Okayama Ichinomiya High School

Okayama-shi, Japan

Okayama Ichinomiya High School

Okayama-shi, Japan
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Nishihira N.,Okayama Ichinomiya High School | Shindo A.,Okayama Ichinomiya High School | Saigusa M.,Okayama University | Ono F.,Okayama University of Science | And 5 more authors.
Journal of Physics and Chemistry of Solids | Year: 2010

The experiment on the search for life under very high pressure done on small animal, tardigrade, has been extended to the moss Ptychomitrium. Several spore placentas of the moss Ptychomitrium were sealed in a small Teflon capsule together with fluorinate as liquid pressure medium. The capsule was put in the center of a pyrophillite cube. This cube was compressed by six tungstencarbide second-stage anvils with a front edge length of 4.0 mm. These anvils were compressed by a first stage, 250 ton press. It was proven that 8090% of the spores were alive and germinated after being exposed to the maximum pressure of 7.5 GPa for up to 48 h. Furthermore, a relatively high germination rate of about 35% was retained even after exposure to 7.5 GPa for 6 days. The pressure tolerance of moss is found to be much stronger than tardigrades. © 2010 Elsevier Ltd.


Ono F.,Okayama University of Science | Mori Y.,Okayama University of Science | Takarabe K.,Okayama University of Science | Nishihira N.,Okayama Ichinomiya High School | And 5 more authors.
Journal of Physics: Conference Series | Year: 2010

It was shown by the present authors group that tardigrade can survive under high pressure of 7.5 GPa. In the case of land plants, however, no result of such experiment has been reported. We have extended our experiments to moss searching for lives under very high pressure. Spore placentas of moss Venturiella were sealed in a small Teflon capsule together with a liquid pressure medium. The capsule was put in the center of a pyrophillite cube, and the maximum pressure of 7.5 GPa was applied using a two-stage cubic anvil press. The pressure was kept constant at the maximum pressure for12, 24, 72 and 144 hours. After the pressure was released, the spores were seeded on a ager medium, and incubated for one week and more longer at 25°C with white light of 2000 lux. It was proved that 70-90% of the spores were alive and germinated after exposed to the maximum pressure of 7.5 GPa for up to 72 hours. However, after exposed to 7.5 GPa for 6 days, only 4 individuals in a hundred were germinated. The pressure tolerance of moss Venturiella is found to be stronger than a small animal, tardigrade. © 2010 IOP Publishing Ltd.


Nishihira N.,Okayama Ichinomiya High School | Iwasaki T.,Okayama Ichinomiya High School | Shinpou R.,Okayama Ichinomiya High School | Hara A.,Okayama Ichinomiya High School | And 8 more authors.
Journal of Applied Physics | Year: 2012

The high pressure technique developed in physics may give a new possibility if it is applied to a biological study. We have been studying the tolerance of small living samples such as planktons and mosses, and found that all of them were alive after exposed to extremely high hydrostatic pressure of 7.5 GPa. This technique has been extended to a higher plant Trifolium lepens L. (white clover). A few seeds of white clover were exposed to 7.5 GPa for up to 6 days. After the pressure was released, they were seeded on agar, or directly on sowing soil. Seventeen out of the total 22 seeds exposed to the high pressure were found to be alive. Those exposed for up to 1 day and seeded on agar germinated roots. Those exposed for up to 1 h and seeded on soil germinated stems and leaves. The present technique has the possibility of being applied to improve breed of plants and to discover a very strong species that stands against very severe environmental conditions. © 2012 American Institute of Physics.


Ono F.,Okayama University of Science | Mori Y.,Okayama University of Science | Sougawa M.,Okayama University of Science | Takarabe K.,Okayama University of Science | And 8 more authors.
Journal of Physics: Conference Series | Year: 2012

We studied the tolerance of living organisms, such as a small animal (Milnesium tardigradum), a small crustacean (Artemia), non-vascular plants or moss (Ptichomitrium and Venturiella), and a vascular plant (Trifolium) to the extremely high hydrostatic pressure of 7.5 GPa. It turned out that most of the high pressure exposed seeds of white clover were alive. Those exposed to 7.5 GPa for up to 1 day and seeded on agar germinated roots. Those exposed for up to 1 hour and seeded on soil germinated stems and leaves. Considering the fact that proteins begins to unfold around 0.3 GPa, it seems difficult to understand that all the living samples which have been investigated can survive after exposure to 7.5 GPa.

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