Orenburg State Agrarian University

Orenburg, Russia
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Sizova E.A.,Orenburg State University | Korolev V.L.,Federal Agency of Scientific Organizations | Makaev Sh.A.,Federal Agency of Scientific Organizations | Miroshnikova E.P.,Orenburg State University | Shakhov V.A.,Orenburg State Agrarian University
Sel'skokhozyaistvennaya Biologiya | Year: 2016

Diets of modern crosses and breeds of farm animals require mandatory correction of mineral nutrition. Ionic forms commonly used for correction are characterized by low bioavailability, prooxidant effect, and high toxicity in vivo. That is why low-toxic sources of essential chemical elements are of particular interest, including nanocrystalline metals. We firstly assessed the efficient of nanosized copper on the model of broiler chickens. A comparative study of productive and biological effects of copper nanoparticles (CuNPs) and copper sulfate was carried out on Smena 7 chickens of 14-42 days of age using different administration methods. Hematologic and biochemical parameters and elemental composition of tissues were assessed. We revealed an increase in red blood cells and hemoglobin and decrease of platelets at the highest doses of Cu NPs administrated orally or intramuscularly. The biochemical blood parameters assessed indicated an enhancement of plastic processes in the body and, as a consequence, an increase in the growth rate under Cu NPs administration. The difference of the total protein compared to the control (33.6 %, p < 0.05) was the highest when Cu NPs injected at a dose of 2 mg/kg which increased the supply of protein synthesis. The albumin level was higher in 28-day old chicks injected with Cu NPs (2 and 0.2 mg/kg). A replacement of dietary CuSO4 with dietary Cu NPs led to an increase in the growth rate. When Cu NPs fed, the found biochemical parameters indicated strengthening of energy and synthetic processes in the body, and therefore, an increase in the poultry growth. At 1.7 mg/kg of dietary Cu NPs the gained weight was 13.5-23.8 % (p < 0.01 to p < 0.001) more as compared to the control. Dietary Cu NPs at 0.7 mg/kg was not enough to highly stimulate the poultry growth and resulted in the weight gain which was lower than in the poultry fed with Cu NPs at 1.7 mg/kg but higher (by 8.5-18.4 %, p < 0.01 to p < 0.01) as compared to the poultry not fed with Cu NPs. Thus, the promoting effect depends on the form of copper source. The Cu NPs effectiveness under enteral administration at 1.7 mg/kg or intramuscular double injections at 0.2 mg/kg was superior to that of dietary copper sulfate.

Glinushkin A.P.,Federal Agency of Scientific Organization | Beloshapkina O.O.,Russian State Agricultural University | Solovykh A.A.,Orenburg State Agrarian University | Sudarenkov G.V.,Orenburg State Agrarian University | Molnar J.,Federal Agency of Scientific Organization
Acta Phytopathologica et Entomologica Hungarica | Year: 2016

The paper presents the results of a long-term monitoring of bacterial diseases of wheat in the steppe zone of the Southern Ural. A clear domination of the genus Xanthomonas over the genus Pseudomonas has been revealed (60 and 11% of the total samples collected, respectively). The frequency of a winter wheat infection with these two pathogens exceeded that of the spring wheat. The field phytosanitary assessment resulted in the observation of the earlier unknown manifestation of the Xanthomonas infection called as tip bacteriosis. The frequency of wheat infection with Xanthomonas sp. almost did not depend on the relief type, observation period, and sowing date, though the frequency of infection observed for the northern slope and lowland clearly exceeded those observed for other relief types. The frequency of infection with Pseudomonas sp. clearly depended on the sowing date and local relief type reaching the maximum at the lowlands (19-27%), so this relief type can be considered as a "signal" point for the early diagnostics of this pathogen. © 2016 Akadémiai Kiadó, Budapest.

Tinkov A.A.,Orenburg State Medical Academy | Ajsuvakova O.P.,Orenburg State Agrarian University | Skalnaya M.G.,Center for Biotic Medicine | Popova E.V.,Orenburg State Medical Academy | And 5 more authors.
BioMetals | Year: 2015

A significant interrelation between heavy metal exposure and metabolic syndrome (MetS) development has been demonstrated earlier. Despite the presence of a number of works aimed at the investigation of the role of Hg in MetS development, the existing data remain contradictory. Therefore, the primary objective of the current work is to review the existing data regarding the influence of mercury on universal mechanisms involved in the pathogenesis of the development of MetS and its components. The brief chemical characterization of mercury is provided. The role of mercury in induction of oxidative stress has been discussed. In particular, Hg-induced oxidative stress may occur due to both prooxidant action of the metal and decrease in antioxidant enzymes. Despite the absence of direct indications, it can be proposed that mercury may induce endoplasmic reticulum stress. As it is seen from both in vivo and in vitro studies, mercury is capable of inducing inflammation. The reviewed data demonstrate that mercury affects universal pathogenetic mechanisms of MetS development. Moreover, multiple investigations have indicated the role of mercury in pathogenesis of MetS components: dyslipidemia, hypertension, insulin resistance, and obesity to a lesser extent. The present state of data regarding the interrelation between mercury and MetS denotes the following perspectives: (1) Further clinic-epidemiologic and experimental studies are required to estimate the association between mercury exposure and the development of MetS components, especially obesity; (2) Additional investigations of the possible effect of organism's mercury content modulation on MetS pathogenesis should be undertaken. © 2015 Springer Science+Business Media New York.

