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Taira J.,Okinawa National College of Technology | Ohmine W.,Okinawa National College of Technology | Ogi T.,Okinawa Industrial Technology Center | Nanbu H.,University of Ryukyus | Ueda K.,University of Ryukyus
Bioorganic and Medicinal Chemistry Letters | Year: 2012

A novel catechin, pilosanol N (1), was isolated from Agrimonia pilosa Ledeb and its structure was determined by 1H, 13C NMR and HRESI-MS analyses. Compound 1 inhibited nitric oxide (NO) production in LPS/IFN-c -induced RAW264.7 macrophages, and then the iNOS gene expression and its protein production with LPS/IFN-c treatment cells were also suppressed in the presence of 1. In addition, compound 1 scavenged NO or nitrogen radicals generated from NOR3 (4-ethyl-2-hydroxyamino-5-nitro-3-hexenamide) as an NO donor. These results indicated that pilosanol N can decrease the level of NO through a mechanism that involved both a decrease in the NO production and NO scavenging. © 2011 Elsevier Ltd. All rights reserved..

Ugwu C.U.,University of Tsukuba | Tokiwa Y.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Okinawa Industrial Technology Center | Aoyagi H.,University of Tsukuba
Journal of Polymers and the Environment | Year: 2012

The feasibility of utilizing non edible rice (broken rice) for production of fine materials such as poly(3-hydroxybutyrate) (PHB) was considered as one of the alternative ways of keeping the environment clean for sustainable development. Thus, production of PHB from broken rice by simultaneous saccharification and fermentation (SSF) was investigated. During the SSF process, the rice (15% w/v) material was hydrolyzed to glucose, which was utilized by Cupriavidus necator for growth and production of PHB. The PHB content reached 38% at 58 h fermentation. The PHB had weight average molar mass (Mw) and polydipersity index of 3. 82 × 10 5 (g/mol) and 4. 15, respectively. Differential calorimetric scan of the PHB showed a melting temperature (Tm) of 176 °C. Given that the PHB was a homopolymer (which consisted of (R)-3-hydroxybutyric acid monomers), it was thought that broken rice could be a raw material for production of both PHB and (R)-3-hydroxybutyric acid. This SSF process would not only help in the utilization of broken rice or non edible rice, but would also serve as a model for utilization of other raw materials that contain starch for production of PHB. © 2011 Springer Science+Business Media, LLC.

Calabia B.P.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Japan National Institute of Advanced Industrial Science and Technology | Tokiwa Y.,Okinawa Industrial Technology Center | Aiba S.,Japan National Institute of Advanced Industrial Science and Technology
Biotechnology Letters | Year: 2011

Of six strains of lactic acid-producing alkaliphilic microorganisms, Halolactibacillus halophilus was most efficient. It produced the highest concentration and yield of lactic acid, with minimal amounts of acetic and formic acid when sucrose and glucose were used as substrate. Mannose and xylose were poorly utilized. In batch fermentation at 30°C, pH 9 with 4 and 8% (w/v) sucrose, lactic acid was produced at 37.7 and 65.8 g l-1, with yields of 95 and 83%, respectively. Likewise, when 4 and 8% (w/v) glucose were used, 33.4 and 59.6 g lactic acid l-1 were produced with 85 and 76% yields, respectively. l-(+)-lactic acid had an optical purity of 98.8% (from sucrose) and 98.3% (from glucose). © 2011 Springer Science+Business Media B.V.

Rob T.,University of Ryukyus | Ogi T.,University of Ryukyus | Ogi T.,Okinawa Industrial Technology Center | Maarisit W.,University of Ryukyus | And 2 more authors.
Molecules | Year: 2011

Pentylphenols 1 and 2, cyclopropane fatty acid 3, and cyclopentenones 4 and 5, were isolated from an ascidian, Diplosoma sp. The structures of 1-5 were determined by spectroscopic analysis and/or synthesis. Compound 1 inhibited the division of fertilized sea urchin eggs and compound 4 showed mild cytotoxity against HCT116 cells (human colorectal cancer cell).© 2011 by the authors; licensee MDPI, Basel, Switzerland.

Taira J.,Okinawa National College of Technology | Tsuchida E.,Okinawa National College of Technology | Katoh M.C.,Okinawa National College of Technology | Uehara M.,Okinawa National College of Technology | Ogi T.,Okinawa Industrial Technology Center
Food Chemistry | Year: 2015

This study was designed to assess the antioxidant capacity of betacyanins as indole derived plant pigments, such as betanin, phyllocactin and betanidin. The antioxidant capacity of the betacyanins was evaluated as an index of radical scavenging ability using the peroxyl radical generating system in the presence of AAPH and NO generating system using NOR3 as an NO donor. The peroxyl radical scavenging capacity was dose-dependent in the low concentration range (25-100 nM). The mol-Trolox equivalent activity/mol compound (mol-TEA/mol-compound) as an index of the antioxidant capacity indicated the following order at 10.70 ± 0.01, 3.31 ± 0.14 and 2.83 ± 0.01 mol-TEA/mol-compound for betanidin, betanin and phyllocactin, respectively. In addition, betacyanins reduced the nitrite-level in the low concentration range of 2.5-20 μM. The IC50 values (μM) of nitrogen radical scavenging activity were 24.48, 17.51 and 6.81 for betanin, phyllocactin and betanidin. ESR studies provided evidence that the compounds directly scavenged NO. These results indicated that betacyanins have a strong antioxidant capacity, particularly betanidin with a catechol group had higher activity than those of the glycoside of betacyanins. This study demonstrated that the betacyanins will be useful as natural pigments to provide defence against oxidative stress. © 2014 Elsevier Ltd. All rights reserved.

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