Suhara W.,Oncorex Inc. |
Okuzawa T.,Oncorex Inc. |
Okuzawa T.,Chiyoda Corporation |
Koide H.,Oncorex Inc. |
And 8 more authors.
Journal of Food Biochemistry | Year: 2011
A saponin-enriched soybean extract was tested for agonistic activities against a series of nuclear receptors including peroxisome proliferator-activated receptors (PPARs) and cholesterol metabolism-related, retinoid-related, hormone-related and detoxification-related receptors as well as the detoxification-related transcription factor Nrf-2 to elucidate its mechanism of action. Reporter gene assays were used to measure the activities of the receptors and Nrf-2. The extract was found to increase the activities of PPARα, PPARδ and PPARγ, liver X receptor (LXR)α and LXRβ, estrogen receptor (ER)α and Nrf-2 dose-dependently. At 500μg/mL, it enhanced the activity of PPARα, PPARδ and PPARγ, LXRα and LXRβ, ERα and Nrf-2 2.7-, 6.5-, 9.9-, 5.2-, 2.9-, 20.1- and 9.2-fold, respectively, more than the vehicle control. It also dose-dependently enhanced the expression of acyl CoA synthetase and promoted the differentiation of 3T3-L1 cells. Pharmacological effects of soyasaponins and the saponin-enriched soybean extract including the ability to increase the activities of nuclear receptors and Nrf-2 were discussed. © 2010 Wiley Periodicals, Inc.