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Zanon-Moreno V.,University of Valencia | Zanon-Moreno V.,Ophthalmology Research Unit Santiago Grisolia | Zanon-Moreno L.,University of Valencia | Ortega-Azorin C.,University of Valencia | And 11 more authors.
Clinical and Experimental Ophthalmology | Year: 2015

Background: To investigate the possible association of the rs2165241 polymorphism (C>T) in LOXL1 gene with the risk of primary open-angle glaucoma in a Mediterranean population. Methods: The analysis of genetic polymorphisms was performed by standard TaqMan allelic discrimination technique, using a 7900HT Sequence Detection System (Applied Biosystems). Results: In a recessive genetic model, the T allele of the rs2165241 polymorphism was significantly associated with the risk of primary open-angle glaucoma (TT vs. CC: odds ratios=2.19, 95% confidence interval=[1.33-3.62]). After multivariate logistic regression model adjusted by age and weight, the magnitude of the association decreased but remained statistically significant (TT vs. CC: odds ratios=2.07, 95% confidence interval=[1.20-3.57]). Conclusion: This polymorphism seems to be associated with high risk for primary open-angle glaucoma in a Mediterranean population. © 2014 Royal Australian and New Zealand College of Ophthalmologists.

Dolz-Marco R.,Polytechnic University of Valencia | Gallego-Pinazo R.,Polytechnic University of Valencia | Pinazo-Duran M.D.,Ophthalmology Research Unit Santiago Grisolia | Pinazo-Duran M.D.,University of Valencia | And 3 more authors.
PLoS ONE | Year: 2014

Purpose: The purpose of the present study was to evaluate the retinal toxicity of a single dose of intravitreal docosahexaenoic acid (DHA) in rabbit eyes over a short-term period. Methods: Sixteen New Zealand albino rabbits were selected for this pre-clinical study. Six concentrations of DHA (Brudy Laboratories, Barcelona, Spain) were prepared: 10 mg/50 μl, 5 mg/50 μl, 2′5 mg/50 μl, 50 μg/50 μl, 25 μg/50 μl, and 5 μg/ 50 μl. Each concentration was injected intravitreally in the right eye of two rabbits. As a control, the vehicle solution was injected in one eye of four animals. Retinal safety was studied by slit-lamp examination, and electroretinography. All the rabbits were euthanized one week after the intravitreal injection of DHA and the eyeballs were processed to morphologic and morphometric histological examination by light microscopy. At the same time aqueous and vitreous humor samples were taken to quantify the concentration of omega-3 acids by gas chromatography. Statistical analysis was performed by SPSS 21.0. Results: Slit-lamp examination revealed an important inflammatory reaction on the anterior chamber of the rabbits injected with the higher concentrations of DHA (10 mg/50 μl, 5 mg/50 μl, 2′5 mg/50 μ) Lower concentrations showed no inflammation. Electroretinography and histological studies showed no significant difference between control and DHA-njected groups except for the group injected with 50 μg/50 μl. Conclusions: Our results indicate that administration of intravitreal DHA is safe in the albino rabbit model up to the maximum tolerated dose of 25 μg/50 μl. Further studies should be performed in order to evaluate the effect of intravitreal injection of DHA as a treatment, alone or in combination, of different retinal diseases. © 2014 Dolz-Marco et al.

Garcia-Medina J.J.,Huercal Overa Hospital | Garcia-Medina J.J.,University of Murcia | Garcia-Medina J.J.,Ophthalmology Research Unit Santiago Grisolia | Garcia-Medina M.,Torrecardenas Hospital | And 7 more authors.
Acta Ophthalmologica | Year: 2015

