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Oniris and Nantes University Hospital Center | Date: 2017-05-17

The present invention relates to the use of insulin-producing cells encapsulated in silanized hydroxypropyl methylcellulose (Si-HPMC) for the treatment of type 1 diabetes. Methods and kits are also provided for restoring and/or maintaining euglycemia in type 1 diabetic patients and in type 1 prediabetic patients.

The present invention relates to microRNAs (miR-7a, miR-215 and miR-375) for use in the in vitro diagnosis of insulitis and the in vitro prognosis of future type 1 diabetes development. The invention also relates to miR-specific primers and probes, kits comprising these primers and/or probes, and in vitro methods of diagnosis and prognosis using these primers and/or probes. Also provided are methods for assessing the effectiveness of a therapeutic treatment for insulitis or of a treatment for preventing type 1 diabetes onset, as well as methods of screening for candidate compounds potentially useful in the treatment for insulitis or in the prevention of type 1 diabetes onset.

Schoebitz M.,ONIRIS | Schoebitz M.,Austral University of Chile | Simonin H.,ONIRIS | Poncelet D.,ONIRIS
Journal of Microencapsulation | Year: 2012

This work deals with optimising the cell survival of rhizobacteria encapsulated in alginate beads filled with starch. Immobilisation of rhizobacteria was done by dripping alginate-starch solution mixed with rhizobacteria into a calcium solution. Beads were analysed based on matrix formulation, bacteria growth phase, osmoprotectants and nature of calcium solution. Maximum cell recovery was obtained on Raoultella terrigena grown in medium supplemented with trehalose and calcium gluconate as gelling agent. Furthermore, dried beads containing Azospirillum brasilense presented 76 of viable cells after one year of storage. The survival of rhizobacteria during the bioencapsulation process can be improved by incorporating starch on beads composition, varying the growth phase of cells and using trehalose in growth culture medium. This work provides a selection of appropriate methods to improve the surviving rate of encapsulated cells during their production and long-term storage (∼1 year at 4°C). © 2012 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

Anon M.C.,Research Center sarrollo En Criotecnologia Of Alimentos | De Lamballerie M.,ONIRIS | Speroni F.,Research Center sarrollo En Criotecnologia Of Alimentos
Innovative Food Science and Emerging Technologies | Year: 2011

In the present work the effect of high pressure (HP) treatment in the presence of NaCl on the thermal behavior of soybean proteins was analyzed by differential scanning calorimetry. The thermograms obtained have shown that NaCl addition increased the thermal stability - increase in temperatures of denaturation (Td) - of both glycinin and β-conglycinin. HP treatments increased thermal stability of glycinin, but decreased that of β-conglycinin. High NaCl concentrations decreased (in glycinin) or inverted (in β-conglycinin) the effects of HP on thermal stability. Cooperativity of denaturation of glycinin was enhanced by NaCl and HP. Cooperativity of denaturation of β-conglycinin was enhanced by HP and also by NaCl at 0.2 mol/L but decreased with the combination of treatments. Salt addition increased the enthalpy, ΔH, of denaturation of glycinin and β-conglycinin, being this effect stronger on glycinin. HP treatment provoked the denaturation of both protein fractions. The presence of NaCl protected glycinin against HP-denaturation at any assayed salt concentration and pressure level, while β-conglycinin was only protected at 200 and 400 MPa, but was more denaturated at 600 MPa in the presence of 0.6 mol/L of NaCl. Industrial relevance: The knowledge provided by this work may be useful in the handling of high pressure-treated food with high NaCl content (e.g. meat emulsions, smallgoods) where soybean proteins are used as additives, in order to choose high pressure values to achieve their denaturation or predict the effects of ulterior thermal treatments. Thus, this knowledge may be useful to increase the use of high pressure in food industry. © 2011 Elsevier Ltd. All rights reserved.

