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Nitta A.,Kagoshima National College of Technology | Aozasa M.,Osaka Butsuryo College
Journal of the Vacuum Society of Japan | Year: 2012

The effects of N 2 [mass number: 28] and O 2 [mass number: 32] gas impurities on ZnS host material and the emission center in the active layers of a thin-film electroluminescent device were examined for the impurity gas concentration. Electron spin resonance (ESR) experiments were performed to examine theMn 2+ cohesive state. As the partial pressure of N 2 increased, the L 30 and the maximum luminance eciency decreased, the threshold electric field strength increased due to the reduction in the concentration of the isolated single Mn 2+. When the partial pressure of N 2 was increased, the isolated single Mn 2+ and the clustered Mn 2+ concentration decreased and the interaction between the isolated singleMn 2+ and the clusteredMn 2+ increased.When O 2 gas impurity is introduced to the sputtering environment, some Zn atoms combine with O causing a deficiency of S. Copyright © 2002 - 2012 Hogrefe Publishing.


Kato S.,Prefectural University of Hiroshima | Kato S.,Osaka City University | Asada R.,Osaka Butsuryo College | Kageyama K.,Osaka Butsuryo College | And 2 more authors.
Cytotechnology | Year: 2011

The aim of the present study is to determine the anti-proliferative activity of 6-o-palmitoyl-l-ascorbic acid (Asc6Palm) that is a lipophilic derivative of l-ascorbic acid (Asc), on human tongue squamous carcinoma HSC-4 cells by combined use of hyperthermia in comparison to Asc. Asc6Palm or Asc were administered to HSC-4 cells for 1 h, to which hyperthermia at 42 °C was applied for initial 15 min. After further 1-72 h incubation at 37 °C, cell proliferation was determined with Crystal Violet staining. Ascorbyl radical (AscR) in HSC-4 cell suspension was measured by electron spin resonance (ESR), and cell morphology was observed with scanning electron microscopy (SEM). At 37 °C, 4 mM Asc or 0.35 mM Asc6Palm were enough to suppress proliferation of HSC-4 cells. By combined use of hyperthermia at 42 °C, cell proliferation was decreased when compared to 37 °C. After Asc of 4 mM was incubated with HSC-4 cell suspensions at 37 °C or 42 °C for 0-180 min, the signal intensity of ascorbyl radical (AscR) by ESR was not different regardless of the presence or absence of cells at 37 °C, whereas AscR signal was enlarged in the presence of HSC-4 cells at 42 °C. It was suggested that oxidation of Asc occurred rapidly in HSC-4 cells by hyperthermia, and thereby enhanced the anti-proliferative activity. By SEM observation, the surface of HSC-4 cells treated with Asc6Palm revealed distinct morphological changes. Thus, the combined regimen of Asc6Palm and hyperthermia is expected to exert a marked antitumor activity. © 2011 Springer Science+Business Media B.V.


Kato S.,Osaka City University | Kato S.,Japanese Center for Anti Aging MedSciences | Kimura M.,Osaka City University | Kageyama K.,Osaka Butsuryo College | And 2 more authors.
Journal of Nanoscience and Nanotechnology | Year: 2012

The nitroimidazole-related hypoxic radiosensitizer, pimonidazole (Pmz) was encapsulated in liposome composed of dipalmitoylphosphatidylcholine, cholesterol and dipalmitoylphosphatidylglycerol (molar ratio = 1:1:0.2; diameter = 1129 nm), and the radiosensitization was evaluated in human melanoma cells HMV-II. Cell proliferation was examined by WST-8 assay after X-ray irradiation in the presence of liposomal Pmz or free-Pmz under hypoxic conditions. On 7th day after X-ray irradiation of 5 Gy, cell proliferation decreased more markedly in the administration of liposomal Pmz than free-Pmz at equivalent Pmz doses. Chromatin fragmentation or nuclear condensation was observed in liposomal Pmz-treated HMV-II cells. Radiosensitization was enhanced dose-dependently along with Pmz amounts of 250-2000 μM contained in liposomal Pmz. Intracellular uptake was more abundant for liposomal Pmz for 60-240 min than for free-Pmz. Thus liposomal Pmz has a potential to overcome radiation resistance in hypoxia, owing to enhanced intracellular uptake by melanoma cells. Copyright © 2012 American Scientific Publishers All rights reserved.


