Oral Surgery and Implantology Unit

Santiago de Compostela, Spain

Oral Surgery and Implantology Unit

Santiago de Compostela, Spain
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PubMed | University of Santiago de Compostela, University of Vigo, São Paulo State University, University Braz Cubas and 2 more.
Type: Journal Article | Journal: Acta stomatologica Croatica | Year: 2016

The aim of this study was to compare three methods of RNA extraction for molecular analysis of oral cytology to establish the best technique, considering its concentration and purity for molecular tests of oral lesions such as real-time reverse transcriptase reaction.The sample included exfoliative cytology from the oral cavity mucosa of patients with no visible clinical changes, using Orcellex Rovers BrushTrizol group revealed higher average concentration, followed by Direct-zolConsidering all aspects, concentration, purity and time spent in the procedures, the Direct-zol


Perez-Sayans M.,Institute Of Research Of Santiago Of Compostela Idis | Suarez-Penaranda J.,University of Santiago de Compostela | Gayoso-Diz P.,University of Santiago de Compostela | Barros-Angueira F.,University of Santiago de Compostela | And 2 more authors.
Medicina Oral, Patologia Oral y Cirugia Bucal | Year: 2013

Oral Squamous Cell Carcinoma (OSCC) is biologically characterized by the accumulation of multiple genetic and molecular alterations that end up clinically characterized as a malignant neoplasm through a phenomenon known as multistep. The members of the Cip/Kip family, specifically p21Waf1/CIP1, are responsible for cell cycle control, blocking the transition from phase G1 to phase S. We made a search of articles of peer-reviewed Journals in PubMed/ Medline, crossing the keywords. The goal of this paper is to determine the relationship between p21Waf1/CIP1 expression and several clinical and pathological aspects of OSCC, their relationship with p53 and HPV, as well as genetic alterations in their expression pattern, their use as a prognosis market in the evolution of precancerous lesions and their roles in anticancer treatments. The results of p21WAF1/CIP1 expression in OSCC showed mixed results in terms of positivity/negativity throughout different studies. It seems that, although p21Waf1/CIP1 expression is controlled in a p53-dependent manner, coexpression of both in OSCC is not intrinsically related. Although the presence of HPV viral oncoproteins increases p21Waf1/CIP1 levels, the small number of studies, have forced us to disregard the hypothesis that HPV infected lesions that present better prognosis are due to a p21Waf1/CIP1-dependent control. The role of p21WAF1/CIP1 as cell-cycle regulator has been well described; however, its relationship to OSCC, the clinical and pathological variables of tumors, HPV and different treatments are not entirely clear. Thus, it would be very interesting to pursue further study of this protein, which may have a significant value for the diagnosis, prognosis and therapy of this type of tumors. © Medicina Oral S. L. C.I.F. B.


Perez-Sayans M.,Oral Surgery and Implantology Unit | Reboiras-Lopez M.D.,Oral Surgery and Implantology Unit | Somoza-Martin J.M.,Oral Surgery and Implantology Unit | Barros-Angueira F.,Hospital Clinico Universitario | And 3 more authors.
Cancer Biology and Therapy | Year: 2010

Background: Oral squamous cell carcinoma (OSCC) is the most common malignancy of the oral cavity. Brush cytology has reemerged as a molecular tool for diagnosing this cancer. ATP6V1C1, one of the main genes regulating V-ATPase activity, has been implicated in metastasis and multiple drug resistance. The aim of this study was to measure ATP6V1C1 expression levels in OSCC and to evaluate the value of this test in the diagnosis and prognosis of OSCC. Results: The diff erences in ATP6V1C1 expression between patients and controls were statistically significant (Mann-Whitney U test = 26, p < 0.001). Receiver operating characteristic (ROC) curve analysis showed an area under the curve of 0.9476, with the following diagnostic indices: sensitivity, 81.25%, specificity, 93.75%; accuracy, 87.50%; positive predictive value, 92.86%; negative predictive value, 83.33%; positive likelihood ratio, 30; and negative likelihood ratio, 0.06. Material and methods: Patients with OSCC and a control group of healthy individuals were studied. The clinical and demographic variables analyzed included age, sex, smoking, tumor location and tumor stage. Brush cytology samples were obtained using a cytology brush and analyzed by real-time quantitative polymerase chain reaction for ATP6V1C1 expression. Conclusions: It was confirmed that ATP6V1C1 levels were significantly higher in patients with OSCC than in healthy controls, with expression increasing with higher tumor stage. ROC analysis showed that the measurement of ATP6V1C1 expression levels is a highly sensitive and specific diagnostic method. © 2010 Landes Bioscience.


