Orenburg State Medical Academy

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Orenburg, Russia
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Senchukova M.,Orenburg State Medical Academy | Kiselevsky M.V.,Institute of Experimental Diagnostics and Therapy of Tumors
Journal of Cancer | Year: 2014

The "cavitary" type of angiogenesis in patients with gastric cancer (GC) is described for the first time. Material and methods: The samples of tumour and adjacent gastric mucosa (GM) in 73 patients with GC who had undergone radical surgery were being studied. The sections were stained with hematoxylin and eosin (H and E) and immunohistochemically (IGH) using antibodies to CD34. Results: ? new type of vessel formation consists of the appearance of cavitary structures (CS) in tumours and the adjacent GM, which are then lined by endothelial cells and merged into the blood vessels of the organ. We believe that the CS can be formed by means: 1) of the abruption of layers of epithelial cells (both normal and tumoral) from their underlying foundation and their desquamation into the lumen of the "obliterated" gastric glands (GG); 2) of the dilatation of the GG and thinning of their walls; 3) of the formation of "cavity" directly in the lamina propria of GM or in the tumoral stroma. It was noted that only the presence of multiple "cavitary" vessels (CV) of type-1 had been associated with the decrease of 3-year overall survival (OR=15,0, 95%CI=2,96-76,31) and relapse-free survival (OR=14,93, 95%CI=4,34-51,38). We also observed the improvement of the long-term outcomes in patients with GC having received antibacterial therapy (AT) before surgery that can be associated with its influence on the formation of CV type-1. Conclusion: The described new type of angiogenesis is of great clinical importance. © Ivyspring International Publisher.


Nikonorov A.A.,Orenburg State Medical Academy | Skalnaya M.G.,Russian Society of Trace Elements in Medicine | Tinkov A.A.,Orenburg State Medical Academy | Skalny A.V.,Russian Society of Trace Elements in Medicine
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS) | Year: 2015

Obesity is identified as an important medical problem. One of the pathologic conditions observed in obesity is systemic iron deficiency and hypoferremia. Along with a large number of studies indicating disturbed iron homeostasis in obesity, recent data indicate a cause-effect relationship between iron status and obesity-related pathologies. The primary objective of the article is to consider two aspects of the iron-obesity interplay: (1) the mechanisms leading to impaired iron balance, and (2) the pathways of iron participation in obesity-related pathogenesis. While considering disturbance of iron homeostasis in obesity, a number of potential mechanisms of hypoferremia are proposed. At the same time, the inflammation of obesity and obesity-related hepcidin and lipocalin 2 hyperproduction seem to be the most probable reasons of obesity-related hypoferremia. Oversecretion of these proteins leads to iron sequestration in reticuloendothelial system cells. The latter also leads to increased adipose tissue iron content, thus producing preconditions for adverse effects of local iron overload. Being a redox-active metal, iron is capable of inducing oxidative stress as well as endoplasmic reticulum stress, inflammation and adipose tissue endocrine dysfunction. Iron-mediated mechanisms of toxicity may influence aspects of obesity pathogenesis possibly even leading to obesity aggravation. Thus, a mutual interaction between disturbance in iron homeostasis and obesity pathogenesis is proposed. All sides of this interaction should be considered to design new therapeutic approaches to the treatment of disturbed iron homeostasis in obesity. Copyright © 2014 Elsevier GmbH. All rights reserved.


Nikonorov A.A.,Orenburg State Medical Academy | Skalnaya M.G.,RUSn Society of Trace Elements in Medicine | Tinkov A.A.,Orenburg State Medical Academy | Skalny A.V.,Orenburg State University
Journal of Trace Elements in Medicine and Biology | Year: 2015

Obesity is identified as an important medical problem. One of the pathologic conditions observed in obesity is systemic iron deficiency and hypoferremia. Along with a large number of studies indicating disturbed iron homeostasis in obesity, recent data indicate a cause-effect relationship between iron status and obesity-related pathologies. The primary objective of the article is to consider two aspects of the iron-obesity interplay: (1) the mechanisms leading to impaired iron balance, and (2) the pathways of iron participation in obesity-related pathogenesis. While considering disturbance of iron homeostasis in obesity, a number of potential mechanisms of hypoferremia are proposed. At the same time, the inflammation of obesity and obesity-related hepcidin and lipocalin 2 hyperproduction seem to be the most probable reasons of obesity-related hypoferremia. Oversecretion of these proteins leads to iron sequestration in reticuloendothelial system cells. The latter also leads to increased adipose tissue iron content, thus producing preconditions for adverse effects of local iron overload. Being a redox-active metal, iron is capable of inducing oxidative stress as well as endoplasmic reticulum stress, inflammation and adipose tissue endocrine dysfunction. Iron-mediated mechanisms of toxicity may influence aspects of obesity pathogenesis possibly even leading to obesity aggravation. Thus, a mutual interaction between disturbance in iron homeostasis and obesity pathogenesis is proposed. All sides of this interaction should be considered to design new therapeutic approaches to the treatment of disturbed iron homeostasis in obesity. © 2014 Elsevier GmbH.


