Al Qurayyāt, Saudi Arabia
Al Qurayyāt, Saudi Arabia

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Hechmi M.,Olive Research Unit | Khaled M.,Olive Research Unit | Echarari F.,Olive Research Unit
American Journal of Plant Physiology | Year: 2015

This work aimed to optimize the pollen germination of four olive cultivars Koroneiki, Frantoio, Manzanille and Nabali, in order to use pollen resource in cross pollination assays. Pollen samples were subjected to 2 experiments to assess the effect of boric acid and storage treatments on the germination fertility (viability, germination and tube growth). First, pollen of each cultivar was exposed to boric acid at 0 and 100 ppm added to an agarized medium. For storage, pollen grains were conserved in freezer (-20°C), in refrigerator (+10°C) and at room temperature (25°C; control) during 0, 1, 6 and 12 months. The results showed that the addition of 100 ppm boric acid to the culture medium not only increased the pollen germination by 12-24.9% according to the cultivars but also resulted in an increased tube length after 5 h instead of 9 h in control (without boric acid). Pollen of all cultivars showed greater tolerance for low temperature storage -20 and 10°C and not for high temperature (25°C). Increasing storage period of pollen grains from 1 to 12 months, resulted in a decrease in those p arameters in all cultivars tested, these reductions were evident after one month of storage. Pollen death occurred rapidly after 1 month when held at room temperature (25°C) while for temperatures storage of -20 and +10°C, pollen death occurred after 6 months. These pollen characters indicate a potential for using these pollen grains as pollinator for self-incompatible olive cultivars found in Al-Jouf conditions. © 2015 Academic Journals Inc.


Mehri H.,Olive Research Unit | Mhanna K.,Olive Research Unit | Soltane A.,Olive Research Unit
American Journal of Plant Physiology | Year: 2013

'Arbequina' olive cultivar which is the most grown for high quality olive oil in Spain, has the potential for being cultured in KSA. The aim of this study was to investigate the efficiency of inorganic and organic growing substrates on the root growth and the root morphology of Arbequina olive cuttings. One-year-old cuttings were dipped in IBA 4000 ppm before planting in 3 substrates: sand, perlite (inorganic) and peat moss (organic). The results showed that IBA (1H-indole-3-butyric acid) at 4000 ppm combined with perlite substrate were successful in promoting earlier root development and limited callus formation in the early period of rooting. Cuttings rooted more profusely on perlite substrate and developed significantly more and longer roots per cutting. Also best survival and easily acclimatization of plants in greenhouse was unregistered. On the contrary, peat moss developed a root system with high callus and proved to be less qualitative with less number and shorter roots per cutting, suggesting that peat moss was not a suitable environment for adventitious root formation of Arbequina cultivar. Good callus formation was also obtained with untreated control cuttings where late emergence was observed. Roots of cuttings necrosed and died by the end of growing period. Considering all studied aspects of root system, the physical properties of substrates should be carefully choose as rooting medium of olive cuttings. © 2013 Academic Journals Inc.


Mehri H.,Olive Research Unit | Soltane A.,Olive Research Unit | Richene F.C.,Olive Research Unit | Mhanna K.,Olive Research Unit
American Journal of Plant Physiology | Year: 2013

During the last 15 years, many olive cultivars have been introduced in the Kingdom of Saudi Arabia without any study on their biological and physiological behaviour and characteristics. This work aimed to determine the floral biology and the reproductive behaviour of five olive cultivars planted in Al-Jouf region and to choose the right cultivars adapted to the agro-ecological conditions of KSA. For each cultivar, floral biology in relation to phenological stages and flower quality and fertility behaviour under self and free pollination were determined for each cultivar. Notable significant differences between the cultivars were observed in all characteristics considered. Among the productive characters, all the olive cultivars showed a good productive behaviour with particular interest for 'Picual' and 'Sourani' cultivars. Regarding pollen characteristics, 'Sourani' and 'Picholine' reflected better pollen quality while the evaluation of flower quality and bloom showed good performance in all cultivars except for 'Zaiti' and 'Nabali' cultivars. In all cultivars, a positive response to open pollination was significantly consistent when compared with self pollination. The self-incompatibility index ISI was higher than 0.5 for all cultivars classified them as partially self incompatible cultivars. These results suggest that the five olive cultivars performed well in Al-Jouf conditions with a high phenological variability between cultivars for the parameters evaluated. © 2013 Academic Journals Inc.

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