Okinawa Industrial Technology Center

Uruma, Japan

Okinawa Industrial Technology Center

Uruma, Japan

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Saito H.,Japan National Research Institute of Fisheries Science | Xue C.,Ocean University of China | Yamashiro R.,Okinawa Industrial Technology Center | Moromizato S.,Okinawa Local Government | Itabashi Y.,Hokkaido University
Journal of Phycology | Year: 2010

The lipid and fatty acid compositions in two edible subtropical algae (the brown alga Cladosiphon okamuranus Tokida and the green alga Caulerpa lentillifera J. Agardh) were determined to clarify their lipid characteristics and nutritional values. Glycolipids and phospholipids were the major lipid classes, with significant levels of triacylglycerols. Polyunsaturated fatty acids (PUFA) were the major fatty acids of both algae. The lipid class composition and major fatty acids were similar in both the algal species, irrespective of wild and cultured specimens. Typical n-6 PUFA, such as 18:2n-6 (linoleic acid) and 20:4n-6 (arachidonic acid), occurred in characteristically high levels in both of the algae. High levels of n-3 PUFA were measured in all lipid classes of both species without 22:6n-3 (docosahexaenoic acid), 18:3n-3, 18:4n-3, and 20:5n-3 (eicosapentaenoic acid) for Cl. okamuranus; and 16:3n-3, 18:3n-3, and 20:5n-3 for Ca. lentillifera. The finding suggests that the green algal species, which mainly biosynthesizes short-chain (C16 and C18) PUFA, differs from that of the brown alga, which is capable of biosynthesizing high 20:5n-3 levels. The PUFA levels in glycolipids of the two algal species comprised up to 60%, even though they are subtropical marine species. High n-6 PUFA levels in the algal lipids probably influence the significant levels of n-6 PUFA in herbivorous fishes, because the n-6 PUFA levels in marine fish lipids are generally undetectable or negligible. © 2010 Phycological Society of America.


Calabia B.P.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Okinawa Industrial Technology Center | Aiba S.,Japan National Institute of Advanced Industrial Science and Technology
Biotechnology Letters | Year: 2011

Of six strains of lactic acid-producing alkaliphilic microorganisms, Halolactibacillus halophilus was most efficient. It produced the highest concentration and yield of lactic acid, with minimal amounts of acetic and formic acid when sucrose and glucose were used as substrate. Mannose and xylose were poorly utilized. In batch fermentation at 30°C, pH 9 with 4 and 8% (w/v) sucrose, lactic acid was produced at 37.7 and 65.8 g l-1, with yields of 95 and 83%, respectively. Likewise, when 4 and 8% (w/v) glucose were used, 33.4 and 59.6 g lactic acid l-1 were produced with 85 and 76% yields, respectively. l-(+)-lactic acid had an optical purity of 98.8% (from sucrose) and 98.3% (from glucose). © 2011 Springer Science+Business Media B.V.


Ugwu C.U.,Okinawa Industrial Technology Center | Tokiwa Y.,Okinawa Industrial Technology Center | Ichiba T.,Okinawa Industrial Technology Center
Bioresource Technology | Year: 2011

Feasibility of producing (R)-3-hydroxybutyric acid ((R)-3-HB) using wild type Azohydromonas lata and its mutants (derived by UV mutation) was investigated. A. lata mutant (M5) produced 780. mg/l in the culture broth when sucrose was used as the carbon source. M5 was further studied in terms of its specificity with various bioconversion substrates for production of (R)-3-HB. (R)-3-HB concentration produced in the culture broth by M5 mutant was 2.7-fold higher than that of the wild type strain when sucrose (3% w/v) and (R,S)-1,3-butanediol (3% v/v) were used as carbon source and bioconversion substrate, respectively. Bioconversion of resting cells (M5) with glucose (1% v/w), ethylacetoacetate (2% v/v), and (R,S)-1,3-butanediol (3% v/v), resulted in (R)-3-HB concentrations of 6.5. g/l, 7.3. g/l and 8.7. g/l, respectively. © 2011 Elsevier Ltd.


