Polosukhin V.V.,Vanderbilt University |
Richmond B.W.,Vanderbilt University |
Du R.-H.,Vanderbilt University |
Cates J.M.,Vanderbilt University |
And 8 more authors.
American Journal of Respiratory and Critical Care Medicine | Year: 2017
Rationale: Maintenance of a surface immune barrier is important for homeostasis in organs with mucosal surfaces that interface with the external environment; however, the role of the mucosal immune system in chronic lung diseases is incompletely understood. Objectives: We examined the relationship between secretory IgA (SIgA) on the mucosal surface of small airways and parameters of inflammation and airway wall remodeling in chronic obstructive pulmonary disease (COPD). Methods: We studied 1,104 small airways (<2 mm in diameter) from 50 former smokers with COPD and 39 control subjects. Small airways were identified on serial tissue sections and examined for epithelial morphology, SIgA, bacterial DNA, nuclear factor-kB activation, neutrophil and macrophage infiltration, and airway wall thickness. Measurements and Main Results: Morphometric evaluation of small airways revealed increased mean airway wall thickness and inflammatory cell counts in lungs from patients with COPD compared with control subjects, whereas SIgA level on the mucosal surface was decreased. However, when small airways were classified as SIgA intact or SIgA deficient, we found that pathologic changes were localized almost exclusively to SIgA-deficient airways, regardless of study group. SIgA-deficient airways were characterized by (1) abnormal epithelial morphology, (2) invasion of bacteria across the apical epithelial barrier, (3) nuclear factor-kB activation, (4) accumulation of macrophages and neutrophils, and (5) fibrotic remodeling of the airway wall. Conclusions: Our findings support the concept that localized, acquired SIgA deficiency in individual small airways of patients with COPD allows colonizing bacteria to cross the epithelial barrier and drive persistent inflammation and airway wall remodeling, even after smoking cessation. Copyright © 2017 by the American Thoracic Society.
Bel'skaya L.V.,Omsk State Technical University |
Kosenok V.K.,Omsk State Medical Academy |
Sarf E.A.,Skolkovo Innovation Center
Archives of Oral Biology | Year: 2017
Background The high rate of changes in the composition of saliva can be used for the monitoring of various biorhythms in order to study the physiological characteristics of the human body. Research objective The study of the dynamics of the near-24-h mineral composition of saliva in men and women. Methods The study involved 20 men and 20 women, age 23.1 ± 0.9 years. Saliva samples were collected every 3 h during the day within 10 min. The mineral composition of saliva was defined by the capillary electrophoresis technique. The cross-group differences were evaluated using the non-parametric criterion. Results It was shown that the maximum values of the mineralizing capacity fall to 7–8 a.m. and 18–19p.m., which is due to the change in the rate of saliva secretion. The near-24-h dynamics of the saliva pH practically coincides with the dynamics of the Ca/P ratio; there are evident maxima at 9:00 am and 15–18p.m. The values of the Na/K ratio are out of phase with the Ca/P ratio. There is one maximum, corresponding to 3 am in the night, and one minimum at 12–13p.m., which is due to a decreased level of sodium and increased potassium concentration. Statistically valid differences between men and women in pH (p < 0.001), concentrations of inorganic phosphorus (p < 0.001), as well as Ca/P coefficient (p = 0.011) were identified. Conclusion The dynamics of the studied parameters during 24 h is characterized by pronounced intervals. © 2017 Elsevier Ltd
Gorbunova I.L.,Omsk State Medical Academy |
Mikheikina N.I.,Omsk State Medical Academy
Stomatologiia | Year: 2016
The paper presents the rationale to dental caries prevention concerning fluoride varnish optimal concentration, method of application and exposure on dental enamel of caries-resistant and caries liable persons. It was found that enamel-sealing liquid applicated on dental enamel for three minutes with рН 7,0 demonstrated the best preventive effect.Обоснован выбор кариеспрофилактического средства, а также определены его оптимальная концентрация, способ нанесения и экспозиция на зубную эмаль кариесрезистентных и кариесподверженных лиц. Наилучший кариеспрофилактический эффект демонстрирует эмальгерметизируюший ликвид, апплицируемый на зубную эмаль на 3 мин и имеющий рН 7,0.
Polosukhin V.V.,Vanderbilt University |
Cates J.M.,Vanderbilt University |
Lawson W.E.,Vanderbilt University |
Zaynagetdinov R.,Vanderbilt University |
And 11 more authors.
