Cai T.-Y.,Orthopedics Hospital |
Chen X.-S.,Shanghai University |
Jia L.-S.,Shanghai University |
Sun Y.-Q.,Orthopedics Hospital |
And 2 more authors.
Chinese Journal of Tissue Engineering Research | Year: 2014
Background: Exogenous basic fibroblast growth factor (bFGF) plays an important role in the ligament tissue healing process, and the use of transgenic methods to transfect exogenous genes into cells can promote the secretion of bFGF. Objective: To observe phenotypic changes and the bFGF protein expression after bFGF recombinant adenovirus was used to transfect rabbit bone marrow mesenchymal stem cells (BMSCs). Methods: Passage 2 BMSCs were divided into three groups: Ad. bFGF-eGFP group, Ad. eGFP group and control group. Under a phase contrast microscope we observed the changes in cell morphology. The expression of bFGF protein in BMSCs was determined by enzyme-linked immunosorbent assay (ELISA). The proliferative curve was detected by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Results And Conclusion: The transfected cells showed a uniform phenotype of fibroblasts. MTT colorimetric assay revealed that more proliferative activity of transfected BMSCs was shown in the Ad. bFGF-eGFP group than in the Ad. eGFP group and control group. ELISA results showed that expression of bFGF protein was higher in the Ad. bFGF-eGFP group than in the Ad. eGFP group and control group (P < 0. 05). BFGF recombinant adenovirus can induce the differentiation of BMSCs into fibroblasts, increase proliferative ability and promote the expression of bFGF protein.
Lu Z.-D.,Orthopedics Hospital |
Zhai M.-Y.,Orthopedics Hospital |
Yang Y.,Orthopedics Hospital |
Liu H.-Y.,Peking University
Journal of Clinical Rehabilitative Tissue Engineering Research | Year: 2010
BACKGROUND: Anterior cervical discectomy and fusion has been considered the gold standard for treatment of symptomatic degenerative disc disease within the cervical spine. However, fusion may result in progressive degeneration of the adjacent segments. Artificial disc replacement seems to be promising, segmental motion and stability are preserved while the spinal canal has been enlarged, and the intervertebral disc biological mechanics has been preserved, but not leads to progressive degeneration of the adjacent segments. OBJECTIVE: To observe the functional outcome and kinematics after the Prodisc artificial neck intervertebral disc replacement. METHODS: A total of 55 cases (69 intervertebral discs), 29 males and 26 females, aged 48 (31-76) years were selected, including 14 with bi-segmental injury. There were 32 cases of cervical spondylotic myelopathy, 9 of cervical spondylotic radiculopathy, and 14 of mixed type cervical spondylosis. Prodisc artificial neck intervertebral disc replacement was performed using anterior or oblique incision. Prospective data JOA score and kinematic measures were collected before surgery and at 3, 6, 12, and 24 months after surgery. Range of motion was determined by independent radiologic assessment of flexion-extension radiographs. RESULTS AND CONCLUSION: The 55 patients were followed-up, including 41 undergoing single segmental disc replacement and 14 undergoing bi-segmental disc replacement. They were followed up for 22 (56-48) months postoperatively. JOA scores displayed improved cervical function by 56%. Range of motion of sagittal and coronal planes were similar to those prior to operation compared with preoperatively (P = 0.45, 0.74), and the range of motion and stability were maintained as adjacent segments. Results showed that the artificial disc replacement maintained range of motion of original intervertebral disc, accelerated patient recovery, and slightly affected adjacent intervertebral disc.