Oilseeds Research Institute

Faisalābād, Pakistan

Oilseeds Research Institute

Faisalābād, Pakistan
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Alam M.W.,University of Sargodha | Rehman A.,University of Agriculture at Faisalabad | Iqbal Z.,University of Sargodha | Muhammad S.,Government College University at Faisalabad | And 5 more authors.
Plant Disease | Year: 2017

Tomato (Lycopersicon esculentum Mill.) is an important vegetable grown in different parts of Pakistan. Tomato wilts caused by Fusarium oxysporum, Acremonium strictum, and Pseudomonas solanacearum have become threatening diseases of tomato in major growing areas of Punjab Province. In January 2015, diseased plants exhibiting wilt symptoms (drooped leaves and stunted growth) were observed on tomato plants (cv. Money maker) grown in the ground in multiple high tunnels (30 × 180 × 12 ft, W × L × H) located in the Okara (30°48′30.6000″ N; 73°27′33.8256″ E) and Faisalabad (31°25′15.7620″ N; 73°5′21.4584″ E) districts of Punjab Province, Pakistan. Feeder roots of diseased plants exhibited dark-brown to black vascular discoloration. Disease incidence was approximately 20 to 33% out of 60 randomly sampled plants. Symptomatic feeder roots were cut into 3- to 4-mm pieces, surface disinfected with 0.5% sodium hypochlorite for 2 min, rinsed with sterilized water, plated on potato dextrose agar (PDA), and incubated at 25°C with a 12-h photoperiod. Hyphal growth was recovered and pure cultures were obtained by subculturing hyphal tips on fresh PDA plates. Fungal colonies were white to cream-colored initially but then gradually turned pink. Colony surface was slimy and aerial mycelium was sparse. The conidiogenous cells were hyaline, smooth, solitary, phialidic, and the apex of phialides was straight. Conidia were smooth, hyaline, septate or aseptate, elliptical and ovoid, and born on phialides, measuring 6.2 to 9.6 × 1.1 to 2.8 (avg. 8.0 × 2.0) μm. Conidiophores were simple or branched. The isolated fungus was morphologically similar to Plectosphaerella cucumerina (Lindf.) (Carlucci et al. 2012). For molecular characterization, genomic DNA was extracted from a representative isolate (PD10031) and then the internal transcribed spacer (ITS) region was amplified and sequenced using primer pairs ITS1/ITS4. The ITS sequence was deposited to GenBank (Accession No. KY468524). A BLASTn search of the sequence showed 99 to 100% similarity with multiple strains of P. cucumerina isolated from different parts of the world, including an isolate from a greenhouse-grown tomato in China (Xu et al. 2014). Pathogenicity of isolate PD10031 was tested after root-dip inoculation of 27 four-week-old tomato seedlings (cv. Money maker) for 4 min in a spore suspension (1 × 105 spores/ml) prepared from a 7-days-old PDA culture. Inoculated seedlings were then planted into 30-cm-diameter pots containing sterilized potting mix (equal parts sand, peat, and soil), and kept in a greenhouse at 25°C under a 12-h photoperiod. Another set of nine seedlings were root-dipped in sterile water and served as a control. After 12 days, all inoculated plants showed wilt symptoms closely resembling those observed in the tunnel-grown plants. No symptoms were observed on the control plants. The morphology of the reisolated fungus was consistent with the inoculated one. Plectosphaerella cucumerina was firstly reported on tomato in Italy (Matta et al. 1978). To our knowledge, this is the first report of P. cucumerina causing tomato wilt in Pakistan. This disease, if not managed properly, may cause serious economic losses in Pakistan. Future efforts of managing tomato wilt diseases in Pakistan shall take into account this newly reported disease. © 2017, American Phytopathological Society. All rights reserved.


Sajid M.,Bahauddin Zakariya University | Rashid A.,University of Agriculture at Faisalabad | Bashir M.R.,Oilseeds Research Institute | Zeeshan M.A.,University of Sargodha | And 4 more authors.
Pakistan Journal of Phytopathology | Year: 2017

Bacterial leaf Blight of cotton a major seed borne foliar disease that attacks the crop at all growth stages leading to cause severe losses in conducive environment. To find out best source of resistance against pathogen this research was conducted in the field area Department of Plant Pathology, University of Agriculture Faisalabad. Incidence of bacterial blight of cotton was determined on twenty eight varieties/advanced lines sown in field to determine the resistance resource against the disease. Two years data showed that seventeen varieties (BT-Z-33, BT-S-78, BT-786, BT-A-ONE, BT -282, BT-886, BT-3701, BT-SPECIAL, BT-802, Non Bt-FH 901, BT-92, BT-131, BT-905, BT-SUPPER, Non Bt-MNH 496, Non Bt-FH 1000, and BT-121) expressed moderately resistant response with 21-50 % disease incidence (rating 4). Five varieties viz. Non Bt-FH 207, Non BT-N 112, Non BT-FH 942, Non BT-MNH 6070 and Non Bt-FH 941exhibited moderately susceptible response with 51-70% disease incidence under rating 5. Four varieties namely Non BT-N 814, Non Bt-FH 900, Non BT-ANMOL and Non Bt-FH 2015 showed susceptible response with 71-80% disease incidence (rating 6) while Non BT-REDACOLA and Non BT-C 26 expressed highly susceptible response with disease incidence 81-100% rating 7 against bacterial blight disease of cotton. Results indicate that a wide range of resistant resource is present in Pakistan against the disease. The selection of diverse germplasm/resistant varieties through screening is the most efficient, environment friendly and economical management strategy for farmers against this disease. © 2017 Pak. J. Phytopathol.