American Journal of Respiratory and Critical Care Medicine | Year: 2011
Rationale: Although airway inflammation can persist for years after smoking cessation in patients with chronic obstructive pulmonary disease (COPD), the mechanisms of persistent inflammation are largely unknown. Objectives: We investigated relationships between bronchial epithelial remodeling, polymeric immunoglobulin receptor (pIgR) expression, secretory IgA (SIgA), airway inflammation, and mural remodeling in COPD. Methods: Lung tissue specimens and bronchoalveolar lavage were obtained from lifetime nonsmokers and former smokers with or without COPD. Epithelial structural changes were quantified by morphometric analysis. Expression of pIgR was determined by immunostaining and real-time polymerase chain reaction. Immunohistochemistry was performed for IgA, CD4 and CD8 lymphocytes, and cytomegalovirus and Epstein-Barr virus antigens. Total IgA and SIgA were measured by ELISA and IgA transcytosis was studied using cultured human bronchial epithelial cells. Measurements and Main Results: Areas of bronchial mucosa covered by normal pseudostratified ciliated epithelium were characterized by pIgR expression with SIgA present on the mucosal surface. In contrast, areas of bronchial epithelial remodeling had reduced pIgR expression, localized SIgA deficiency, and increased CD4+ and CD8+ lymphocyte infiltration. In small airways (<2 mm), these changes were associated with presence of herpesvirus antigens, airway wall remodeling, and airflow limitation in patients with COPD. Patients with COPD had reduced SIgA in bronchoalveolar lavage. Air-liquid interface epithelial cell cultures revealed that complete epithelial differentiation was required for normal pIgR expression and IgA transcytosis. Conclusions: Our findings indicate that epithelial structural abnormalities lead to localized SIgA deficiency in COPD airways. Impaired mucosal immunity may contribute to persistent airway inflammation and progressive airway remodeling in COPD.
Osipenko M.F.,Novosibirsk State Medical University |
Livzan M.A.,Omsk State Medical Academy |
Bikbulatova E.A.,Novosibirsk State Medical University
Terapevticheskii Arkhiv | Year: 2014
Aim. To improve the quality of eradication therapy (ET), by increasing patient compliance. Subjects and methods. An open-label cohort multicenter randomized study was conducted. A total of 350 patients with Helicobacter pylori infection-associated diseases and indications for ET were examined. Work was done to improve compliance in a study patient group before treatment. Results: In the study group of patients motivated for treatment, the efficiency of ET was significantly higher than in the control group. The factors influencing treatment adherence were identified and methods to optimize compliance during H. pylori eradication were developed. Conclusion. Awareness of the essence of the disease in a patient and his higher motivation for compliance are one of the components of successful H. pylori eradication.
Osipenko M.F.,Novosibirsk State University |
Livzan M.A.,Omsk State Medical Academy |
Skalinskaya M.I.,Novosibirsk State University |
Lyalyukova E.A.,Omsk State Medical Academy
Terapevticheskii Arkhiv | Year: 2015
Aim. To estimate the informative value of a noninvasive fecal (calprotectin) test in the differential diagnosis of bowel diseases. Subjects and methods. One hundred and forty-two patients who had visited their physicians with intestinal disorders (a change in stool frequency and patterns, abdominal pain) were examined in an open-label multicenter cross-sectional study. The examinees' mean age was 35±2.43 years. Gender- and age-matched comparison groups consisted of healthy individuals and patients with irritable bowel syndrome. Fecal calprotectin (FCP) was determined using a BUHLMANN Calprotectin ELISA kit. All the patients underwent colonoscopy. Results. In the patients with organic bowel diseases (ulcerative colitis, Crohn's disease, lymphocytic colitis, colonic diverticular disease, colonic adenomas, celiac disease), the concentration of FCP was found to be substantially higher than that in the healthy individuals and patients with irritable bowel syndrome. The highest calprotectin levels were noted in the patients with inflammatory bowel diseases. Conclusion. This study demonstrated the high diagnostic value of the calprotectin test for the differential diagnosis of organic and functional bowel diseases.
Akhmedov V.A.,Omsk State Medical Academy
Patologicheskaia fiziologiia i èksperimental'naia terapiia | Year: 2013
The matrix metalloproteases are the big family of endopeptidases that takes part in degradation of extracellular matrix. The investigation of clinical significance of matrix metalloproteases in heart and vascular pathology can organize the pathogenetic treatment of the patients with heart and vascular diseases.
Djurjagin N.M.,Omsk State Medical Academy
Biophysics (Russian Federation) | Year: 2012
Biological models of osseous tissue and periosteum have been developed. Biocompatible materials of titaniumnickel as an integral part of implant-tissue composites have been already functioning for five years, substituting for damaged bone fragments. The defects of skeletal kinematic sections of the mandible of experimental animals were replaced by biomechanical models of implant-tissue composites of the osseous tissue and periosteum. The composites were formed by joining the models of extracellular matrixes of osseous tissue and periosteum and the natural tissues. The validity of methodological propositions and optimal technological solutions have been proved by the absence of life-time signs of biochemical, biomechanical, hysteresis or thermodynamic incompatibility on tissue and skeletal levels. © 2012 Pleiades Publishing, Ltd.