Chartier C.,ONIRIS | Mercier P.,Anses Laboratoire Of Niort | Pellet M.-P.,Anses Laboratoire Of Niort | Vialard J.,Anses Laboratoire Of Niort
Veterinary Journal | Year: 2012

The effect of an inactivated paratuberculosis vaccine on the diagnosis of tuberculosis (TB) in goats was investigated in a herd with a history of clinical paratuberculosis but which was free of TB. Cohorts of animals in 2006, 2008 and 2009, were vaccinated once at 1 month of age, and 50% of the 2006 cohort served as unvaccinated controls. The goats were aged 8 months, 20 months and 3.5 years old at the time of the survey. All animals were assessed using a single intradermal injection of bovine tuberculin purified protein derivative (PPD) (SID test), or using both bovine and avian PPD (CID test). An interferon (IFN)-γ assay using both bovine and avian PPD was carried out on the 2006 cohort and was interpreted according to three different 'cut-off' points. No unvaccinated (control) animals tested positive to any of the assays, confirming that the herd was TB-free. The SID test had a low specificity in vaccinated animals at 8 and 20 months of age, whereas the CID test demonstrated 100% specificity in animals ≥20 months-old. The specificity of IFN-γ assay was less than maximal for vaccinated animals 3.5 years old as small numbers of false positives were detected, although this depended on the chosen cut-off point. The study findings demonstrate that the use of an inactivated paratuberculosis vaccine in goats <1 month-old in a TB-free herd does not result in false positives to a CID test for TB when performed in animals ≥20 months-old. © 2011.

Le Velly R.,Montpellier SupAgro | Dufeu I.,Oniris
Journal of Rural Studies | Year: 2016

The aim of this article is to show Actor-Network Theory's (ANT) potential for accounting for the alternative-conventional hybridity of alternative food networks. A review of the literature shows that this has not yet been done. Consequently, this article proposes to fill this gap with findings from ANT research regarding such notions as "market devices," "market mediation," and "market agencements." The theory is backed up by an analysis of a local food system involving five small fishermen and the delivery of fish to 1500 households in the area around Nantes in France. Seeing this local food system as a "market agencement," i.e., a sociotechnical arrangement capable of market action, makes it possible to underscore the many hybridities that compose alternative food networks: those of human, material, and natural entities; the local and global scales; and production and consumption; but also that of alternative and conventional actors and devices. © 2015 Elsevier Ltd.

Laffont J.-L.,DuPont Pioneer | Wright K.,DuPont Pioneer | Hanafi M.,ONIRIS
Crop Science | Year: 2013

Biplots have been widely used in recent years for the analysis of multi-environment trials through the genotype plus genotype × environment (GGE) biplot analysis or through the additive main effects and multiplicative interaction analysis. Sometimes the environments are structured in blocks of environments, for example, regions or years. In this paper, we propose a new biplot technique for describing genotype × environment interactions that takes into account this additional information. We call it a GGB biplot for genotype plus genotype × block of environments biplot. This biplot has interesting geometrical properties that will be covered in this paper. Similarly to what was done for the GGE biplot (Laffont et al., 2007), we also establish a link between the partitioning of the total sum of squares provided by the GGB biplot and the partitioning provided by the analysis of variance. Simulated and real data are used to illustrate this new biplot. © Crop Science Society of America.

Blancou P.,ONIRIS
Methods in molecular biology (Clifton, N.J.) | Year: 2011

Heme oxygenase-1 (HO-1) is one of the three isoforms of the heme oxygenase enzyme that catabolyzes the degradation of heme into biliverdin with the production of free iron and CO. HO-1 is induced by its substrate and by other stimuli, including agents involved in oxidative stress and proinflammatory cytokines as well as several anti-inflammatory stimuli. A growing body of evidence points toward the capacity of this molecule to inhibit immune reactions and the pivotal role of HO-1 in inflammatory diseases. We will first review the physiological role of HO-1 as determined by the analysis of HO-1-deficient individuals. This will be followed by an examination of the effect of HO-1 within immunopathological contexts such as immune disorders (autoimmunity and allergy) or infections. A section will be devoted to the use of an HO-1 inducer as an immunosuppressive molecule in transplantation. Finally, we will review the molecular basis of HO-1 actions on different immune cells.

The invention relates to a strain of Lactobacillus sakei called Lactobacillus sakei LHIS2885 strain, and to a strain derived from L. sakei LHIS2885. The invention also relates to the use of a strain of L. sakei, preferably the strain LHIS2885 or a strain derived from LHIS2885, for the preservation of food products, preferably products from the sea.

Bite trays (10, 11) having lateral cylindrical pivots (30) articulating two connecting rods (20) in propulsion, the front fastening elements (30a) operate according to a transverse elastic click-lock system and the rear connecting-rod fastening elements (30b) operate according to an open-ring radial locking system, in order to increase the operating safety under all circumstances.

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