thylakoid membranes via integral membrane thylakoid rhodanase-like protein TROL. We address the fate of electrons downstream of photosystem I when TROL is absent. We have employed electron paramagnetic resonance (EPR) spectroscopy to study free radical formation and electron partitioning in TROL-depleted chloroplasts. DMPO was used to detect superoxide anion (O 2.-) formation, while the generation of other free radicals was monitored by Tiron. Chloroplasts from trol plants pre-acclimated to different light conditions consistently exhibited diminished O 2.- accumulation. Generation of other radical forms was elevated in trol chloroplasts in all tested conditions, except for the plants pre-acclimated to high-light. Remarkably, dark- and growth light-acclimated trol chloroplasts were resilient to O 2.- generation induced by methyl-viologen. We propose that the dynamic binding and release of FNR from TROL can control the flow of photosynthetic electrons prior to activation of the pseudo-cyclic electron transfer pathway.


Simic D.,Osijek Agricultural Institute | Jurkovic V.,Osijek Agricultural Institute | Antunovic J.,Josip Juraj Strossmayer University of Osijek | Cesar V.,Josip Juraj Strossmayer University of Osijek
Journal of Integrative Plant Biology | Year: 2014

Chlorophyll fluorescence transient from initial to maximum fluorescence ("P" step) throughout two intermediate steps ("J" and "I") (JIP-test) is considered a reliable early quantitative indicator of stress in plants. The JIP-test is particularly useful for crop plants when applied in variable field environments. The aim of the present study was to conduct a quantitative trait loci (QTL) analysis for nine JIP-test parameters in maize during flowering in four field environments differing in weather conditions. QTL analysis and identification of putative candidate genes might help to explain the genetic relationship between photosynthesis and different field scenarios in maize plants. The JIP-test parameters were analyzed in the intermated B73×Mo17 (IBM) maize population of 205 recombinant inbred lines. A set of 2,178 molecular markers across the whole maize genome was used for QTL analysis revealing 10 significant QTLs for seven JIP-test parameters, of which five were co-localized when combined over the four environments indicating polygenic inheritance and pleiotropy. Our results demonstrate that QTL analysis of chlorophyll fluorescence parameters was capable of detecting one pleiotropic locus on chromosome 7, coinciding with the gene gst23 that may be associated with efficient photosynthesis under different field scenarios. © 2014 Institute of Botany, Chinese Academy of Sciences.


Spanic V.,Osijek Agricultural Institute | Lemmens M.,Institute of Biotechnology in Plant Production | Drezner G.,Osijek Agricultural Institute
European Journal of Plant Pathology | Year: 2010

Fusarium head blight (FHB) is an important fungal disease of wheat. The aim of this research was to determine the diversity of Fusarium species infecting winter wheat ears in East Croatia. In 2008 wheat kernels were obtained from three locations in the eastern part of Croatia (Tovarnik, Osijek, Pozega), and in 2009 from two additional locations (Slavonski Brod, Nova Gradiska). In total, 498 visually diseased kernels were selected for morphological identification of Fusarium spp. The identity of 226 selected isolates was further investigated by molecular techniques. The predominant species on wheat kernels in East Croatia in 2008 were F. graminearum, isolated and confirmed from more than 80% of sampled wheat kernels, followed by F. avenaceum (8%) and F. culmorum (7%). Incidence of F. poae was less than 2%. The most common species identified in 2009 were F. graminearum (50%), F. culmorum (13%), F. avenaceum (12%) and F. poae (7%). This is the first report on the identification of Fusarium species isolated from naturally infected wheat ears in Croatia. © 2010 KNPV.