Iqbal Z.,Oilseeds Research Institute | Pasha I.,National University of Sciences and Technology | Hanif M.S.,Citrus research Institute | Randhawa M.A.,National University of Sciences and Technology | And 2 more authors.
Pakistan Journal of Agricultural Sciences | Year: 2015

Roller flour milling separates wheat components through series of break and reduction rolls along with different sifting operations. Protein (13.70%) and moisture (13.60%) contents were highest in Inqulab 91 break stream blend. Break flour blend of wheat variety Inqulab 91 had Zeleny value of 41mL and gluten content 33% that was found an equivalent to mean for gluten content and Zeleny value in AS 02 break stream blend. Cookie thickness (7.06mm) was highest in cookies made from AS 02 straight grade flour, diameter (25.77mm) and spread factor (41.48) in AS 02 sizing blend while cookie weight (8.24g) in AS 02 break blend. Among sensory characteristics, mean for cookie colour (7.80) was highest in AS 02 break blend while score for crispness (7.80), taste (7.20), surface characteristics (7.60) and overall acceptability (8.00) was maximum in AS 02 middling blend. It is concluded from results that physical parameters of cookie like diameter, spread factor and weight improved with blending while cookie thickness decreased. Cookies made from blended flours exhibited better color, crispness, taste, surface characteristics and overall acceptability. Cookies made from AS 02 middling stream blend were preferred by judges on overall sensory profile basis. © 2015, University of Agriculture. All rights reserved.


Ali M.A.,Ayub Agricultural Research Institute | Zulfiqar A.,Ayub Agricultural Research Institute | Arif A.M.,Ayub Agricultural Research Institute | Khan A.-R.,Ayub Agricultural Research Institute | And 2 more authors.
Agricultural Engineering International: CIGR Journal | Year: 2015

Natural products are more preferred by consumers now-a-days over the synthetic ones. Therefore a natural fruit coating was developed from natural ingredients (shellac, rosin, gum arabic, water and ethanol from sugar industry) without ammonia or morphine as an alternative to the synthetic coatings. So this research was devised to compare the effects of this newly developed natural fruit coating (with 9% total solids) with those of synthetic one (polyethylene based ammonia containing wax with 21% total solids) on the postharvest quality of kinnow (Citrus reticulata Blanco) mandarins. The kinnow mandarins were either coated with natural or synthetic fruit coatings or were left uncoated and stored at 52°C with 85-90% relative humidity for 63 days with five replications for each treatment. The results showed that both fruit coatings significantly (p<0.05) delayed changes in physiological loss in weight, firmness, ascorbic acid and overall sensory quality during the storage period as compared to uncoated fruits, while non-significant (p>0.05) changes in total soluble solids and fruit acidity were recorded for all the treatments. Furthermore the difference between natural and synthetic fruit coatings was non-significant (p>0.05). Therefore, it could be suggested that the natural fruit coating is a good alternative of the synthetic fruit coating. © 2015, Int. Comm. of Agricultural and Biosystems Engineering. All rights reserved.


Hussain M.,Oilseeds Research Institute | Tahir M.S.,Oilseeds Research Institute | Sharif M.,Oilseeds Research Institute | Tahira,Oilseeds Research Institute
Pakistan Journal of Agricultural Sciences | Year: 2011

The promising strain RBN-03052 is an outcome of hybridization (KS-75 x Rainbow) and selected through pedigree method of selection since 1998-99. Homozygous progenies of pedigree number F6-6052 were bulked in 2003-04 for yield evaluation. Its performance was evaluated through station, zonal, micro yield trials and National Uniform Rapeseed Yield Trials. RBN-03052 has proved its worth in all yield trials and its performance is better than all the existing cultivated varieties. Its maximum potential of 2833 kg/ha was achieved in 2005-06 at Oilseeds Research Station, Khanpur. It produced 33.96% higher yield in micro yield trials carried during 2006-07. Similarly, its yield surpassed the checks with 2.80% and 10.27% in National Uniform Rapeseed Yield Trials carried out during 2006-07 to 2007-08 respectively. The new strain showed tolerance against insect pests and diseases specially Alternaria blight, Powdery mildew, Downy mildew and White rust. Agronomic studies revealed that this variety fits in a better way with the existing agronomic package of technology. The new strain RBN-03052 named as Punjab Sarson is first canola version variety developed locally and is released for commercial cultivation in both irrigated and barani regions of the Punjab. © 2012 Pakistan Journal of Agricultural Sciences, Faisalabad, Pakistan. All rights reserved.

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