Asada R.,Osaka Butsuryo College | Kageyama K.,Osaka Butsuryo College | Kimura M.,Osaka City University | Saitoh Y.,Prefectural University of Hiroshima | Miwa N.,Prefectural University of Hiroshima
Oncology Letters | Year: 2012

In this study, using human tongue squamous carcinoma cells (HSC-4) carcinostatic activity was compared for diverse L-ascorbic acid (Asc) derivatives, including the 'straight-C 16-chain types', 6-O-palmitoyl-Asc (A6-P) and Asc-2-phosphate-6-O-palmitate sodium salt (APPS), as well as the 'branched-C 16-chain types', Asc-2-phosphate-6-O-(2'-hexyl)decanoate (APHD), an isomer of APPS, and Asc-2,3,5,6-O-tetra-(2'-hexyl)decanoate (VCIP). The order of magnitude of the carcinostatic effects at 37 °C was: APPS>A6-P = APHD>VCIP and at 42 °C was APPS = A6-P>APHD>VCIP. Therefore, the two straight-C 16-chain derivatives, APPS and A6-P, had a greater effect compared to the two branched-C 16-chain Asc derivatives, which are considered to have more difficulty with 'orientation along cell-membrane-glycerolipid direction'. APPS-treated HCS-4 cells were observed for a decrease in cell number, cell shrinkage, pycnosis indicative of apoptosis and cell deformation. The order of cytotoxicity for the normal human dermal fibroblasts (OUMS-36) at 37 °C was: A6-P (50% inhibitory concentration: 150-300 μM)>APHD (450-600 μM)>>Asc = APPS (800-1000 μM). Accordingly, APHD was more cytotoxic than APPS, since the straight-C 16-chain type, which was eliminated after the enzymatic esterolysis of APPS, is metabolized via the 'fatty acid β-oxidation cycle' more efficiently in normal cells. Thus, APPS had a greater advantage over APHD, A6-P and VCIP in terms of carcinostatic effects at 37 °C, carcinostasis promotion at 42 °C and a decrease of cytotoxicity to normal cells. This observation suggests a marked potential for aliphatic chain-moiety structures as anticancer agents, due to their cancer-selective carcinostasis and combined efficacy with hyperthermia, without causing side effects.


Fujiwara Y.,University of Fukui | Fujiwara Y.,Kanazawa University | Kimura H.,University of Fukui | Miyati T.,Kanazawa University | And 5 more authors.
Magnetic Resonance Materials in Physics, Biology and Medicine | Year: 2012

Object To propose a new arterial spin labeling (ASL) perfusion-imaging method (alternate slab width inversion recovery ASL: AIRASL) that takes advantage of the qualities of 3.0 T. Materials and methods AIRASL utilizes alternate slab width IR pulses for labeling blood to obtain a higher signalto-noise ratio (SNR). Numerical simulations were used to evaluate perfusion signals. In vivo studies were performed to show the feasibility of AIRASL on five healthy subjects. We performed a statistical analysis of the differences in perfusion SNR measurements between flow-sensitive alternating inversion recovery (FAIR) and AIRASL. Results In signal simulation, the signal obtained by AIRASL at 3.0 and 1.5 T was 1.14 and 0.85%, respectively, whereas the signal obtained by FAIR at 3.0 and 1.5 T was 0.57 and 0.47%, respectively. In an in vivo study, the SNR of FAIR (3.0 T) and FAIR (1.5 T) were 1.73 ± 0.49 and 1.02 ± 0.20, respectively, whereas the SNRs of AIRASL (3.0 T) and AIRASL (1.5 T) were 3.93 ± 1.65 and 1.34 ± 0.31, respectively. SNR in AIRASL at 3.0 T was significantly greater than that in FAIR at 3.0 T. Conclusion The most significant potential advantage of AIRASL is its high SNR, which takes advantage of the qualities of 3.0 T. This sequence can be easily applied in the clinical setting and will enable ASL to become more relevant for clinical application. © ESMRMB 2012.


