PubMed | Oncology and Research Center
Type: Journal Article | Journal: Journal of clinical oncology : official journal of the American Society of Clinical Oncology | Year: 2016
6571 Background: Recently, patients with acute promyelocytic leukemia (APL) have experienced significant clinical gains after treatment with arsenic trioxide (AsFrom 95 patients with APL, 85 patients who achieved complete remission (CR) were sequentially evaluated during 4-60 months period of follow-up by conventional RT-PCR. A total of 30 patients (6 relapsed and 24 in continued long remission) were selected and monitored by quantitative real-time PCR (RQ-PCR) assay. Using Hybridization Probes technology, the expression of PML-RAR/G6PDH transcript ratio was analyzed in serial PB samples taken at different courses of disease and the results were compared with the clinical outcome.More than 90% of patients obtained molecular remission, as determined by conventional RT-PCR in 1-3 months after start of arsenic therapy. RQ-PCR analyses showed a rapid rate of clearance of PML-RAR/G6PDH transcript level during the courses of arsenic therapy. In majority of the patients in CR the level of PML-RAR/ G6PDH ratio was always below 510Using a sensitive and quantitative method provided valuable information about effectiveness of arsenic as a front-line therapy in the management of newly diagnosed APL. Our study highlights the usefulness of PB and the definition of threshold level for early prediction of relapse. The threshold level correlates well with relapse risk; therefore, transcript ratio below the level should be regarded as a goal in the clinical management of this disease. No significant financial relationships to disclose.