Taira J.,Okinawa National College of Technology | Ohmine W.,Okinawa National College of Technology | Ogi T.,Okinawa Industrial Technology Center | Nanbu H.,University of Ryukyus | Ueda K.,University of Ryukyus
Bioorganic and Medicinal Chemistry Letters | Year: 2012

A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by 1H, 13C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-c -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-c treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging. © 2011 Elsevier Ltd. All rights reserved..


Ugwu C.U.,University of Tsukuba | Tokiwa Y.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Okinawa Industrial Technology Center | Aoyagi H.,University of Tsukuba
Journal of Polymers and the Environment | Year: 2012

The feasibility of utilizing non edible rice (broken rice) for production of fine materials such as poly(3-hydroxybutyrate) (PHB) was considered as one of the alternative ways of keeping the environment clean for sustainable development. Thus, production of PHB from broken rice by simultaneous saccharification and fermentation (SSF) was investigated. During the SSF process, the rice (15% w/v) material was hydrolyzed to glucose, which was utilized by Cupriavidus necator for growth and production of PHB. The PHB content reached 38% at 58 h fermentation. The PHB had weight average molar mass (Mw) and polydipersity index of 3. 82 × 10 5 (g/mol) and 4. 15, respectively. Differential calorimetric scan of the PHB showed a melting temperature (Tm) of 176 °C. Given that the PHB was a homopolymer (which consisted of (R)-3-hydroxybutyric acid monomers), it was thought that broken rice could be a raw material for production of both PHB and (R)-3-hydroxybutyric acid. This SSF process would not only help in the utilization of broken rice or non edible rice, but would also serve as a model for utilization of other raw materials that contain starch for production of PHB. © 2011 Springer Science+Business Media, LLC.


Rob T.,University of Ryukyus | Ogi T.,University of Ryukyus | Ogi T.,Okinawa Industrial Technology Center | Maarisit W.,University of Ryukyus | And 2 more authors.
Molecules | Year: 2011

Pentylphenols 1 and 2, cyclopropane fatty acid 3, and cyclopentenones 4 and 5, were isolated from an ascidian, Diplosoma sp. The structures of 1-5 were determined by spectroscopic analysis and/or synthesis. Compound 1 inhibited the division of fertilized sea urchin eggs and compound 4 showed mild cytotoxity against HCT116 cells (human colorectal cancer cell).© 2011 by the authors; licensee MDPI, Basel, Switzerland.


Taira J.,Okinawa National College of Technology | Tsuchida E.,Okinawa National College of Technology | Katoh M.C.,Okinawa National College of Technology | Uehara M.,Okinawa National College of Technology | Ogi T.,Okinawa Industrial Technology Center
Food Chemistry | Year: 2015

This study was designed to assess the antioxidant capacity of betacyanins as indole derived plant pigments, such as betanin, phyllocactin and betanidin. The antioxidant capacity of the betacyanins was evaluated as an index of radical scavenging ability using the peroxyl radical generating system in the presence of AAPH and NO generating system using NOR3 as an NO donor. The peroxyl radical scavenging capacity was dose-dependent in the low concentration range (25-100 nM). The mol-Trolox equivalent activity/mol compound (mol-TEA/mol-compound) as an index of the antioxidant capacity indicated the following order at 10.70 ± 0.01, 3.31 ± 0.14 and 2.83 ± 0.01 mol-TEA/mol-compound for betanidin, betanin and phyllocactin, respectively. In addition, betacyanins reduced the nitrite-level in the low concentration range of 2.5-20 μM. The IC50 values (μM) of nitrogen radical scavenging activity were 24.48, 17.51 and 6.81 for betanin, phyllocactin and betanidin. ESR studies provided evidence that the compounds directly scavenged NO. These results indicated that betacyanins have a strong antioxidant capacity, particularly betanidin with a catechol group had higher activity than those of the glycoside of betacyanins. This study demonstrated that the betacyanins will be useful as natural pigments to provide defence against oxidative stress. © 2014 Elsevier Ltd. All rights reserved.