Asada R.,Osaka Butsuryo College | Kageyama K.,Osaka Butsuryo College | Tanaka H.,Osaka Butsuryo College | Matsui H.,Hiroshima Kasei Ltd. | And 3 more authors.
Oncology Reports | Year: 2010

In order to erase reactive oxygen species (ROS) related with the proliferation of tumor cells by reducing activity of hydrogen, we developed functional water containing nano-bubbles (diameters: <900 nm for 71%/ population) hydrogen of 1.1-1.5 ppm (the theoretical maximum: 1.6 ppm) with a reducing ability (an oxidation-reduction potential -650 mV, normal water: +100-200 mV) using a microporous-filter hydrogen-jetting device. We showed that hydrogen water erased ROS indispensable for tumor cell growth by ESR/spin trap, the redox indicator CDCFH-DA assay, and was cytotoxic to Ehrlich ascites tumor cells as assessed by WST-8 assay, crystal violet dye stain and scanning electron microscopy, after 24-h or 48-h incubation sequent to warming at 37°C or 42°C. Hydrogen water supplemented with platinum colloid (0.3 ppm Pt in 4% polyvinylpyrrolidone) had more antitumor activity than hydrogen water alone, mineral water alone (15.6%), hydrogen water plus mineral water, or platinum colloid alone as observed by decreased cell numbers, cell shrinkage and pycnosis (nuclear condensation)/karyorrhexis (nuclear fragmentation) indicative of apoptosis, together with cell deformation and disappearance of microvilli on the membrane surface. These antitumor effects were promoted by combination with hyperthermia at 42°C. Thus, the nano-bubble hydrogen water with platinum colloid is potent as an antitumor agent.


Asada R.,Osaka Butsuryo College | Kageyama K.,Osaka Butsuryo College | Tanaka H.,Osaka Butsuryo College | Saitoh Y.,Prefectural University of Hiroshima | Miwa N.,Prefectural University of Hiroshima
Molecular Medicine Reports | Year: 2012

The antitumor and anti-invasive activities of the low-molecular-weight macrocyclic ketones (MCKs), such as musk secreted from the mammalian genital glands and musk released from relatively unkown plants, were investigated comparatively together with the enhancement of the effects in combination with hyperthermia. Ehrlich ascites tumor cells were treated with each MCK and cultured, followed by evaluation of the cell viability using the mitochondrial dehydrogenase-based WST-8 assay. The number of HT-1080 human fibrosarcoma cells cultured with the MCKs or invading through a reconstituted basement membrane was measured using microscopy. The order of the efficiency was as follows: (Z)-g-cycloheptadecen-1-one (Hp) (17:1, musk rats), 8-cyclohexadecen-1-one (16:1, musk ferns), cyclopentadecanone (15:0, musk rats) and 3-methylcyclopentadecanone (16:0, musk deer), having 15-17 carbon atoms with and without a double bond, which exhibited a carcinostatic effect either at 100 μM for 20-h culture or at 50 μM for 72-h culture. The effects were markedly enhanced by heat treatment at 42°C. MCKs were not found in the cells by gas-liquid chromatographic determination, indicating that the carcinostatic effects were attributed to their surface activity on the cell membrane. Invasion of HT-1080 cells was inhibited by MCKs at doses scarcely diminishing the cell viability, indicating that the suppression of invasiveness did not ensue from the secondary action due to carcinostasis. The order of invasion-inhibitory efficacy of the MCKs was, however, similar to that of their carcinostatic effects. Hp17:1 also exhibited the highest anti-invasive activity in addition to the highest carcinostatic activity. The two inhibitory effects were promoted by combination with hyperthermia. MCKs with a double bond, particularly Hp17:1 rather than 8-Hx16:1, but not saturated-aliphatic MCKs, may be potent multi-applicable antitumor agents due to their dual inhibitory activities against tumor progression and invasion and in hyperthermia-combined therapy.


Nishiura M.,Osaka University | Nishiura M.,Osaka Butsuryo college | Yasuhiro T.,Osaka University | Murase K.,Osaka University
Magnetic Resonance Imaging | Year: 2011