Perez-Sayans M.,Oral Surgery and Implantology Unit | Suarez-Penaranda J.M.,Hospital Clinico Universitario Of Santiago | Barros-Angueira F.,Hospital Clinico Universitario | Diz P.G.,Complejo Hospitalario Universitario Of Santiago | And 2 more authors.
Brazilian Journal of Biology | Year: 2012

Vacuolar ATPases (V-ATPases) are present in specialized proton secretory cells in which they pump protons across the membranes of various intracellular organelles and across the plasma membrane. The proton transport mechanism is electrogenic and establishes an acidic pH and a positive transmembrane potential in these intracellular and extracellular compartments. V-ATPases have been found to be practically identical in terms of the composition of their subunits in all eukaryotic cells. They have two distinct structures: a peripheral catalytic sector (V1) and a hydrophobic membrane sector (V0) responsible for driving protons. V-ATPase activity is regulated by three different mechanisms, which control pump density, association/dissociation of the V1 and V0 domains, and secretory activity. The C subunit is a 40-kDa protein located in the V1 domain of V-ATPase. The protein is encoded by the ATP6V1C gene and is located at position 22 of the long arm of chromosome 8 (8q22.3). The C subunit has very important functions in terms of controlling the regulation of the reversible dissociation of V-ATPases.


PubMed | University of Sao Paulo, University of Vigo, São Paulo State University, Oral Surgery and Implantology Unit and University of Santiago de Compostela
Type: Journal Article | Journal: Biotechnic & histochemistry : official publication of the Biological Stain Commission | Year: 2016

We investigated the gene and protein expressions of V-type ATPase protein subunit C1 (ATP6V1C1) in cases of oral squamous cell carcinoma (OSCC) and contralateral normal mucosa in smokers, nonsmokers and former smokers. Subjects were separated into five groups of 15: group 1, smokers with OSCC; group 2, normal contralateral mucosa of OSCC patients; group 3, chronic smokers; group 4, former smokers who had stopped smoking 1 year earlier; group 5, individuals who had never smoked. Exfoliative cytology specimens from oral mucosa of smokers, former smokers and nonsmokers showed normal gene and protein expression. We found significantly greater gene expression in the OSCC group than in the nonsmoker groups. No difference in gene expression was observed between normal contralateral mucosa and nonsmoker groups, smoker and nonsmoker groups or former smoker and nonsmoker groups. We observed intense immunostaining for ATP6V1C1 protein in all cases of OSCC and weak or no staining in smoker, former smoker and nonsmoker groups. Significantly greater expression of ATP6V1C1 protein was observed in the OSCC group compared to the other groups, which supports the role of ATP6V1C1 in effecting changes associated with oral cancer. Analysis of the mucosae of chronic smokers, former smokers and the normal contralateral mucosa of patients with OSCC showed unaltered ATP6V1C1 gene and protein expression. Early stages of carcinogenesis, represented by altered epithelium of chronic smokers, had neither gene nor protein alterations as seen in OSCC. Therefore, we infer that the changes in ATP6V1C1 occur during later stages of carcinogenesis. Our preliminary study provides a basis for future studies of using ATP6V1C1 levels for detecting early stage OSCC.


Perez-Sayans M.,Institute Investigacion Sanitaria Of Santiago Idis | Suarez-Penaranda J.M.,University of Santiago de Compostela | Pilar G.-D.,Hospital Clinico Universitario Of Santiago Of Compostela | Supuran C.T.,University of Florence | And 4 more authors.
Journal of Oral Pathology and Medicine | Year: 2012

Introduction: Carbonic anhydrases (CAs), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, including tumorigenicity. Specifically, CA-IX has been primarily found in hypoxic tumor tissues. Material and methods: This is a retrospective study of tumors from the Tissue Bank of the Pathology Department of the University Hospital of Santiago de Compostela. We selected 50 oral squamous cell carcinomas (OSCCs) using Tissue Microarray (TMA) technology. The immunohistochemical study was performed to determine CA-IX expression. The resulting data were subject to statistical analysis and survival curves. Results: Of the 50 cases, 23 were detected in early stages (I and II) and 27 in advanced stages (III and IV). In the first year, almost 50% of patients in stages III-IV died, which contrasted with those patients in initial stages who registered a survival rate of 80% (P=0.019). Regarding the expression of CA-IX, nine cases (18%) were negative, 18 cases (36%) were moderate, while 23 cases (46%) were intense. Tumors in stages I-II showed a positivity of 52.6%; however, in advanced stages, the percentage reached 95.5% (P=0.002). Regarding CA-IX expression and survival, patients with tumors with strong staining had a lower average survival time (13.8months) than patients with negative or weak-moderate staining (33.4 and 32.8months, respectively), log-rank=6.1, P value=0.0484. Conclusions: Early diagnosis of these tumors is essential to improve patient survival. CA-IX expression augments with increasing tumor stage, probably related with the degree of hypoxia; thus, its measurement can be used as a prognostic factor. © 2012 John Wiley & Sons A/S.


