Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital

Baoding, China

Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital

Baoding, China
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Sun J.-J.,Tangshan Union Hospital | Zhang P.,Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital | Zhou G.-Q.,Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013

BACKGROUND: Edaravone can scavenge free radicals and inhibit lipid peroxidation, which can improve microenvironment injury after central nervous system injury. OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cell transplantation combined with edaravone on the treatment of cerebral infarction in rats. METHODS: The models of cerebral infarction were made by middle cerebral artery occlusion. Model rats were randomly divided into control group, stem cell group (bone marrow mesenchymal stem cell transplantation) and combination group (edaravone+bone marrow mesenchymal stem cell transplantation). The concentration of 2.0×106/mL bone marrow mesenchymal stem cell suspension or the concentration of 1 mL cell culture fluid was injected into the rat models through the caudal vein; at the same time, 3 mg/(kg•d) edaravone aqueous solution or normal saline was injected into the rat models through the abdominal cavity, for 5 days. The neurobehavioral scores were determined after 24 hours, 3 days and 1, 2 weeks after modeling. Aquaporin 9 and aquaporin-4 mRNA expressions were determined by reverse transcription-PCR. The rats were sacrificed for neuron and brain tissue around target morphological analysis by hematoxylin-eosin staining under a light microscopy. RESULTS AND CONCLUSION: There was no difference in the neurological deficit scores among the three groups at 24 hour and 3 days after treatment (P > 0.05); while after 2 weeks, the neurological deficit score in the combination group was significantly lower than the stem cell group and control group (P < 0.05-0.01). In the stem cell group, the Aquaporin 9 and aquaporin-4 mRNA expressions were higher than the combination group but lower than the control group (P < 0.05). The number of CM-Dil-positive cells and neurons was higher in the combination group than the stem cell and control groups (P < 0.05). These findings indicate that transplanted bone marrow mesenchymal stem cells can survive and migrate to the peri-infarct region, and differentiate into neuron-like cells. Bone marrow mesencyhmal stem cell transplantation combined with edaravone can significantly improve neurological function of cerebral infarction rats.


Zhang P.,Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital | Zhou G.-Q.,Oil Geophysical Prospecting Bureau of Trade Unions Central Hospital | Sun J.-J.,Tangshan Union Hospital
Chinese Journal of Tissue Engineering Research | Year: 2013

Background: Simple bone marrow mesenchymal stem cell transplantation in repair of damaged brain tissues does not exhibit ideal effect. Objective: To investigate the effect of Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of cerebral infarction in rats. Methods: The models of middle cerebral artery occlusion were established by suture method and randomly divided into three groups. Model group received tail vein injection of PBS. Danhong injection group received tail vein injection of 2 mL/kg Danhong injection. Combination group received injection of 2 mL/kg Danhong injection + 2.0×109/L bone marrow mesenchymal stem cell suspension, for 5 consecutive days, once a day. Results and Conclusion: At 2 weeks following bone marrow mesenchymal stem cell transplantation, the neurological function scores in the combination group were significantly better than those in the model group and Danhong injection group (P < 0.05). Cerebral infarct volume in the combination group was significantly less than that in the model and Danhong injection groups at 3 weeks following transplantation (P < 0.05). Histopathological observation showed that the reduced degree of tissue injury was larger in the combination group than the Danhong injection and model groups. Results indicated that Danhong injection combined with bone marrow mesenchymal stem cell transplantation on the treatment of rats with cerebral infarction showed remarkable effects and protected brain cells.

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