Osaka Universicty

Ōsaka, Japan

Osaka Universicty

Ōsaka, Japan
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Rahayu R.,Osaka University | Ohsaki E.,Osaka University | Omori H.,Osaka Universicty | Ueda K.,Osaka University
Virology | Year: 2016

In latent infection of Kaposi's sarcoma-associated herpesvirus (KSHV), viral gene expression is extremely limited and copy numbers of viral genomes remain constant. Latency-associated nuclear antigen (LANA) is known to have a role in maintaining viral genome copy numbers in growing cells. Several studies have shown that LANA is localized in particular regions on mitotic chromosomes, such as centromeres/pericentromeres. We independently examined the distinct localization of LANA on mitotic chromosomes during mitosis, using super-resolution laser confocal microscopy and correlative fluorescence microscopy-electron microscopy (FM-EM) analyses. We found that the majority of LANA were not localized at particular regions such as telomeres/peritelomeres, centromeres/pericentromeres, and cohesion sites, but at the bodies of condensed chromosomes. Thus, LANA may undergo various interactions with the host factors on the condensed chromosomes in order to tether the viral genome to mitotic chromosomes and realize faithful viral genome segregation during cell division. © 2016.


Hara H.,Osaka Universicty | Takahashi T.,Osaka Universicty | Miyazaki Y.,Osaka Universicty | Kurokawa Y.,Osaka Universicty | And 6 more authors.
Japanese Journal of Cancer and Chemotherapy | Year: 2014

A male patient in his 40s presented with abdominal pain and mass palpitation. He was diagnosed with a submucosal gastric tumor and referred to our clinic. Computed topography (CT) scan revealed a large gastric tumor and multiple hepatic metastases He was diagnosed with unresectable gastrointestinal stromal tumor (GIST) and began imatinib treatment. However 2 weeks later the patient developed a high fever and abdominal pain. Tumor necrosis and abscess formation was observed on the CT scan We performed emergency surgery, which comprised partial gastrectomy, distal pancreatectomy, and splenectomy combined with radiofrequency ablation (RFA) for the hepatic metastases. Subsequently, he continued imatinib therapy However a new lesion developed in the liver and was diagnosed as imatinib-resistant disease. The new lesion was treated with RFA Eight years after the treatment, a new lesion occurred in the liver, which was difficult to excise owing to the location. Accordingly, a right-hepatic lobectomy was performed. Currently, he is alive and continues on imatinib therapy.


PubMed | Osaka Universicty
Type: Case Reports | Journal: Gan to kagaku ryoho. Cancer & chemotherapy | Year: 2015

A male patient in his 40s presented with abdominal pain and mass palpitation. He was diagnosed with a submucosal gastric tumor and referred to our clinic. Computed topography (CT) scan revealed a large gastric tumor and multiple hepatic metastases. He was diagnosed with unresectable gastrointestinal stromal tumor (GIST) and began imatinib treatment. However, 2 weeks later, the patient developed a high fever and abdominal pain. Tumor necrosis and abscess formation was observed on the CT scan. We performed emergency surgery, which comprised partial gastrectomy, distal pancreatectomy, and splenectomy, combined with radiofrequency ablation (RFA) for the hepatic metastases. Subsequently, he continued imatinib therapy. However, a new lesion developed in the liver and was diagnosed as imatinib-resistant disease. The new lesion was treated with RFA. Eight years after the treatment, a new lesion occurred in the liver, which was difficult to excise owing to the location. Accordingly, a right-hepatic lobectomy was performed. Currently, he is alive and continues on imatinib therapy.

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