Rocheleau M.-J.,Omega Laboratories Ltd. |
Rocheleau M.-J.,PARC Inc. |
Larouche E.,Omega Laboratories Ltd. |
Salamu C.,Omega Laboratories Ltd. |
Curca M.,Omega Laboratories Ltd.
Journal of Pharmaceutical Analysis | Year: 2012
Liquid chromatography (LC) is considered by many as a mature technique. Nonetheless, LC technology continues to evolve driven by the need for high-throughput and high-resolution analyses. Over the past several years, small particle size packing materials have been introduced by several column manufacturers to enable fast and efficient LC separations. Several examples of pharmaceutical analyses, including impurity profiling of taxanes and atracurium besylate, in-process testing of peptides in injectable dosage form, using sub-2 μm column technology are presented in this paper, demonstrating some of the capabilities and limitations of the technology. © 2012 Xian Jiaotong University.
Wyman J.F.,Cuyahoga County Regional Forensic Science Laboratory |
Lavins E.S.,Cuyahoga County Regional Forensic Science Laboratory |
Engelhart D.,Omega Laboratories Inc |
Armstrong E.J.,Cuyahoga County Regional Forensic Science Laboratory |
And 5 more authors.
Journal of Analytical Toxicology | Year: 2013
3,4-Methylenedioxypyrovalerone (MDPV) is a psychoactive, synthetic analog of the central nervous system stimulant cathinone. Its recent popularity as a recreational drug in the United States has led to numerous reports to poison control centers across the country. As with other synthetic cathinones, the recreational use of MDPV has resulted in death. MDPV is thought to exert its pharmacologic effects by inhibiting the reuptake of dopamine and norepinephrine. This report describes the case of an exposure of a 39-year-old male to MDPV, which resulted in his death. Postmortem concentrations of MDPV in various tissues were measured. The detection of MDPV in tissues and fluids was accomplished using gas chromatographymass spectrometry analysis after solid-phase extraction. Blood analysis also demonstrated therapeutic levels of lamotrigine, fluoxetine, risperidone, benztropine, pseudoephedrine and ibuprofen. The detection of cathinones in hair was conducted using high-performance liquid chromatographytandem mass spectrometry after solid-phase extraction. MDPV was uniformly distributed among multiple tissues (blood, brain, muscle, cerebrospinal fluid and lung) at concentrations of approximately 0.4 to 0.6 μg/mL. Tissue and fluids responsible for detoxification/excretion had higher concentrations of MDPV (kidney, liver and bile >0.8 μg/mL). A blood concentration >0.4 μg/mL was judged sufficient to cause death. The cause of death was ruled MDPV intoxication resulting in cardiac arrhythmia.