News Article | April 24, 2017
The Drum Search Awards are an event in the calendar a Search Marketing agency never wants to miss. Entries to the search awards have reportedly been higher than ever this year, with iThinkMedia in the final with some of the leading agencies in the UK. Entering for the first time, iThinkMedia have been nominated by this national award team for ‘Organic Team of the Year’ and ‘Biddable Team of the Year’. For both awards iThinkMedia have proven agency growth, innovation, successful campaigns and a high talent across SEO, PPC and content. For a small agency of 25 people, this is a huge step to getting Hertfordshire on the map within the wider Search Marketing industry. The Drum Search Awards finals are due to take place at a black-tie ceremony in the Marriott Hotel in Grosvenor Square, London, on the 27th May 2017 Chris Ailey, Founder of iThinkMedia said: “To be nominated for two Drum awards at the first time of asking is a fantastic achievement. I’m very proud of my team; they have shown that by following our ethos of hard work, innovative thinking and high quality service, it really does pay off. It’s a truly exciting time to be working with iThinkMedia.“ Syed Ali, Head of Search for iThinkMedia said: “The Drum Search Awards are recognising the very best work in the search industry, by awarding innovation, creativity and talent. Being a finalist really shows the high quality of work we have delivered consistently over the last few years here at iThinkMedia. It’s a great achievement and a testament to our on-going and collective desire to improve and evolve." Dominic Corr, PPC Manager for iThinkMedia said: “The Biddable Team of the Year nomination cap's off what has been a fantastic year for us as a department. A huge amount of credit must go to the team for the results and achievements they’ve made along the way but also to our clients for sharing this journey with us." The Drum Search Awards celebrate the individuals and companies at the forefront of the search Industry. Described by judges as 'the one to win' these awards provide the perfect opportunity for those entering to prove they are the best at what they do. iThinkMedia are a specialist search engine optimisation agency based in rural Hertford just outside London iThinkMedia is a digital marketing agency founded by Chris Ailey in 2008 and based in an 18th Century barn in idyllic Hertfordshire countryside. Growing to 25 people in 2017, iThinkMedia are renowned for their expert digital marketing expertise and are well regarded in the industry due to their principled, transparent approach to search engine marketing. Working in synergy, they offer SEO, PPC and content services to a range of businesses mixed in size and industry. Read more at http://www.ithinkmedia.co.uk ● The Drum Search Awards for information on awards and nominees: https://www.thedrumsearchawards.com/ For more details, please contact our iThinkMedia team directly: Located at: iThinkMedia, The Old Barn, 2 Cole Green, Hertford, SG14 2NN.
Kirkland D.,Kirkland Consulting |
Reeve L.,Covance |
Gatehouse D.,Old Barn |
Vanparys P.,ALTOXICON BVBA
Mutation Research - Genetic Toxicology and Environmental Mutagenesis | Year: 2011
In vitro genotoxicity testing needs to include tests in both bacterial and mammalian cells, and be able to detect gene mutations, chromosomal damage and aneuploidy. This may be achieved by a combination of the Ames test (detects gene mutations) and the in vitro micronucleus test (MNvit), since the latter detects both chromosomal aberrations and aneuploidy. In this paper we therefore present an analysis of an existing database of rodent carcinogens and a new database of in vivo genotoxins in terms of the in vitro genotoxicity tests needed to detect their in vivo activity. Published in vitro data from at least one test system (most were from the Ames test) were available for 557 carcinogens and 405 in vivo genotoxins. Because there are fewer publications on the MNvit than for other mammalian cell tests, and because the concordance between the MNvit and the in vitro chromosomal aberration (CAvit) test is so high for clastogenic activity, positive results in the CAvit test were taken as indicative of a positive result in the MNvit where there were no, or only inadequate data for the latter. Also, because Hprt and Tk loci both detect gene-mutation activity, a positive Hprt test was taken as indicative of a mouse-lymphoma Tk assay (MLA)-positive, where there were no data for the latter. Almost all of the 962 rodent carcinogens and in vivo genotoxins were detected by an in vitro battery comprising Ames. +. MNvit. An additional 11 carcinogens and six in vivo genotoxins would apparently be detected by the MLA, but many of these had not been tested in the MNvit or CAvit tests. Only four chemicals emerge as potentially being more readily detected in MLA than in Ames. +. MNvit - benzyl acetate, toluene, morphine and thiabendazole - and none of these are convincing cases to argue for the inclusion of the MLA in addition to Ames. +. MNvit. Thus, there is no convincing evidence that any genotoxic rodent carcinogens or in vivo genotoxins would remain undetected in an in vitro test battery consisting of Ames. +. MNvit. © 2011 Elsevier B.V.