Perez-Sayans M.,Institute Investigacion Sanitaria Of Santiago Idis | Supuran C.T.,University of Florence | Pastorekova S.,Slovak Academy of Sciences | Suarez-Penaranda J.M.,Hospital Clinico Universitario Of Santiago | And 5 more authors.
Journal of Oral Pathology and Medicine | Year: 2013

Tumoral microenvironments play a key role in the evolution of solid tumors. Tumor hypoxia is actively involved in the promotion of genetic instability, the invasive capacity of tumor cells, metastasis, and a worsening of the clinical evolution. Endogenous hypoxia markers are controlled by hypoxia-related genes, formed by HIF-1, which is related to several target genes that involve the energy metabolism, angiogenesis, and transmembrane carbonic anhydrases (CAs), mainly CA-IX that is one of the tumor-related carbonic anhydrases. The goal of this paper is to establish the role of CA-IX as a hypoxia marker in OSCC, while analyzing its expression in this type of tumors and its relationship with several clinical and pathological parameters and prognosis, evaluating its relationship with angiogenesis, other hypoxia markers, and clarifying its role in chemotherapy and radiotherapy resistance. © 2012 John Wiley & Sons A/S.


Vidal-Real C.,Oral Surgery and Implantology Unit | Perez-Sayans M.,Institute Investigacion Sanitaria Of Santiago Idis | Suarez-Penaranda J.-M.,University of Santiago de Compostela | Gandara-Rey J.-M.,Oral Surgery and Implantology Unit | Garcia-Garcia A.,Institute Investigacion Sanitaria Of Santiago Idis
Medicina Oral, Patologia Oral y Cirugia Bucal | Year: 2015

Background: Osteonecrosis of the jaw (ONJ) is a destructive bone process in patients undergoing bisphosphonate therapy and it is modulated by local and systemic factors. The purpose of this article is to determine the prevalence of ONJ in patients who have undergone intravenous bisphosphonate therapy, and relate the risk factors described to establish a protocol to reduce the risk of developing ONJ. Material and Methods: We performed a retrospective study on 194 patients treated with IV bisphosponates, analyzing clinical and pathological variables. Results: The prevalence of ONJ was 12.9 %. The most remarkable complication was pain, which was reported by 80% of patients. The average age of the patients undergoing bisphosphonate therapy was 68.91 years. Most of non-diabetic patients did not develop ONJ (92.3%) (p=0.048). During bisphosphonate therapy, 3.1% of patients underwent extractions in the same percentage in the maxilla and in the mandible; all of which, except for one patient, developed ONJ (p<0.001). In regards to the periodontal state, 94.3% of patients without periodontal problems did not develop ONJ (p=0.001). Almost 50% of the necrosis were located unifocally on the mandible (p<0.001). The number of affected patients and the aggressiveness of the disease increased significantly three years after starting treatment (p<0.001). Conclusions: Etiology still is a controversial issue and we should focus on known risk factors, such as the development of surgical procedures in patients undergoing bisphosphonate therapy, especially in patients who have already started their treatment, a group in which ONJ prevalence increases. Moreover, a bad periodontal state in these patients is also an important risk factor, and the control of diabetes reduces it. Due to the above, all patients should be diagnosed and educated in oral hygiene prior to treatment, performing periodical maintenance, to detect possible traumatisms and periodontal infection as soon as possible. © 2015, Medicina Oral, Patologia Oral y Cirugia Bucal. All rights reserved.


Perez-Sayans M.,Oral Surgery and Implantology Unit | Perez-Sayans M.,Institute Investigacion Sanitaria Of Santiago Idis | Pilar G.-D.,Institute Investigacion Sanitaria Of Santiago Idis | Pilar G.-D.,Hospital Clinico Universitario Of Santiago Of Compostela | And 6 more authors.
Journal of Oral Pathology and Medicine | Year: 2012

A micro RNA (miRNA) is a single-stranded endogenous, non-coding RNA, with length ranging between 18 and 24 nucleotides and the ability of regulating the expression of other genes on a post-transcriptional level by means of various processes, degradation or repression of target mRNA. miRNAs play a crucial role in regulating fundamental processes such as cell cycle, differentiation and apoptosis; thus, their deregulation can affect normal cell growth and development, and even participate in carcinogenesis. The goals of this paper are: to outline the formation and functions of miRNAs; to determine their role in oral squamous cell carcinoma; to analyze the different miRNAs described and their roles as oncogenes or tumor suppressor genes, depending on their overexpression or subexpression; to describe the different polymorphisms and epigenetic alterations identified; and to determine their role in multidrug resistance. © 2011 John Wiley & Sons A/S.


Garcia M.P.-S.,Oral Surgery and Implantology Unit | Garcia-Garcia A.,Oral Surgery and Implantology Unit
Methods in Molecular Biology | Year: 2012

Epigenetics studies and defines inherited changes in gene expression that are not encoded in the DNA sequence. The most studied epigenetic change in mammalian DNA is cytosine methylation in CpG dinucleotide areas. The other main group in epigenetic changes includes the posttranslational modifications of histones, mainly phosphorylation, deacetylation changes, and in the ubiquitinylation status. Oral squamous cell carcinoma is the most common malignancy of the oral cavity, and epigenetic changes are very common, as described in this chapter. Alterations in the DNA methylation status resulting from exposure to environmental stress agents have been documented even before birth. Although many epigenetic markers are potentially reversible, the mechanism still remains unclear and many epigenetic changes persist across cell lines and the life of the organism. © 2012 Springer Science+Business Media, LLC.

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