Purpose To evaluate the effect of oral antioxidant supplementation (OAS) on primary open-angle glaucoma (POAG) over a 2-year follow-up period. Patients and methods In this open-label, randomized controlled trial, 117 eyes of 117 patients with mild or moderate POAG and intraocular pressure under control with topical antiglaucoma medications were recruited and randomly divided into three groups according to supplementation: (1) OAS with (ICAPS R® - Alcon Laboratories, n = 26); (2) OAS without ω-3 fatty acids (OFTAN MACULA® - Laboratorios Esteve, n = 28); and (3) a control group without OAS (n = 63). They all underwent visual field (VF) tests (Humphrey 24-2) and scans using a Fourier-domain optical coherence tomography (FD-OCT) device (RTVue-100) at the beginning of the study and 2 years later. Mean deviation (MD), standard pattern deviation (PSD), peripapillary retinal nerve fibre layer (RNFL) and macular ganglion cell complex (GCC) parameters were considered for the analysis. Patients were also classified according to MD deterioration (fast deterioration vs. slow deterioration). Results Visual field global indices, peripapillary RNFL thickness and macular GCC thickness showed no differences among the groups at the beginning and end of the follow-up. Besides all the comparisons among groups for differences before and after the follow-up of the MD, PSD, RNFL and GCC parameters were also non-significant. The proportions of patients according to MD deterioration were similar among the groups and subgroups (p > 0.05 for all the comparisons). Conclusion Oral antioxidant supplementation with or without ω-3 fatty acids does not appear useful as an adjuvant treatment of mild/moderate POAG in the short term. © 2014 Acta Ophthalmologica Scandinavica Foundation. Published by John Wiley & Sons Ltd.

Garcia-Medina J.J.,University of Murcia | Garcia-Medina J.J.,Ophthalmology Research Unit Santiago Grisolia | Del-Rio-Vellosillo M.,University of Murcia | Zanon-Moreno V.,Ophthalmology Research Unit Santiago Grisolia | And 5 more authors.
Arquivos Brasileiros de Oftalmologia | Year: 2014

We describe a patient with acute scleral dellen (SD) after pterygium excision with simple conjunctival closure. In addition, we present a PUBMED review on the medical literature on early SD after pterygium surgery. This case describes a 45-year-old man who presented with severe SD, 7 days after pterygium surgery with minimal cauterization of episcleral vessels and simple conjunctival closure. No other adjunctive therapy was used intraoperatively. The patient refused conjunctival flap coverage of the lesion. Therefore, medical treatment consisted of antibiotic ointment, patching, and daily follow-up. After 7 days, the patching was changed for intensive ocular lubrication. Five weeks later, the surrounding conjunctiva had completely covered the affected sclera. To the best of our knowledge, this is the first report of early SD following pterygium excision and simple conjunctival closure with no other adjunctive therapy. When performing pterygium excision with conjunctival coverage of the sclera, a close follow-up is recommended to rule out wound dehiscence and SD, even when surgical wound closure is considered to prevent SD. If this complication is detected, the treatment can be conservative.

Garcia-Medina J.J.,University of Murcia | Garcia-Medina J.J.,Ophthalmology Research Unit Santiago Grisolia | Garcia-Medina J.J.,Institute Salud Carlos III | Del Rio-Vellosillo M.,University of Murcia | And 12 more authors.
BioMed Research International | Year: 2015

The visual outcome obtained after cataract removal may progressively decline because of posterior capsular opacification (PCO). This condition can be treated by creating an opening in the posterior lens capsule by Nd:YAG laser capsulotomy. PCO optical imperfections cause several light reflection, refraction, and diffraction phenomena, which may interfere with the functional and structural tests performed in different ocular locations for the diagnosis and follow-up of ocular disease, like macular and optic nerve diseases. Some parameters measured by visual field examinations, scanning laser polarimetry, and optical coherence tomography (OCT) have changed after PCO removal. Imaging quality also changes following capsulotomy. Consequently, the results of ancillary tests in pseudophakic eyes for studying ocular diseases like glaucoma or maculopathies should be correlated with other clinical examinations, for example, slit-lamp biomicroscopy or funduscopy. If PCO is clinically significant, a new baseline should be set for future comparisons following capsulotomy when using automated perimetry and scanning laser polarimetry. To perform OCT in the presence of PCO, reliable examinations (considering signal strength) apparently guarantee that measurements are not influenced by PCO. © 2015 Jose Javier Garcia-Medina et al.

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