Simic D.,Osijek Agricultural Institute | Mladenovic Drinic S.,Maize Research Institute Zemun Polje | Zdunic Z.,Maize Research Institute Zemun Polje | Jambrovic A.,Osijek Agricultural Institute | And 4 more authors.
Journal of Heredity | Year: 2012

Detecting genes that influence biofortification traits in cereal grain could help increase the concentrations of bioavailable mineral elements in crops to solve the global mineral malnutrition problem. The aims of this study were to detect the quantitative trait loci (QTLs) for phosphorus (P), iron (Fe), zinc (Zn), and magnesium (Mg) concentrations in maize grain in a mapping population, as well as QTLs for bioavailable Fe, Zn, and Mg, by precalculating their respective ratios with P. Elemental analysis of grain samples was done by coupled plasma-optical emission spectrometry in 294 F4 lines of a biparental population taken from field trials of over 3 years. The population was mapped using sets of 121 polymorphic markers. QTL analysis revealed 32 significant QTLs detected for 7 traits, of which some were colocalized. The Additive-dominant model revealed highly significant additive effects, suggesting that biofortification traits in maize are generally controlled by numerous small-effect QTLs. Three QTLs for Fe/P, Zn/P, and Mg/P were colocalized on chromosome 3, coinciding with simple sequence repeats marker bnlg1456, which resides in close proximity to previously identified phytase genes (ZM phys1 and phys2). Thus, we recommend the ratios as bioavailability traits in biofortification research. © The American Genetic Association. 2011. All rights reserved.


Radic S.,University of Zagreb | Peharec Stefanic P.,University of Zagreb | Lepedus H.,Osijek Agricultural Institute | Roje V.,University of Zagreb | Pevalek-Kozlina B.,University of Zagreb
Environmental and Experimental Botany | Year: 2013

The present study investigated the effects of salinity on the perennial species Centaurea ragusina L. interesting as a potential cash crop plant. Plants grown in culture conditions were subjected to increasing salt (0-600. mM NaCl) or mannitol (300. mM) treatments for two weeks. Effects of isoosmotic concentrations of NaCl (150. mM) and mannitol were compared and discussed in order to discriminate possible differences in C. ragusina response to ionic (NaCl) and osmotic (mannitol) components of salinity. C. ragusina plants used Na and to a lesser extent Cl ions as a primary osmotica though with higher salinity, proline accumulation increased as well. Concurrently, with increasing salinity significant reductions in plant K, Mg and Ca concentrations occurred. In addition, lower salt concentrations induced leaf succulence and increased leaf relative water content (RWC). A threshold salinity above which C. ragusina L. showed signs of damage and growth inhibition was reached at 300. mM. Activities of superoxide dismutase, catalase and ascorbate peroxidase in salinized plants seem to play an essential protective role in the scavenging processes. Regardless of the high induction of antioxidative system and massive proline accumulation, mannitol caused decrease of RWC and oxidative damage to proteins and lipids. Considering the abundance of some photosynthetic proteins (Rubisco, D1, LHCI, LHII and FNR) and PSII efficiency, it can be concluded that both salt and mannitol impaired photosynthesis in C. ragusina though salt to a much lesser extent. The results suggest that the major reason for the particular threshold of salinity tolerance in C. ragusina can be attributed to limited dilution capacity of succulent tissue. The tolerance strategies of C. ragusina to moderate salinity seem to include osmotic adjustment achieved through salt ions uptake as a dominant strategy but also highly inducible antioxidative defense. © 2012 Elsevier B.V.


Andric L.,Osijek Agricultural Institute | Rastija M.,Josip Juraj Strossmayer University of Osijek | Teklic T.,Josip Juraj Strossmayer University of Osijek | Kovacevic V.,Josip Juraj Strossmayer University of Osijek
Turkish Journal of Agriculture and Forestry | Year: 2012

A field trial with the application of 3 rates (0, 5, and 20 t ha-1) of hydratized calcite (73% CaO + 2%-3% MgO + 21% H2O) was set up in the spring of 2006. The experiment area had 3 plots of 630 m2. Each plot was divided into 4 subplots for replications. Over the next 2 years, the residual effects of liming on plant yield and nutrient uptake were investigated using maize hybrid OsSK499 in 2006 and 2007 and soybean variety Ika in 2008. The ear leaves of maize at flowering (mid-July 2006 and 2007) were analyzed for P, K, Ca, Mg, S, Zn, Mn, Fe, Cu, Mo, and B concentrations in leaf dry matter. In general, liming was more effective in 2007 than in 2006, based on the significant influence on Mg (+34%), Ca (+10%), Mo (+24%), K (-13%), B (-20%), Mn (-68%), and Zn (-19%) concentrations in the leaves. Liming increased maize yield up to 33% and 35% in 2006 and 2007, respectively. In the third year of testing, soybean responded with a considerable yield increase of up to 44%. A very high soybean yield might be the result of the high yield capacity of the Ika cultivar and favorable weather conditions. However, based on leaf analysis, the nutritional status of soybean was unbalanced because of inadequate concentrations of K, Mg, and Mo. Additionally, P, Cu, and B concentrations were at the lowest limit of an adequate status. However, the leaf nutrient status of Ca, Zn, and Mn could be defined as adequate. © TÜBITAK.


