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Shapoval L.M.,Oo Bogomolets Institute Of Physiology | Prylutska S.V.,Taras Shevchenko National University | Kotsyuruba A.V.,Oo Bogomolets Institute Of Physiology | Dmitrenko O.V.,Oo Bogomolets Institute Of Physiology | And 3 more authors.
Materialwissenschaft und Werkstofftechnik

Effect of water dispersible single-walled carbon nanotubes on the medullary cardiovascular control was studied in normotensive (control) and spontaneously hypertensive rats using physiological and biochemical approaches. We have shown for the first time the possible contribution of nitric oxide (NO) to single-walled carbon nanotube-induced effects in the cardiovascular nuclei of the medulla oblongata (the lateral reticular nucleus and nucleus ambiguous). The data of biochemical experiments indicate that single-walled carbon nanotubes added to the medulla oblongata homogenates induce an enhancement of constitutive nitric oxide-synthase (cNOS) activity, which is mostly neuronal nitric oxide-synthase (nNOS) in the brain, and an increase in the level of nitrite (NO- 2), a stable nitric oxide metabolite which serves nitric oxide bioavailability marker. In physiological experiments, it has been revealed that effects of single-walled carbon nanotube injection in the cardiovascular nuclei under study are attenuated after preliminary nNOS inhibiting. An analysis of single-walled carbon nanotube-induced changes in the levels of malonic dialdehyde, an oxidative stress marker, as well as the levels of reactive nitrogen species (pools of nitrate anions), and the activity of Ca2+-independent inducible nitric oxide synthase (iNOS) has indicated that single-walled carbon nanotubes used in the study do not promote oxidative and nitrosative stress in control and spontaneously hypertensive rats. © 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim February 2016. Source

Reznikov A.G.,Ukrainian Academy of Sciences | Salivonyk O.A.,Ukrainian Academy of Sciences | Sotkis A.G.,Oo Bogomolets Institute Of Physiology | Sotkis A.G.,Ukrainian Academy of Sciences | And 2 more authors.
Experimental Oncology

In recent years gold nanoparticles (AuNPs) have received considerable attention for various biomedical applications including diagnostics and targeted drug delivery. However, more research is still needed to characterize such aspects of their use in clinical oncology as permeability, retention and functional effect on tumor cells. Aims: This study was designed to describe the effect of non-functionalized AuNPs on LNCaP prostate cancer cells growth. Material and Methods: LNCaP cells were cultured in RPMI-1640 medium containing AuNPs covered by polyvinylpyrrolidone of average size 26.4 nm (10.0 μg/ml). Counts of cells were calculated and their morphology was examined. Results: AuNPs conglomerates have been visualized in cultured cells. After 4-day incubation in presence of AuNPs significant retardation of LNCaP cells growth was observed both in 5α-dihydrotestosterone stimulated and non-stimulated cultures. No morphological changes of live LNCaP cells were seen in any experiment. Conclusion: Given absence of morphological changes in live cells and dribble and relatively constant numbers of dead cells, it was concluded that inhibitory effect of AuNPs on LNCaP cells growth was caused by alterations of proliferation. Copyright © Experimental Oncology, 2015. Source

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