Malyshkin A.P.,Orenburg State Medical Academy
Immunobiology | Year: 2010

There is no fundamental difference in contagiousness or pathogenicity between the normal and pathogenic microfloras: both are contagious, and both may either cause illness or persist in the body of a healthy carrier. The contagiousness of the normal microflora is determined by its positive function in the host. In addition to the normal microflora, endogenous retrovirus genes are also useful for a macroorganism: they have been found to be integral elements of many animal and plant genomes and participate in vital functions, such as genome activity control, antiviral defense, and formation of the placenta; dysfunction of some endogenous retrovirus genes causes pathology. Components of bacterial cells cause protective effects with respect to noninfectious somatic diseases. Therefore, it is reasonable to assume that natural susceptibility to both "normal" and "pathogenic" microfloras results from vital necessity for a macroorganism to interact with them. The necessity to interact with specific microorganisms and, hence, susceptibility to them is the cause of their infectivity. In this connection, studies on the positive functions of specific "pathogenic" microflora in a macroorganism with the use of SPF animals would be of interest. Even more interesting would be experiments with transfer of specific microbial genes into the genomes of susceptible species, which may help to understand the causes and mechanisms of susceptibility/insusceptibility as a basis of species immunity and develop fundamentally new and more natural methods for preventing and treating infectious diseases. © 2009 Elsevier GmbH.


Tinkov A.A.,Orenburg State Medical Academy | Polyakova V.S.,Orenburg State Medical Academy | Nikonorov A.A.,Orenburg State Medical Academy
BioMetals | Year: 2013

The primary objective of this research project is explore a possible adipogenic effect of iron and/or copper in albino Wistar rats kept on standard (STD) and high-fat (HFD) diets. The female Wistar rats in the study were divided into eight experimental groups (n = 6). Rats maintained on STD and HFD received 3 mg/l FeSO4·7H2O, 4.88 mg/l CuSO4 and a combination of 1.5 mg/l FeSO4·7H2O and 2.44 mg/l CuSO4 with drinking water. Control groups were kept on STD and HFD and received pure water without metal salts. Consumption of iron and copper in the groups of rats maintained on an STD did not produce a significant increase in weight, adipose tissue content or body mass index. However, the adipocyte size and infiltration were increased in the adipose tissue of STD-fed rats receiving a mixture of iron and copper with drinking water. The rats fed iron and copper and, especially, their combination on a HFD background had a significantly higher weight gain, adipose tissue content, morphometric parameters values and adipocyte size compared to STD- and HFD-fed controls. Iron and copper consumption produced their accumulation in the rats' adipose tissue. Moreover, the studied metals reduced adipose tissue concentration of chromium and vanadium. The lipoprotein profile and serum oxidative stress biomarkers were affected in the rats receiving the metals and STD. Hyperglycemia was observed in the rats receiving the studied metals on HFD-background. Based on the analysis of the test subjects, the study suggests that iron and copper administration, especially combined, may potentiate adipogenic effect of HFD. © 2013 Springer Science+Business Media New York.


Aptikeeva N.V.,Orenburg State Medical Academy
Zhurnal Nevrologii i Psihiatrii imeni S.S. Korsakova | Year: 2015

This literature review is devoted to the differential diagnosis of central and peripheral vestibular vertigo from the point of view of modern otoneurology. In addition, an own case is given. © 2015, Media Sphera. All rights reserved.


Sizova L.,Orenburg State Medical Academy
Human Immunology | Year: 2012

The aim of this study was to evaluate the usefulness of antibodies to modified citrullinated vimentin (anti-MCV) for diagnosing early rheumatoid arthritis (RA) and to examine the correlations between anti-MCV and clinical parameters as well as radiographic and ultrasound data. Our results suggest that anti-MCV has a sensitivity and specificity of 53.3 and 83.3%, respectively, and a positive prognostic value of 96% in patients with early RA. Anti-MCV antibodies were observed in 48.5% of rheumatoid factor (RF)-negative RA patients in data from a latex test and in 35.7% of patients with negative results for immunoglobulin (Ig)-M-RF. The positive result for both anti-MCV and IgM-RF has a sensitivity of 42.2% and a specificity of 100%. No significant correlation was observed between anti-MCV and disease activity score using 28 joint counts, radiographic RA stage, number of erosions on ultrasonography of joints, and quality-of-life scores at disease onset. The Spearman correlation was significant in early RA patients with positive results of anti-MCV between the titers of this marker and arthritis duration, level of erythrocyte sedimentation rate, and IgM-RF. The best diagnostic strategy in early RA may be to assay both anti-MCV and IgM-RF. Thus, a question arises regarding the possible inclusion of anti-MCV in future revisions of the classification criteria of RA. © 2012 American Society for Histocompatibility and Immunogenetics.


