Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital

Tokyo, Japan

Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital

Tokyo, Japan
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Kono M.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Hasegawa J.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Hasegawa J.,Tokyo Women's Medical University | Wakai S.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | And 8 more authors.
Transplantation Proceedings | Year: 2017

Background Sarcoidosis is a chronic systemic disease that is characterized by the formation of noncaseating granuloma and whose etiology is unclear. It is unclear whether patients with sarcoidosis are suitable organ donors. Case We treated a 56-year-old woman with pulmonary sarcoidosis who donated her kidney. She was previously in good health and was diagnosed with pulmonary sarcoidosis during her preoperative examination. Because she presented with no symptoms and was otherwise in good condition, donor nephrectomy was performed. Results Baseline biopsy examination showed no evidence of sarcoidosis. One year after transplantation, both the donor and the recipient had not developed kidney dysfunction or recurrence of sarcoidosis. Conclusion This is a rare case in which a patient with pulmonary sarcoidosis donated a kidney for transplantation, and both the recipient and the donor were clinically healthy. A patient with sarcoidosis and no kidney lesion can donate a living kidney, because transplantation appears to be safe for both the recipient and the donor. © 2017 Elsevier Inc.


Koda H.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Kimura Y.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Ishige I.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Eishi Y.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | And 2 more authors.
Acta oto-laryngologica | Year: 2010

CONCLUSION: We could represent the first quantitative analysis of the mutation rate at the cellular level in human inner ear of a patient with MELAS (mitochondrial encephalopathy, lactic acidosis and stroke-like episodes) by combining laser capture microdissection (LCM) and quantitative real time PCR.OBJECTIVES: We previously reported combining LCM and PCR to isolate mtDNA from the cells of specific tissues within a human archival celloidin-embedded temporal bone section without known otological history. Using this method, we quantitatively analyzed the rate of mtDNA 3243A > G mutation in the inner ear of a MELAS patient, and examined the correlation of the mutation rate at the cellular level and their histopathological condition.METHODS: We extracted each inner ear organs using LCM from temporal bone sections of a MELAS patient, and studied the mutation rate, which was calculated as the ratio of the amount of mutant mtDNA to the total mtDNA.RESULTS: We found that the mtDNA mutation rate was high in spiral ganglion cells and the saccular macula, but was comparatively low in hair cells of the organ of Corti, the stria vascularis and the facial nerve. With the exception of the stria vascularis, there was a good correlation between the mutation rate and the histological findings.


Koda H.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Kimura Y.,Tokyo Metropolitan Geriatric Medical Hospital | Ishige I.,Tokyo Medical and Dental University | Eishi Y.,Tokyo Medical and Dental University | And 2 more authors.
Acta Oto-Laryngologica | Year: 2010

Conclusion: We could represent the first quantitative analysis of the mutation rate at the cellular level in human inner ear of a patient with MELAS (mitochondrial encephalopathy, lactic acidosis and stroke-like episodes) by combining laser capture microdissection (LCM) and quantitative real time PCR. Objectives: We previously reported combining LCM and PCR to isolate mtDNA from the cells of specific tissues within a human archival celloidin-embedded temporal bone section without known otological history. Using this method, we quantitatively analyzed the rate of mtDNA 3243A >G mutation in the inner ear of a MELAS patient, and examined the correlation of the mutation rate at the cellular level and their histopathological condition. Methods: We extracted each inner ear organs using LCM from temporal bone sections of a MELAS patient, and studied the mutation rate, which was calculated as the ratio of the amount of mutant mtDNA to the total mtDNA. Results: We found that the mtDNA mutation rate was high in spiral ganglion cells and the saccular macula, but was comparatively low in hair cells of the organ of Corti, the stria vascularis and the facial nerve. With the exception of the stria vascularis, there was a good correlation between the mutation rate and the histological findings. © 2010 Informa UK Ltd. (Informa Healthcare, Taylor & Francis As).


Koda H.,Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital | Makino N.,Ooimachi ENT | Takahashi M.,Tokyo Medical and Dental University | Kurata N.,Tokyo Medical and Dental University
Journal of Otolaryngology of Japan | Year: 2014

A 62-year-old female visited us complaining of lacrimination and the swelling of the left side of the nasal dorsum. Granulation tissues were seen on the anterior-lateral wall of the left nasal cavity, and an imaging study revealed a mass occupying both the left nasal cavity and the anterior ethmoid sinuses invading the face with erosion of the nasal bone. Histological examination showed an epithelioid granuloma without caseous necrosis which mostly suggested sarcoidosis. The affected lesions became larger 11 months after the initial examination, and a biopsy was performed again, which revealed the same pathological findings. Although the biopsy specimen was negative based on the PCR findings, tubercle bacillus was detected in the culture 7 weeks later. The patient was finally diagnosed as having primary tuberculosis of the nose and paranasal sinuses because systemic examination showed no evidence of tuberculosis in other areas. She was treated with antituberculosis medication, and the affected nasal lesions disappeared. It is important to perform a biopsy and tissue culture repeatedly when we encounter an intractable and undiagnosed disease in the nasal cavity.


PubMed | Tokyo Metropolitan Health and Medical Treatment Corporation Okubo Hospital
Type: Journal Article | Journal: Acta oto-laryngologica | Year: 2010

We could represent the first quantitative analysis of the mutation rate at the cellular level in human inner ear of a patient with MELAS (mitochondrial encephalopathy, lactic acidosis and stroke-like episodes) by combining laser capture microdissection (LCM) and quantitative real time PCR.We previously reported combining LCM and PCR to isolate mtDNA from the cells of specific tissues within a human archival celloidin-embedded temporal bone section without known otological history. Using this method, we quantitatively analyzed the rate of mtDNA 3243A > G mutation in the inner ear of a MELAS patient, and examined the correlation of the mutation rate at the cellular level and their histopathological condition.We extracted each inner ear organs using LCM from temporal bone sections of a MELAS patient, and studied the mutation rate, which was calculated as the ratio of the amount of mutant mtDNA to the total mtDNA.We found that the mtDNA mutation rate was high in spiral ganglion cells and the saccular macula, but was comparatively low in hair cells of the organ of Corti, the stria vascularis and the facial nerve. With the exception of the stria vascularis, there was a good correlation between the mutation rate and the histological findings.

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