Onkotec Gmbh

Waidhofen an der Thaya, Austria

Onkotec Gmbh

Waidhofen an der Thaya, Austria
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Edetsberger M.,Onkotec Gmbh | Knapp M.,Onkotec Gmbh | Gaubitzer E.,Onkotec Gmbh | Miksch C.,Max F Perutz Laboratories | And 2 more authors.
Journal of Inclusion Phenomena and Macrocyclic Chemistry | Year: 2011

In a comprehensive picture of inclusion complex formation of the highly fluorescent dye coumarin-6 (C6) and betacyclodextrin (beta-CD), which was obtained using various fluorescence spectroscopic methods, it was demonstrated that up to three beta-CD rings can thread on the rod like dye molecule. Interaction of coumarins and modified coumarins with cellular organelles or proteins has been reported in several publications. Especially 7-amino-coumarins are characterized by unique properties like high fluorescence quantum yield and are thus already used successfully in different areas, like staining of fluorescent nanoparticles. We could show that Coumarin-6 made soluble by complexation with beta-cyclodextrin is able to stain eukaryotic cells specifically dependent on their origin and cellular behaviour. The staining reaction is independent from pH, is photo stable, and shows no cross talk with proteins in the cytoplasm and other staining procedures or erythrocytes. Staining with coumarin 6/cyclodextrin complexes can thus be used for fast discrimination of different cell types. Importantly, it could be shown that the ideal staining reaction is dependent on the stoichiometry of the complex-formation. © 2010 Springer Science+Business Media B.V.

Agency: European Commission | Branch: FP7 | Program: CP-FP | Phase: HEALTH.2012.1.2-1 | Award Amount: 4.60M | Year: 2012

With the advent of Omics technologies capable of profiling substantial fractions of molecular entities from the genome down to the metabolome level a significant boost in understanding disease pathophysiology, consequently providing tailored diagnostics and therapy, was expected. With impressive results for selected diseases, cancer still sees significant shortcomings. One reason for this clinical situation is certainly the apparent heterogeneity of cancers, manifesting in clinical presentation and outcome, but also on the personalized molecular level. Within DIPROMON we selected bladder cancer as a prototypical example for the need of personalization efforts in therapy, and we plan of using this clinically highly challenging disease phenotype for establishing a general workflow for enabling personalization strategies towards rational decision on tailoring therapeutic interventions for defined patient groups. DIPROMON on its basis follows a computational Systems Biology approach for delineating statistical rules for patient segmentation, resting on patient-specific biomarker profiles interpreted in tight integration with patient-specific clinical data. DIPROMON will establish a workflow including quality control aspects for i) selecting biomarker panels for given clinical phenotypes, ii) providing modular instrumentation for measuring the profiles in a clinical setting, and iii) deriving statistics-driven rules for patient segmentation regarding optimal therapy. DIPROMON will finally validate the concept in bladder cancer.

Muhlberger M.,Functional Surfaces and Nanostructures | Fachberger H.,Functional Surfaces and Nanostructures | Bergmair I.,Functional Surfaces and Nanostructures | Rohn M.,Functional Surfaces and Nanostructures | And 12 more authors.
Proceedings of SPIE - The International Society for Optical Engineering | Year: 2012

The NILaustria research project cluster consists of 8 individual research projects and aims to improve nanoimprint lithography in an application driven approach. The cluster is presented as well as highlights from the projects, e.g. the replication of 12.5nm half pitch features using working stamp copies, topics from organic electronics, metamaterials and SiGe technology. An outlook on the new activities is given. © 2012 SPIE.

Smetana W.,Vienna University of Technology | Balluch B.,Vienna University of Technology | Balluch B.,OnkoTec GmbH | Atassi I.,Vienna University of Technology | And 7 more authors.
Communications in Computer and Information Science | Year: 2010

A 3-dimensional mesofluidic biological monitoring module has been successfully designed and fabricated using a low-temperature co-fired ceramic (LTCC) technology. This mesofluidic device consists of a network of micro-channels, a spherical mixing cavity and measuring ports. A selection of appropriate commercially available ceramic tapes has been chosen with regard to their biocompatibility performance. Specific processing procedures required for the realization of such a complex structure are demonstrated. Three dimensional numerical flow simulations have been conducted to characterize the concentration profiles of liquids at a specific measuring port and verified by experiment. © 2010 Springer-Verlag Berlin Heidelberg.

Franz M.,Vienna University of Technology | Atassi I.,Vienna University of Technology | Maric A.,University of Novi Sad | Balluch B.,OnkoTec GmbH | And 4 more authors.
Proceedings of the International Spring Seminar on Electronics Technology | Year: 2012

This paper provides new material property measurement results of the ceramic film material CERAMTAPE GC (manufactured by CERAMTEC GMBH), a commercially available LTCC (Low Temperature Co-fired Ceramics) tape for electronic and microfluidic applications in harsh environment. Following material properties are presented: x-y-z-shrinkage dependent on the lamination pressure, density, weight loss, surface parameters of the green and co-fired tape, thermal properties, Young's modulus, permittivity, chemical composition, and bio-compatibility. © 2012 IEEE.

Shahzad A.,University of Vienna | Knapp M.,OnkoTec GmbH | Edetsberger M.,OnkoTec GmbH | Puchinger M.,University of Vienna | And 2 more authors.
Applied Spectroscopy Reviews | Year: 2010

Cancer is one of the big killers of world population. The majority of cancers are diagnosed at a late stage, making a cure almost impossible. Fluorescence spectroscopy is an emerging diagnostic tool for various medical diseases including premalignant and malignant lesions. Fluorescence spectroscopy is a noninvasive technique and has been applied successfully for the diagnosis of multisystem cancers with high sensitivity and specificity. Fluorescence spectroscopy minimizes the need for repetitive biopsy, which is routine practice for cancer patient follow-up. But there are many aspects of this new diagnostic technique that should be discussed in future research to overcome limitations and challenges faced by this technique for diagnosis of cancers.

Ortner A.,Medical University of Vienna | Wernig K.,Universittsplatz 1 | Kaisler R.,Onkotec GmbH | Edetsberger M.,Onkotec GmbH | And 4 more authors.
Journal of Drug Targeting | Year: 2010

The receptors for vasoactive intestinal peptide (VIP), VPAC1-, VPAC2-, and PAC1-receptor are overexpressed by various tumor cells. VIP can target these receptors and transport conjugates into the cell. However, the use of VIP for tumor cell targeting is hampered by the peptides short half-lives due to enzymatic degradation. Because protamine-based nanoparticles (proticles) protect the peptide and serve as peptide depot, we explored the potential of proticles as carrier for VIP-conjugated molecules. The VIP-loaded proticles were stable as shown by Fluorescence Correlation Spectroscopy. With Confocal Laser Scanning Microscopy, we observed VIP-loaded proticles to specifically target the tumor cells. The cell binding triggered the substance release and conjugate internalization of VIP-Cy3 in vitro and ex vivo by human tumors. We observed VIP releasing proticle depots distributed in rat tissue and human tumors. Our findings warrant further studies to explore the proticles potential to enable peptide-mediated targeting for in vivo and clinical applications. © 2010 Informa UK Ltd.

A method for qualitative and/or quantitative analysis of tumor cells, including the following steps: a) generating a dye complex from an aminocoumarin and cyclodextrin; b) mixing the tumor cells with the dye complex prepared in step a); c) incubating the cells with the dye complex prepared in step a); d) analyzing the tumor cells by means of fluorescence microscopy and/or fluorescence spectrometric analysis.

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