Navrotsky A.N.,Omsk State Medical Academy
Terapevticheskii Arkhiv | Year: 2014
The paper analyzes the clinical and laboratory manifestations of acute Opisthorchis invasion concurrent with chronic hereditary hemolytic anemia before and after antihelminthic therapy. It gives the results of direct clinical observation of a patient in the acute phase of opisthorchiasis in the presence of Minkowsky-Shauffard disease. His examination encompassed conventional laboratory and instrumental studies used in hepatology, such as physical, biochemical, and immunological examinations (tests for viral hepatitis markers and autoimmune liver diseases), and abdominal ultrasound scanning and magnetic resonance imaging. The patient with acute opisthorchiasis concurrent with hemolytic anemia was found to have a preponderance of clinical and laboratory manifestations of hepatocholangitis in the early stages of the disease and a prevalence of subfebrility with progressive eosinophilia in the presence of regressive symptoms. The clinical and laboratory signs of hereditary microspherocytosis suggest that the process is decompensated. The found clinical and laboratory changes correspond to the natural course of the diseases. The magnitude of changes in laboratory parameters suggests that there is an intercurrent interaction of infectious and somatic diseases, but does not hinder dehelminthization.
Tso Ukanov Y.T.,Omsk State Medical Academy |
Tso Ukanov A.Y.,Omsk State Medical Academy |
Nikolaychuk A.,Omsk State Medical Academy
Phlebolymphology | Year: 2015
Aim: the study was aimed at investigating the frequently encountered clinical group of patients presenting with subjective leg symptoms without visible signs of chronic venous disorders. The great saphenous vein (GSV) of such patients was investigated using duplex scanning (DS) to verify whether a reflux could occur in certain circumstances, ie, at the end of the day in an orthostatic position. If there was a reflux, the possibility to eliminate it with a drug treatment, ie, MPFF, was assessed. Material and methods: women consulting for complaints related to chronic venous disorders (CVDs) of the lower extremities, but without visible signs; therefore, with a C0s, En, An, Pn classification according to the Clinical, Etiological, Anatomical, Pathophysiological (CEAP) classification, were enrolled. Symptoms were assessed using a 10 cm visual analogue scale (VAS) and the patients’ quality of life was assessed using the ChronIc Venous Insufficiency Quality of life questionnaire-20 items (CIVIQ-20). Patients underwent a DS of the lower extremities in the upright position twice a day: once in the morning (before 10 am) and once in the evening after normal physical efforts (after 6 pm). The investigations included the following measurements: (i) reflux duration; (ii) GSV diameter in the groin area (mm); and (iii) difference in the GSV diameter between the evening and morning values (mm). Patients with evening reflux received a 2-month pharmacological treatment with MPFF (1000 mg of MPFF once a day in the morning). A DS investigation was repeated after 2 months of treatment in these patients. Results: a total of 41 C0s women aged 21 to 57 years (mean age, 35.4±15.1 years) were enrolled in the study and then investigated with DS. A total of 15 of the patients had no reflux, either in the morning or in the evening. The remaining 26 patients had no reflux in the morning, but presented with an evening GSV reflux. As for reflux extent, 2 had an axial reflux and 24 had a segmental reflux, of which 11 were proximal and 13 were medial. Regarding reflux type, 4 had an intervalvular reflux and 22 had a commissural reflux. The evening GSV diameter in the subgroup with reflux (n=26) was significantly larger (P<0.05), compared with patients without evening reflux (n=15; 6.33 mm [95% confidence interval (CI), 4.50- 8.00 mm] vs 5.45 mm [95% CI, 4.00-6.50 mm]). This was also true for the difference in GSV diameter between the evening and morning (0.82 mm [95% CI, 0.30-1.20 mm] vs 0.42 mm [95% CI, 0.10-0.65 mm]). Of the 26 patients with previous baseline evening GSV reflux who were investigated after a 2-month MPFF treatment, 22 no longer had reflux at 6pm, and 4 had a nonsignificantly reduced length of reflux. In parallel, the GSV diameter decreased from 6.33 mm (95% CI, 4.50-8.00 mm) to 5.50 mm (95% CI, 1.10- 7.00 mm), and the difference in GSV diameter between the evening and morning decreased from 0.82 mm (95% CI, 0.30-1.20 mm) to 0.37 mm (95% CI, 0.10-0.70 mm; P=0.000008). There was a parallel significant decrease in the intensity of subjective symptoms as demonstrated by the VAS score and a significant improvement in the patients’ quality of life after treatment (P=0.00001 for both). Conclusion. C0s, En, An, Pn patients may present with a transient reflux in the GSV that occurs at the end of day. Objective abnormalities can be detected by DS investigations and by measuring the difference in GSV diameter between the morning and the evening. Treatment with MPFF (1000 mg of MPFF once a day in the morning for 2 months) resulted in the elimination of the evening GSV reflux in most of the treated patients, decrease in vein diameter and also resulted in beneficial effects on symptom relief and quality of life improvement. © 2015, LLS SAS. All rights reserved.