Simek R.,Osijek Agricultural Institute | Novoselovic D.,Osijek Agricultural Institute
Poljoprivreda | Year: 2012

Mutations are origin of novel variabilities. With developing new methods there are new possibilities for plant breeding for desirable characteristics. The aim of this paper is to introduce a method of reverse genetics, particularly TILLING method, and its potential for future scientific work and creating new improved cultivars for both, quantitative and qualitative characteristics. It can be used for plant breeding and getting new information on molecular level. Mutations in TILLING method are induced with chemical reagent ethyl methanesulfonate - EMS. The function of EMS is to cause mismatch in DNA heteroduplex. TILLING can be used in a numerous plant species. EcoTILLING is the method used for studying natural populations. Both of these methods have advantage for not being trans-genetic, but labour and cost effective.


Hristov N.,Serbian Institute of Field and Vegetable Crops | Mladenov N.,Serbian Institute of Field and Vegetable Crops | Djuric V.,Serbian Institute of Field and Vegetable Crops | Kondic-Spika A.,Serbian Institute of Field and Vegetable Crops | And 2 more authors.
Euphytica | Year: 2010

The objective of this study was to determine genotype × environment interactions (GEI) obtained in wheat production in southeast Europe for protein content, sedimentation value, and loaf volume. Twenty wheat genotypes divided in two groups with different combinations of high molecular weight glutenin subunits (HMW-GS) were analyzed in 15 environments (E). In a combined analysis of variance, effects of genotype, environment, and their interaction (GEI).were highly significant for almost all theanalyzed traits. All the three traits were influenced more by location than by year, with protein content and sedimentation value being more responsive to changes in environmental conditions than loaf volume.The genotypes with high protein content had the regression coefficient value (b) close to 1, The genotypes with high protein content had the regression coefficient value (b) close to 1,. There were no significant differences in average values for the analyzed traits and adaptability parameters between the two groups of genotypes. It means that, concerning wheat quality improvement, genotypes with different HMW-GSs on the Glu-1D locus could be recommended for growing in southeast Europe. It is necessary to take into account the differences in adaptability of protein content to either positive or negative environmental changes that were observed between the groups. © 2009 Springer Science+Business Media B.V.


Zdunic Z.,Osijek Agricultural Institute | Grljusic S.,Osijek Agricultural Institute | Ledencan T.,Osijek Agricultural Institute | Duvnjak T.,Osijek Agricultural Institute | Simic D.,Osijek Agricultural Institute
Hereditas | Year: 2014

Characterizing concentrations of several beneficiary and toxic metals in maize leaves is of importance for ionomic studies and for silage production. The intermated B73 × Mo17 maize population (IBM) was evaluated for concentrations of eight metals (cadmium - Cd, copper - Cu, iron - Fe, potassium - K, magnesium - Mg, manganese - Mn, strontium - Sr and zinc - Zn) in ear-leaf to map quantitative trait loci (QTL) with 2161 molecular markers across the genome. QTL analysis revealed nine significant QTLs for concentrations of Cd, Cu, Fe, K, Mg and Sr combined over two environments. Median resolution for the QTL interval was less than 1 cM on a regular F2 map, which is a big improvement compared with the prior mapping (8 cM). The highest LOD scores of 15.52 and 15.31 were detected for K and Cd concentrations, respectively, explaining more than 20 percent of the phenotypic variance. No QTLs were found to be colocalized. QTL mapping in the IBM population did not confirm our earlier QTL results demonstrating considerable QTL ×genetic background interaction. The only exception is confirmation of the major QTL for Cd accumulation on chromosome 2. Our results could facilitate further genetic and physical mapping of genes for metal accumulation in maize. © 2014 The Authors.

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