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Di Gennaro A.,OIE Reference Laboratory for West Nile Disease | Lorusso A.,OIE Reference Laboratory for West Nile Disease | Casaccia C.,OIE Reference Laboratory for West Nile Disease | Conte A.,OIE Reference Laboratory for West Nile Disease | And 2 more authors.
Clinical and Vaccine Immunology | Year: 2014

A serum neutralization assay (SN) was compared with the official plaque reduction neutralization test for the quantitation of West Nile virus antibodies. A total of 1,348 samples from equid sera and 38 from human sera were tested by these two methods. Statistically significant differences were not observed, thus supporting the use of SN for routine purposes. Copyright © 2014, American Society for Microbiology. All Rights Reserved. Source


Savini G.,OIE Reference Laboratory for West Nile Disease | Puggioni G.,Istituto Zooprofilattico Sperimentale della Sardegna | Di Gennaro A.,OIE Reference Laboratory for West Nile Disease | Di Francesco G.,OIE Reference Laboratory for West Nile Disease | And 8 more authors.
Epidemiology and Infection | Year: 2013

SUMMARY West Nile virus (WNV) strains belonging to lineage 2 were detected and isolated from the tissues of a goshawk and two carrion crows in Sardinia in August 2012. According to NS3 sequence analysis, the Sardinian isolates shared a high level of similarity with those of Italian lineage 2 strains which circulated in 2011 and with the homologous sequence of the 2004 Hungarian isolate. Following the human fatality reported in 2011 in Olbia, this study is the first to report the spread and enzootic circulation of WNV lineage 2 in Sardinia. Copyright © Cambridge University Press 2013. Source

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