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Beloglazova N.V.,Ghent University | Shmelin P.S.,OJSC | Eremin S.A.,RAS A.N. Bach Institute of Biochemistry
Talanta | Year: 2016

Three kinds of immunoassays for the determination of gentamicin in milk samples were developed and validated. First, a fast and easily-performed fluorescence polarization immunoassay was used for characterization of the employed polyclonal antibody. The calculated Kaff were (1.9±0.4)×109 M-1 and (6.0±0.2)×106 M-1 for the high- and low-affinity fractions respectively. The assay was characterized with a good sensitivity, the limit of detection being 5 μg kg-1. Two different kinds of detection labels, i.e. colloidal gold (CG) and quantum dots (QDs), were evaluated for use in lateral-flow format with respect to rapid visual on-site testing. The cut-off levels for both qualitative formats were selected based on the maximum level for gentamicin in milk established by the European Commission, 100 μg kg-1, resulting in a 10 μg kg-1 cut-off considering sample dilution. The intra-laboratory validation was performed with sterilized milk samples artificially spiked with gentamicin at concentrations less than, equal to, and greater than the cut-off level. It was shown that milk products could be analyzed without any sample preparation, except for dilution with the buffer solution. The rates of false-positive and false-negative results were below 5% for both labels. The different developed immunoassays were tested towards gentamicin determination in artificially-spiked and naturally contaminated milk samples. © 2015 Published by Elsevier B.V.


Inozemtsev A.A.,OJSC | Dubrovskaya A.S.,Aviadvigatel OJSC | Dongauser K.A.,Aviadvigatel OJSC | Trufanov N.A.,PNRPU
IOP Conference Series: Materials Science and Engineering | Year: 2015

In this paper the process of multiple airfoilvanes manufacturing through investment casting is considered. The mathematical model of the full contact problem is built to determine stress strain state in a cast during the process of solidification. Studies are carried out in viscoelastoplastic statement. Numerical simulation of the explored process is implemented with ProCASTsoftware package. The results of simulation are compared with the real production process. By means of computer analysis the optimization of technical process parameters is done in order to eliminate the defect of cast walls thickness variation. © Published under licence by IOP Publishing Ltd.


Mishin A.A.,Kharkiv Polytechnic Institute | Galchenko V.V.,OJSC
International Conference on Nuclear Engineering, Proceedings, ICONE | Year: 2013

The accuracy and quality of neutron-physical calculations of the active core characteristics depend heavily on the fewgroup constant preparation procedure. The method, based on using average in the fuel assembly fuel and coolant parameters is currently used for preparing macroscopic cross-sections. The question is what impact would considering the uneven distribution of those parameters, made on the few-group constant preparation stage exert on further analysis of the reactor facility behavior during steady-state and transients operation. The study carries out comparative analysis of the neutron-physical characteristics of the VVER-1000 core using the standard approach and using distributed in the fuel assembly fuel and coolant parameters while preparing few-group constants. It's revealed that the fuel pellet and coolant radial temperature distributions affect the multiplication factor and temperature reactivity effect values. Copyright © 2013 by ASME.


News Article | March 4, 2016
Site: www.ogj.com

OJSC Rosneft said it achieved a 124% reserves replacement ratio in 2015, and has a reserve life of 19 years.


News Article | August 22, 2016
Site: www.ogj.com

OJSC Rosneft said its natural gas production of 33.23 billion cu m in the first half of 2016 was 6.9% higher than a year ago, but hydrocarbon liquids declined 0.1%.

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