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Limoges, France

Giraud S.,Oncomedics | Loum E.,Oncomedics | Bessette B.,Oncomedics | Fermeaux V.,Service danatomopathologie | Lautrette C.,Oncomedics
Anticancer Research | Year: 2011

Background: Breast cancer is the most widely spread cancer in the world, attracting much research and individualized tumour response testing (ITRT) methods are now used to individualize patient chemotherapeutic administrations. A new ITRT method was developed with optimized processing. Materials and Methods: Breast tumour fragments were separated and the cells seeded in a foetal calf serum-free defined medium. After various chemotherapeutic treatments, cytotoxicity was determined by cell death detection with calcein acetoxymethyl and ethidium homodimer labelling. Results: The culture medium allowed breast tumour cell proliferation in culture, while preventing fibroblastic cell survival. Moreover, the cell death analysis gave rise to a chemoresistance profile called an Oncogramme, with statistically significant values. Conclusion: The Oncogramme is a new ITRT method which can predict patient cell sensitivities to chemotherapeutics and should be validated by a new phase I clinical trial.

Melin C.,University of Limoges | Lacroix A.,University of Limoges | Lalloue F.,University of Limoges | Pothier A.,University of Limoges | And 11 more authors.
Journal of Chromatography A | Year: 2013

SdFFF is now commonly used for cell sorting. Nevertheless, as with many other separation methods, SdFFF Hyperlayer elution leads (1) to sample dilution resulting in cell loss which could restrict further use; and (2) to a high output flow rate impacting detector sensitivity and selectivity. In order to limit these problems, we proposed modifications of the SdFFF separation channel consisting both in downscaling and the insertion of an outlet stream splitter. This last system corresponded to a strip which divides the flow rate output into two parts, one containing concentrated cells in a reduced volume and flow rate, the other containing the excess mobile phase useless for further cell manipulation, detection and characterization. For the first time we have shown that splitter implementation and downscaling respected channel flowing and resulted in Hyperlayer elution of around 95% of cells in less than 50% of input flow rate. Improved cell sorting was demonstrated by enrichment (~10 times) of cancer stem cells from WiDr cells with two times less quantity of injected cells. © 2013 Elsevier B.V.

Landoulsi A.,CNRS XLIM Research Institute, Limoges | Zhang L.Y.,CNRS XLIM Research Institute, Limoges | Dalmay C.,CNRS XLIM Research Institute, Limoges | Lacroix A.,University of Limoges | And 7 more authors.
IEEE MTT-S International Microwave Symposium Digest | Year: 2013

This paper presents an improved design of resonant biosensor, dedicated to dielectric analysis on biological cells at microwave frequencies. Such sensor uses the capability of microwaves to penetrate inside biological cells in order to interact with their intracellular content. Hence, individual dielectric properties of the cell cytoplasm can be known and then used as a signature of the cell pathological state (living or dead, malignant or safe...). In this paper is introduced a continuously tunable frequency sensor prototype, able to perform an accurate dielectric analysis over at least a 1GHz bandwidth while keeping enough sensitivity to detect and analyze a single cell. As a proof of concept, permittivity measurements have been led on calibrated size polystyrene beads: achieved results show good agreement with expected permittivity values. Finally experiments on Glioblastoma cells will be presented. © 2013 IEEE.

Giraud S.,Oncomedics | Bounaix Morand Du Puch C.,Oncomedics | Fermeaux V.,University of Limoges | Guillaudeau A.,University of Limoges | Lautrette C.,Oncomedics
Anticancer Research | Year: 2012

Background: Among targeted therapies, Herceptin is a monoclonal antibody successfully used on patients with breast cancer expressing Human Epidermal Growth Factor Receptor-2 (HER2 receptors). Oncogramme is a method developed to predict anticancer activity of molecules and thus individualize chemotherapeutic strategies. Before this ex vivo test enters clinical validation, it was desirable to correlate breast cancer cell responses to Herceptin observed through Oncogramme with HER2 expression by these cells. Materials and Methods: Breast tumour fragments were dissociated and obtained cells were cultured in defined medium. After Herceptin treatment, cytotoxicity was detected by cell death analysis, and responses compared to tumour HER2 status were determined by pathologists. Results: Cell responses to increasing doses of Herceptin obtained with Oncogramme were in correlation with HER2 expression. Conclusion: Comparison between Herceptin responses obtained with Oncogramme and HER2 status of breast tumour cells confirmed that Oncogramme is a reliable method for prediction of patient cell sensitivity to anticancer drugs.

Leroy J.,CNRS XLIM Research Institute, Limoges | Hjeij F.,CNRS XLIM Research Institute, Limoges | Dalmay C.,CNRS XLIM Research Institute, Limoges | Melin C.,University of Limoges | And 7 more authors.
2015 IEEE MTT-S International Microwave Symposium, IMS 2015 | Year: 2015

This paper deals with the development and characterization of a high frequency (HF) label-free microfluidic biosensor for the non-invasive analysis of cell intracellular properties. The presented microfluidic biosensor is based on a band pass filter architecture made of thick gold electrodes designed to ensure a high sensitivity to cells flowing in the microfluidic channel. In a first step, to prove the feasibility of the proposed approach, HF measurements have been successfully achieved on polystyrene beads. Then, combining HF measurements with dielectrophoresis forces, to trap cells in the sensitive area, it has been possible to characterize cell dielectric properties without any denaturation. We demonstrate here the proof of concept of using high frequency impedance spectroscopy to analyze single cells in a microfluidic environment. © 2015 IEEE.

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