Zheleznov L.M.,Orenburg State Medical Academy
Morfologii{combining double inverted breve}a (Saint Petersburg, Russia) | Year: 2012

Lung holotopy, skeletotopy and syntopy were studied in 70 human fetuses at developmental weeks 16-24 with N. I. Pirogov method, macro-microscopical preparation and using histotopographical sections in three imutually perpendicular planes. It was found that during weeks 16-18, the apex of the left lung was located posteriorly at the level of I intercostal space, at weeks 22-24--at the level of lower surface of I rib. At the right side, the apex was located at the level of upper surface of I rib during the whole period. The lower margin of the right lung was located at the level of IV rib during the whole period, while that of the left lung was detected at the level of III rib only during the beginning of the period. In the early fetal period, the projection of the root of the right lung extended from the lower margin of T(III) vertebral body toT(VI), while that one of the left lung was located at the level of the upper margins of T(IV)-T(VII) vertebral bodies. In the late period, these projections were found at the level of T(IV) (upper vertebral margin)--T(VII) (lower vertebral margin), and T(IV) (lower vertebral margin)--T(VIII) (upper vertebral margin) respectively. Intraorgan bronchi and pulmonary vessels were most clearly visualized in horizontal sections at T(III) -T(IX) levels. The results obtained should be taken into account when carrying out of diagnostic ultrasound and magnetic resonance studies of the fetus and surgical interventions on fetuses.


Objectives: In this study we investigated the diagnostic and prognostic significance of the 2002 European League Against Rheumatism (EULAR) criteria for the clinical suspicion of rheumatoid arthritis (RA) and the sensitivity of the new 2010 American College of Rheumatology (ACR)/EULAR classification criteria for RA in patients with "early arthritis". Patients and methods: The subjects were 250 outpatients (211 females, 39 males; mean age 46.1±11.8 years; range 19 to 73 years) with"early ar thritis". The mean disease duration was 4.9±3.3 months. The criteria of inclusion of patients in the study were: (i) patients aged 18 years and older, (ii) clinical suspicion of RA according to EULAR (2002) criteria or persistent arthritis/arthralgia (>6 weeks), (iii) duration of disease <1 year, and (iv) written consent of the patient to participate in the study. The exclusion criteria were: (i) presence of a definite diagnosis of other rheumatic diseases before the onset of the study, and (ii) refusal of the patient to participate in the study. The diagnostic significance of the criteria was determined by the sensitivity and specificity. Predictive significance was estimated on positive and negative predictive values. The diagnostic effectiveness was also considered. Results: The 2002 EULAR criteria had higher diagnostic accuracy in identifying all three criteria in the patients. The negative predictive values ranging from 87.84% to 98.0% indicate that the absence of one of these criteria, especially arthritis of three or more joints, reduces the probability of RA diagnosis and requires investigation for other possible diseases with involvement of the joints. Two criteria was found to have the highest sensitivity among the 2010 ACR/EULAR classification criteria for RA: (i) the presence of synovitis in at least one joint, and (ii) symptom duration ≥6 weeks."Definite" RA could be identified in 98 (86%) patients according to new criteria (a score of ≥6/10) at the beginning of the study. Conclusion: The obtained data indicates the feasibility of using the 2002 EULAR criteria for screening patients with early RA and the usefulness of the new 2010 ACR/EULAR classification criteria for identification patients with"definite RA" and initiation of therapy with disease-modifying antirheumatic drugs. © 2011 Turkish League Against Rheumatism. All rights reserved.


Sizova L.V.,Orenburg State Medical Academy
Indian Journal of Pharmacology | Year: 2011

The main aim of treatment of early rheumatoid arthritis (RA) should be to achieve clinical remission to prevent structural damage and physical disability. Arthrofoon modifies production/activity of endogenous inhibitors of tumor necrosis factor-(TNF-). The sublingual rout is the most acceptable to ambulatory treatment because it does not produce the adverse reactions associated with intravenous therapy. The treatment with arthrofoon in outpatient with early RA is analyzed here. This report is devoted to the 28-year-old Russian woman who received arthrofoon due to suspicion of early RA. The strategy of early prescription of ultra-low doses of TNF-antibody within two years was confirmed by the clinical improvement and delay of radiological disease progression in patient with undifferentiated arthritis or probable RA initially.

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