Purpose: The aim of this study was to retrospectively evaluate the ability of dynamic, contrast-enhanced magnetic resonance imaging (DCE-MRI) to differentiate between ductal carcinoma in situ (DCIS) and mastopathy by analyzing their signal intensities (SIs). Methods: After the pre-contrast MRI was performed using a 1.5-T MRI system, DCE-MRI was performed four times following intravenous administration of contrast medium. We set the volumes of interest (VOIs) on the tumor and normal mammary gland and obtained the SIs in these VOIs. We calculated the entropy (EPY) in the pre-contrast (EPY0) and four post-contrast scans (EPY1, EPY2, EPY3, and EPY4 for the first, second, third and fourth scans, respectively) using the volume histogram method, and the wash-in (WRin) and washout rates (WRout) according to the Breast-Imaging Reporting and Data System developed by the American College of Radiology. We also calculated the early slope (Slopeearly) from the pre- and post-contrast SIs in the tumor and normal gland. We evaluated the usefulness of the above parameters for differentiating between DCIS and mastopathy using the area under the receiver operating characteristic curve (Az). Results: There were significant differences in EPY2 (P=009), EPY3 (P=017), EPY4 (P=034), WRin (P=036), WRout (P=019), and Slopeearly (P=002) between DCIS and mastopathy. The average Az values were 0.67, 0.52, 0.64, 0.63, 0.67 and 0.70 for EPY2, EPY3, EPY4, WRin, WRout and Slopeearly, respectively. Conclusion: We evaluated the usefulness of various parameters calculated from SIs obtained by DCE-MRI for differentiating between DCIS and mastopathy. Our results suggested that Slopeearly is more useful than EPYs, WRin and WRout. © 2011 Elsevier Inc.


Nishiura M.,Osaka University | Nishiura M.,Osaka Butsuryo College | Tamaki Y.,Osaka University | Murase K.,Osaka University
European Journal of Radiology | Year: 2011

The purpose of this study was to retrospectively evaluate the feasibility of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) to differentiate between ductal carcinoma in situ (DCIS) and mastopathy by analyzing their time-intensity curves (TICs) using the two-compartment pharmacokinetic model with an assumption of instantaneous injection of contrast medium (TCPM). After the pre-contrast MRI was performed using a 1.5 T MRI system, DCE-MRI was performed four times after the intravenous administration of contrast medium. We set the volumes of interest (VOIs) on the tumor and normal mammary gland, and obtained the TICs in these VOIs. We calculated the following parameters by fitting these TICs to the equation derived from TCPM; the initial slope of the TIC (Slope ini), the area under the TIC (AUC), the time to peak enhancement (TTP) and the peak enhancement (PeakE). We calculated these parameters in both the lesion and normal mammary gland and the ratios of the parameters in the lesion to those in the normal gland (rSlope ini, rAUC, rTTP and rPeakE). There were significant differences in Slope ini (P = 0.009), PeakE (P = 0.019), rSlope ini (P = 0.010), and rTTP (P = 0.005) between DCIS and mastopathy. The areas under the receiver operating characteristic curve for Slope ini, PeakE, rSlope ini, and rTTP were 0.67 ± 0.06 (P = 0.009), 0.65 ± 0.06 (P = 0.019), 0.67 ± 0.06 (P = 0.01), and 0.68 ± 0.06 (P = 0.005), respectively. In conclusion, our results suggest that analysis of TICs obtained by DCE-MRI using TCPM appears to be useful for differentiating between DCIS and mastopathy. © 2010 Elsevier Ireland Ltd.


Takai I.,Osaka Butsuryo College
Japanese Journal of Geriatrics | Year: 2010

Aim: Recent research suggests that the fear of falling may be a more serious problem than actual falling among frail elderly people. The purpose of this study was to investigate whether or not it is possible to influence the fear of falling among frail elderly people who previously had fallen, by balance exercises under attention tasks. Methods: The subjects were 22 frail elderly people in nursing homes (3 men and 19 women, mean age 83.1±5.2 SD)who had fallen. Written informed consent was given for participation in the study. Subjects were divided into two groups at random: the balance exercise group (the control group) and the balance exercise during an attention task (the attention group). Balance exercise continued for 10 weeks, consisting of one 5 minute session, 3 times weekly. The control and intervention subjects were evaluated using the Fall Efficacy Scale (FES), Functional Reach Test (FRT),Timed Up & Go Test (TUGT) and walking speed, before the program, at the end of the trial, and 10 weeks after the program. Results: There was no change in walking speed in either group, and difference in TUGT of the control group was observed. We found improvement in FRT and TUGT of the attention group. The FES decreased in the attention group. Conclusion: These findings indicate that balance exercise during an attention task can improve standing posture balance in frail elderly people who have fallen. These results also suggest that it is important to intervene in both motor function and attention function to decrease the fear of falling.

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