Gatehouse D.,Old Barn
Methods in Molecular Biology | Year: 2012
The most widely used assays for detecting chemically induced gene mutations are those employing bacteria. The plate incorporation assay using various Salmonella typhimurium LT2 and E. coli WP2 strains is a short-term bacterial reverse mutation assay specifically designed to detect a wide range of chemical substances capable of causing DNA damage leading to gene mutations. The test is used worldwide as an initial screen to determine the mutagenic potential of new chemicals and drugs. The test uses several strains of S. typhimurium which carry different mutations in various genes of the histidine operon, and E. coli which carry the same AT base pair at the critical mutation site within the trpE gene. These mutations act as hot spots for mutagens that cause DNA damage via different mechanisms. When these auxotrophic bacterial strains are grown on a minimal media agar plates containing a trace of the required amino-acid (histidine or tryptophan), only those bacteria that revert to amino-acid independence (His + or Tryp +) will grow to form visible colonies. The number of spontaneously induced revertant colonies per plate is relatively constant. However, when a mutagen is added to the plate, the number of revertant colonies per plate is increased, usually in a dose-related manner. This chapter provides detailed procedures for performing the test in the presence and absence of a metabolic activation system (S9-mix), including advice on specific assay variations and any technical problems. © 2012 Springer Science+Business Media, LLC.
Kirkland D.,Kirkland Consulting |
Gatehouse D.,Old Barn
Food and Chemical Toxicology | Year: 2015
Aspartame is a methyl ester of a dipeptide of aspartic acid and phenylalanine. It is 200× sweeter than sucrose and is approved for use in food products in more than 90 countries around the world. Aspartame has been evaluated for genotoxic effects in microbial, cell culture and animal models, and has been subjected to a number of carcinogenicity studies. The in vitro and in vivo genotoxicity data available on aspartame are considered sufficient for a thorough evaluation. There is no evidence of induction of gene mutations in a series of bacterial mutation tests. There is some evidence of induction of chromosomal damage in vitro, but this may be an indirect consequence of cytotoxicity. The weight of evidence from in vivo bone marrow micronucleus, chromosomal aberration and Comet assays is that aspartame is not genotoxic in somatic cells in vivo. The results of germ cell assays are difficult to evaluate considering limited data available and deviations from standard protocols. The available data therefore support the conclusions of the European Food Safety Authority (EFSA) that aspartame is non-genotoxic. © 2015 .
PubMed | Kirkland Consulting and Old Barn
Type: | Journal: Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association | Year: 2015
Aspartame is a methyl ester of a dipeptide of aspartic acid and phenylalanine. It is 200 sweeter than sucrose and is approved for use in food products in more than 90 countries around the world. Aspartame has been evaluated for genotoxic effects in microbial, cell culture and animal models, and has been subjected to a number of carcinogenicity studies. The in vitro and in vivo genotoxicity data available on aspartame are considered sufficient for a thorough evaluation. There is no evidence of induction of gene mutations in a series of bacterial mutation tests. There is some evidence of induction of chromosomal damage in vitro, but this may be an indirect consequence of cytotoxicity. The weight of evidence from in vivo bone marrow micronucleus, chromosomal aberration and Comet assays is that aspartame is not genotoxic in somatic cells in vivo. The results of germ cell assays are difficult to evaluate considering limited data available and deviations from standard protocols. The available data therefore support the conclusions of the European Food Safety Authority (EFSA) that aspartame is non-genotoxic.
News Article | October 27, 2016
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