Ogi T.,Okinawa Industrial Technology Center | Higa M.,Okinawa Industrial Technology Center | Maruyama S.,Japan National Institute of Advanced Industrial Science and Technology
Journal of Agricultural and Food Chemistry | Year: 2011

Tyrosinase, trypsin, and tryptase are known to play important roles in melanin production of human skin. This paper describes the study of the inhibitory effect of Balanophora fungosa on melanin. The 50% EtOH extract obtained from B. fungosa indicated an inhibitory effect on mushroom tyrosinase activity with an IC 50 value of 15 μg/mL. Bioassay-guided fractionation of the active extract resulted in the isolation of four known compounds. Their structures were identified as 1-O-(E)-caffeoyl-3-O-galloyl-4,6- (S)-HHDP-β-d-glucopyranose (1), 1-O-(E)-caffeoyl-3,4,6-tri-O-galloyl- β-d-glucopyranose (2), caffeoyl-β-d-glucopyranose (3), and abietin (4) on the basis of spectroscopic analyses and comparison of their spectral data with those in the literature. Compounds 1 and 2 prevented pigmentation of melanin in a three-dimensional cultured human skin model. Furthermore, compounds 1 and 2 indicated inhibitory activities against trypsin and tryptase. © 2011 American Chemical Society.


Maehira F.,University of Ryukyus | Motomura K.,University of Ryukyus | Ishimine N.,University of Ryukyus | Miyagi I.,University of Ryukyus | And 2 more authors.
Nutrition Research | Year: 2011

Silicon is rich in the normal human aorta but decreases with age and the development of atherosclerosis. We hypothesized that soluble silica (Si) and coral sand (CS), as a natural Si-containing material, would suppress high blood pressure (BP) in spontaneously hypertensive rats (SHRs), and clarify the observed antihypertensive mechanism by cell cultures by quantifying messenger RNA expressions in the aorta. In SHR fed diets containing 1% Ca supplemented with CaCO3 as the control (CT) and CS in a Ca-deficient diet and containing 50 mg/kg Si in the CT diet for 8 weeks, systolic BP was significantly (P < .05) lowered by 18 mm Hg for the Si group and 16 mm Hg for the CS group compared with the control CT group with 207 mm Hg. Magnesium (Mg) uptake by rat aortic smooth muscle cells significantly increased (177%, P < .005) in cells cultured with a physiologic Mg level plus Si compared with those with no Si addition. Furthermore, the increase of systolic BP by the CT diet was significantly suppressed by 17 mm Hg (P < .001) in SHR fed the diet containing Mg along with Si, but not by the Mg-deficient diet with or without Si. Soluble silica and CS treatments suppressed the aortic gene expressions of angiotensinogen and growth factors related to vascular remodeling, whereas, Si stimulated the expression of peroxisome proliferator-activated receptor-γ, the activation of which has anti-inflammatory and antihypertensive effects on vascular cells. These findings suggest that Si reduces hypertension in SHR by stimulating the intracellular Mg uptake and related gene expression in the aorta. © 2011 Elsevier Inc.


Shoichi M.,University of Ryukyus | Yukio M.,Okinawa National College of Technology | Koki T.,Okinawa National College of Technology | Yasushi T.,Okinawa Industrial Technology Center | And 2 more authors.
Science and Technology of Welding and Joining | Year: 2012

In fusion welding, gravity makes a molten metal flow downward and it sometimes causes an irregular shaped weld bead and weld defects such as an undercut. To solve this problem, the authors propose a new electromagnetic controlled molten pool welding process method which controls the molten metal flow by using upward electromagnetic forces, and the applicability of this method to industry is examined. In flat position welding with excessive heat input, the molten metal tends to sag down and an undercut defect is likely to occur. It is found that the upward electromagnetic force given by adjusting the conditions of magnetic field can lift the molten metal up, resulting in the remarkably improved shape of a penetration bead. It is further found that, even in overhead position welding, a well shaped penetration bead without undercuts is obtained by adjusting the welding touch angle as well as magnetic field conditions. © 2013 Institute of Materials, Minerals and Mining.

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