Ajsuvakova O.P.,Orenburg State Agrarian University | Bezryadin S.G.,Orenburg State Agrarian University | Chevela V.V.,RAS Institute of Chemistry | Ivanova V.Y.,RAS Institute of Chemistry | Kuzyakin D.V.,Orenburg State Agrarian University
Oriental Journal of Chemistry | Year: 2014

The aqueous interactions between Ti(IV), Dy(III) and citric (H4Cit), d-tartaric (d-H4Tart) and racemic tartaric acids (dl-H4Tart) acids were investigated by potentiometric titration and nuclear magnetic relaxation methods. The analysis of experimental data was performed using mathematical models to evaluate the possibility of existence of a wide species' set in the solution and to identify those of which account is sufficient to reproduce the observed pattern. The species of different nuclearity were found, the relaxation efficiency (R1) and equilibrium constants (Kpmqr) of heteronuclear complexes were calculated. It is shown that the complexation processes are strongly pH-dependent. © 2014, Oriental Scientific Publishing Company. All rights reserved.

Nemereshina O.N.,Orenburg State University | Gusev N.F.,Orenburg State Agrarian University
Russian Journal of Biopharmaceuticals | Year: 2015

The anatomical and morphological study of Plantago maxima Juss. ex Jacq. Revealed the diagnostic features of the leaf blade, stem and root of the plant. The results obtained can be used in studies on the taxonomy of the genus Plantago L. and to diagnose a promising new type of medicinal plants — most of plantain (Plantago maxima Juss. ex Jacq.). A significant number of receptacles mucous substances in all studied organs of the plant, which partly explains the increased stability of the species to abiotic stress conditions in the steppe zone of the Urals are presented. © 2015. All right Reserved.

Vasilchenko A.S.,Orenburg State University | Dymova V.V.,Orenburg State Agrarian University | Kartashova O.L.,Russian Academy of Sciences | Sycheva M.V.,Orenburg State Agrarian University
Probiotics and Antimicrobial Proteins | Year: 2014

Classical microbiological approach and atomic force microscopy were used to evaluate the mechanisms of biological activity of antimicrobial peptides (AMPs) derived from platelets of farm animals. It is established that AMPs inhibit both Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) microorganisms. Differences revealed in the biological activity of AMP preparations obtained from the organisms of various species can be reduced to quantitative differences. While qualitative changes of bacterial cells were substantially similar, changes in the integrity of cell walls resulted in disintegration of the bacterial outer and/or cytoplasmic membranes. © 2014, Springer Science+Business Media New York.

Shcheglova L.S.,Orenburg State University | Abramova L.L.,Orenburg State Agrarian University | Maryakhina V.S.,Orenburg State University
Quantum Electronics | Year: 2013

The differences in optical and biophysical properties between the cells of mammary gland tumour extracted from tumours of different diameter are described. It is shown that the spectral and spectrokinetic properties of fluorescent probes in the cells extracted from the tumours 1 - 3 cm in diameter are essentially different. Thus, the extinction coefficient of rhodamine 6G gradually increases with the pathology development. At the same time the rate of interaction of the triplet states of molecular probes with the oxygen, diluted in the tumour cells cytoplasm, decreases with the growth of the tumour capsule diameter. The observed regularities can be due to the changes in the cell structure, biochemical and biophysical properties. The reported data may be useful for developing optical methods of diagnostics of biotissue pathological conditions. © 2013 Kvantovaya Elektronika and Turpion Ltd.

Ivanova V.Y.,Kazan Federal University | Chevela V.V.,Kazan Federal University | Bezryadin S.G.,Orenburg State Agrarian University
Russian Chemical Bulletin | Year: 2015

Complex formation of In3+ ion with citric acid in an aqueous solution was studied by pH-metric titration at the molar ratio of the reactants [In3+] : [H4Cit] = 1 : 1, 1 : 2, and 1 : 3 in a range of pH 2—10. The mathematical simulation of equilibria in an indium(iii)—citric acid system was performed using the CPESSP program package. The formation of 1 : 1, 1 : 2, and 1 : 3 indium(iii) citrate complexes with different degrees of nuclearity and protonation was established. The equilibrium constants of formation of the complexes were calculated. The predomination of the polynuclear indium(iii) citrate complexes at the equimolar metal to ligand ratio of was observed in almost the whole pH range studied (3—10). © 2015, Springer Science+Business Media New York.

Bezryadin S.G.,Orenburg State Agrarian University | Chevela V.V.,Kazan Federal University | Ajsuvakova O.P.,Orenburg State Agrarian University | Ivanova V.Y.,Kazan Federal University | Kuzyakin D.V.,Orenburg State Agrarian University
Russian Chemical Bulletin | Year: 2015

Complexation reactions in the system titanyl sulfate—(+)-tartaric acid were studied by potentiometric titration and mathematical modeling of equilibria over wide concentration ranges of the TiIV ion and the ligand at pH 1.5—10.5. Complex species present in this system were identified for the first time, their constants of formation were calculated, and the nature of species at different pH values was determined. The model proposed provides a satisfactory description of the titanium(iv) complexes in tartrate solutions over the whole concentration and pH ranges studied. The study revealed the formation of diand tetranuclear complexes, apart from mononuclear ones. It was found that TiIV tartrates are more stable than TiIV citrates of the same stoichiometry. The exception is [Ti(H3L)2]2+, which is probably due to the participation of all OH groups of tartaric acid in complexation. © 2015, Springer Science+Business Media New York.

Gusev N.F.,Orenburg State Agrarian University | Nemereshina O.N.,Orenburg State Medical Academy | Petrova G.V.,Orenburg State Agrarian University | Sychev M.V.,Orenburg State Agrarian University
Russian Journal of Biopharmaceuticals | Year: 2012

The results of the author's research of the contents of biologically active substances in medicinal plant raw material (grass) of the genus Veronica L. harvested in Urals are presented. A high content of flavonoids of flavone, phenol carboxylic acids, and tannin were determined. It was demonstrated that herbal medicines Veronica show strong antibacterial activity against Staphyloccus aureus and Escherichia coli.

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